Molecular Devices QPix 450, User Manual

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Chapter 14: Replication Processes
The QPix 450 Colony Picking System or QPix 460 Colony Picking System is capable of
replicating colonies between plates.
The following options are available in the Navigation window under Replication Processes:
Library Replication replicates between plates that have the same number of wells.
Library Compression replicates from 96-well plates to 384-well plates, compressing the
samples.
Library Expansion replicates from 384-well plates to 96-well plates, expanding the samples.
Note: Before you run a replicating process, it is important that you do the cleaning and
set up procedures in
Preparing to Run Processes on page 29 .
If this is the first replication process, you must edit or create a Sanitise profile to use with the
replication process. See
Creating and Editing Sanitise Profiles on page 51 .
Creating and Editing Replication Processes
The procedures for creating and editing the different types of replicating processes are similar.
Differences and exceptions are identified within the instructions when applicable.
If this is your first replication process, you must edit or create a Sanitise profile to use with the
replication process. See
Creating and Editing Sanitise Profiles on page 51 .
Creating and editing a replication process involves the following procedures:
Opening the Replication Window on page 190
Selecting Replication Routines on page 190
Selecting Barcode Options on page 192
Selecting the Plate and Sanitizing Options on page 194
on page 194
Viewing the Settings Summary on page 195
Changing the Picking Head on page 196
After you create the routine, you can close the process and run it later, or you can run it
immediately.
Note: Some steps that are included in these procedures might not be available,
depending on the features included with the instrument and license.
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