Molecular Devices FLIPR Tetra, User Manual

Page: 27 / 302

FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 27
Consumables and Accessories on page 283 for recommended tips.
Cell Suspension
The Cell Suspension option consists of two components:
• The Cell Reservoir installable in Position 4 (Source 3) in the 5
position stage.
• The Cell Suspension module located externally on the right side
of the instrument.
The Cell Reservoir (see Figure 2-5 ) is user installable. The Cell
Suspension module (see Figure 2-6 ) consists of a shelf with a magnetic
motor mounted underneath it, a cell flask with a magnetic stirrer, up to
four fluid bottles for automated cleaning, and a removable cover for
keeping cells in a dark environment.
Figure 2-7 demonstrates how the system is connected and shows all
the possible combinations for protocol development.
Figure 2-5 Cell Reservoir.
The Cell Reservoir, which the user places in a source plate location, is
filled from any of the bottles in the external Cell Suspension module by
a pump with adjustable speed and direction.
The Cell Reservoir is a special plate type that has one fluid line used for
both input and output, and an electronic plate ID that is part of the
electrical/fluidic connector which identifies the reservoir to the system.
In the case of an overflow, an overflow trough catches excess fluid and
pipes it to the tip washer overflow trough, which directs it to the
overflow bottle. The reservoir is also autoclavable for cleaning
purposes.
The spinner flask contains a stirrer, which is driven by a magnetically
coupled motor mounted in the lower part of the Cell Suspension
module. The Cell Suspension stir speed can be set in a protocol or with
manual instrument controls. Stir speed of 5 equals approximately 1