Molecular Devices FLIPR Tetra, User Manual

Page: 192 / 302

Running an Experiment
192 0112-0109 H
Experimental Setup Parameter
Fluid Transfer Settings—Aspirate
Parameter Setting
Read Mode Luminescence
Excitation Wavelength None
Emission Wavelength None
Camera Gain
1
1. May be adjusted to compensate for cell brightness or saturation problems.
70,000–280,000
Exposure Time
1
0.53 s
LED Excitation Intensity NA
Gate Open 100%
Read Interval 1 s
Reads before pipetting 5
Reads during first interval 50–90
Save Assay Images Possible, but creates very large files
Parameter
Suspension Setting
(pipette cells)
Adherent Settings
(pipette compound)
Cell Flask Spinning Rate Manual setting, Speed 6 NA
Fluid Transfer Type
Single Aspirate, Single
Dispense
Single Aspirate, Single
Dispense
Source Plate
Fill Reservoir speed
Drain Reservoir speed
Drain destination
defaultcellres384
5
4
Cell flask
Source Plate 2
NA
NA
NA
Aspiration Volume 25 μ L 25 μ L
Aspiration Height 70 μ L 5 μ L
Aspiration Speed 20 μ L/s 20 μ L/s
Tip Up Speed 10 mm/s 10 mm/s
Hold Volume
1
0 μ L 0 μ L