Molecular Devices FLIPR Tetra, User Manual

Page: 144 / 302

Exchanging Hardware
144 0112-0109 H
Proceed to the following procedure to reset the system.
1. Select the Change Optics command from
Instrument/Manual Operation menu and click OK . This will
reset the instrument.
2. Following the reset, an optics calibration will be necessary. See
Recalibrating the Optics on page 150 .
Installation of Plate Hold-Down Devices
The plate hold-down devices, part numbers 2300-1631 and 2300-1633,
are designed to keep plates or tip racks from lifting after aspirating,
dispensing and tip loading. Each device has an overhang feature that
contacts the skirt of a plate to prevent upward movement when the
pipettor is removed from the plate. Three different plate hold-down
gaps (2 mm, 2.5 mm and 6 mm) are provided that correspond to three
identified plate skirt thicknesses. The 2.0 mm and 2.5 mm are
incorporated on one plate hold down device that may be rotated 90×. A
second plate hold-down has a 6.0 mm gap and can be found in the
accessory kit. Numbers corresponding to the plate skirt thicknesses are
marked on the top of the devices. The device is properly oriented for a
particular flange thickness when the digits can be read as upright from
the service door of the instrument.
Selection of Appropriate Plate Hold-Down
Most plates and tip racks use the 2.5 mm device setting. Exceptions
among approved plates are the Corning/Costar plates that have a very
thick 6.0 mm plate skirt and the thin 2.0 mm plate skirt of thee Greiner
1536-well plates. To determine which hold-down device is most
appropriate for your plate, use the lowest hold-down device that allows
the plate skirt to slide under the overhanging feature freely. It is
important the overhanging feature is clearly above the skirts on all
plates and tip racks that will be used in a particular plate position or a
misalignment with the pipette tips will occur. Test the configuration as
described below after installation.
Note: If a desired filter is not available, three custom filter
cassettes are available for purchase (see Optics Consumables
on page 284 ) to place filters created by an outside vendor. Once
installed, these filters appear as 1_1, 2_2, 3_3 in the software
protocol.