Molecular Devices FLIPR Tetra, User Manual

Page: 127 / 302

FLIPR
®
Tetra High Throughput Cellular Screening System User Guide
0112-0109 H 127
Wash Cell Reservoir Process
This process is only available when the Cell Reservoir is installed and is
recommended for use before and after using cells in suspension.
This process can be used as a way of priming the reservoir with cells
prior to an assay or for cleaning purposes.
No pipetting is associated with this step.
The table below describes the options available in the
Wash Cell
Reservoir process page.
Settings/Options Description
Pre-coat Tubes Checking this box will pump the fluid up to the sensor
before filling the reservoir.
Fluid Source Select which fluid to use for the wash process. All
Fluid 1–4 bottles are available as well as the Cell
Flask.
Drain Reservoir
Destination
Select a drain destination for the fluid left in the
reservoir after the wash. Waste bottles, Cell Flask, or
any of the Fluid bottles are available.
Note: This option is only available when the
Cell Reservoir is installed and selected as the
source plate for aspiration.
Fill Reservoir Speed Select a pump rate for filling the Cell Reservoir; the
range is 1–10.
Note: This option is only available when the
Cell Reservoir is installed and selected as the
source plate for aspiration.
Drain Reservoir Speed Select a pump rate for draining the Cell Reservoir; the
range is 1–10.
Note: This option is only available when the
Cell Reservoir is installed and selected as the
source plate for aspiration.
Wash Cycles Set how many wash cycles with the above settings
should be done.
Hold Time This option will soak the reservoir in the pumped fluid
by leaving the fluid in the reservoir for a specified
time; the range is 0–300 seconds (0–5 minutes).