EMax® Plus Microplate Reader Quick Start Guide
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7
Setting Up an Absorbance Endpoint Experiment
You can define the setting for your absorbance endpoint experiment in the SoftMax Pro
Software.
1. Turn on the instrument and start the SoftMax Pro Software. See
Instrument and the Software on page 3
.
2. Select an instrument. See
Selecting the Instrument in the Software on page 4
3. With the
Plate
section active, click
Settings
on the
Home
tab in the ribbon.
4. Click
ABS
read mode.
5. Click
Endpoint
read type.
6. Select the wavelengths for the read.
Select up to six wavelengths.
Select the wavelengths from the list of installed filters.
7. Select the wells in the microplate to read.
You can choose to read an entire microplate or a subset of wells.
To select a contiguous, rectangular region on the microplate, drag the cursor to
select the wells to be read.
Columns do not need to start with “1” but must be contiguous.
To read all the wells in the microplate, select the
Select All
check box.
Note:
If you select a subset of wells, the instrument reads the entire
microplate, but reports the data for the selected wells only.
8. Click
OK
.
9. Save the settings as a data file or a protocol file. See the SoftMax Pro Software
application help or user guide.
Data files contain the experiment settings, the raw data collected by your
reader, and the data analysis you might have completed.
Protocol files are experiment template files that contain microplate well
layout assignments and all other reader configuration information, but no
data.
After creating and saving the settings for your experiment, you can insert the prepared
microplate in the microplate carrier and start the read. See
