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minipcr blueGel User Manual Download Page 2

02.

[email protected]
bluegel

TM

 user’s guide

TABLE OF CONTENTS

Overview

 Features
 Components

Directions for use

 

Casting a gel

 

Running a gel

 

Document the run

Troubleshooting and maintenance

 Troubleshooting
 

Care & maintenance

 

Specifications & operating conditions

03

03
04

07

07
09
10

11

11
12
13

INDEX

Summary of Contents for blueGel

Page 1: ...yus LLC miniPCR bio and the miniPCR bio logo are trademarks of Amplyus LLC bluegelTM user s guide support minipcr com blueGel TM Electrophoresis System User s guide Integrated electrophoresis and visualization system ...

Page 2: ...LE OF CONTENTS Overview Features Components Directions for use Casting a gel Running a gel Document the run Troubleshooting and maintenance Troubleshooting Care maintenance Specifications operating conditions 03 03 04 07 07 09 10 11 11 12 13 I N D E X ...

Page 3: ...ation Amber filter integrated in cover for direct visualization View results within minute Casting System Save on reagents 20 ml gels and 30 ml buffer 60 x 60 mm gel tray with one or two rows of combs Two double sided combs with choice of 9 and 13 wells Easy to Operate and Store Intuitive two button operation one for Run one for Light 3 x 9 inch footprint 4 inches high Storage pouch included Fold ...

Page 4: ...04 support minipcr com bluegelTM user s guide C O M P O N E N T S COMPONENTS Cover Gel Tray Buffer Chamber Base ...

Page 5: ...05 support minipcr com bluegelTM user s guide Combs Gel Tray Casting platform COMPONENTS Cont C O M P O N E N T S ...

Page 6: ...E N T S COMPONENTS Cont Comb storage under casting platform System includes 1 Cover 2 Gel trays 1 Buffer chamber 1 Base 1 Casting platform 2 Two sided combs 1 Power supply 1 ClearViewTM Spray 1 Lens cleaning cloth 1 Fold a ViewTM Imaging Hood 1 Carrying pouch ...

Page 7: ... for additional DNA staining dyes that work with blueGelTM Weigh the desired amount of agarose according to the chart above and add it to a 100 ml size flask or larger containing 20 ml of 1X TBE electrophoresis buffer Mix well by swirling Tip If pouring more than one gel agarose and buffer quantities can be multiplied by the number of gels to be poured Increase heating time by 15 sec per additiona...

Page 8: ...h the gel in a refrigerator Do not disturb the gel during this time After the gel has solidified remove comb s gently by pulling straight upward Remove the gel tray from the casting platform If a small amount of gel has formed underneath the gel tray wipe it off and discard it TIP We recommend using gels immediately after casting Unused gels can be kept at ambient temperature for 5 days if they ar...

Page 9: ...trophoresis spray one pump or less of ClearView SprayTM inside the orange cover between the electrodes Spread to an even coat using a microfiber cloth Wipe gently do not rub clean Place the orange cover on the blueGelTM base The cover contains the electrodes and will only fit in one direction with the electrode positioned to attract the negatively charged DNA Press the power button to start the ru...

Page 10: ... in bright ambient light use the supplied Fold a ViewTM photo documentation hood Pop up the Fold a ViewTM following the instructions on its side and place it on the blueGelTM orange cover sliding it down until it fits snugly around the cover s edges Place your camera on top and align the camera lens with the circular opening on the Fold a ViewTM If needed softly wipe condensation off the inside of...

Page 11: ...rientation with the tab towards the back of the unit Orange cover won t fit ensure proper orientation Run won t start LED indicator not on check electrode contact and alignment between cover and base Check that the running buffer is making contact with the electrodes Confirm that you are using the correct running buffer Condensation on cover apply ClearViewTM before use or use lens cleaning cloth ...

Page 12: ...elTM system in optimal working condition Rinse the casting platform combs gel tray buffer chamber and cover in distilled water after each use Do not wipe or handle platinum wire Air dry Never submerge the blueGelTM base in water Do not use ethanol or organic solvents to clean parts Handle the buffer chamber gel tray and cover with care to protect from scratching Always store blueGelTM components i...

Page 13: ...with blueGelTM Description Part No GelGreen Agarose Tabs RG 1500 10 GelGreen DNA stain RG 1550 01 Agarose 20g RG 1500 02 TBE Buffer 20X RG 1502 02 blueGel base QP 1500 05 blueGel cover QP 1500 06 blueGel casting platform QP 1500 07 blueGel gel tray QP 1500 08 blueGel comb QP 1500 09 blueGel buffer chamber QP 1500 10 blueGel power supply QP 1500 11 blueGel carrying pouch QP 1500 12 blueGel Lens cle...

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