Setup
Reservoir Assembly/Disassembly
1. Remove pressure relief valve (if installed) and slide reservoir out of retaining stand.
Disassemble and clean all components prior to first time use.
2. Attach a 5 in. (12.7 cm) piece of ¼ in. (6.4 mm) outer diameter (OD) tubing to the liquid
outlet port (labeled "L") on the bottom of the reservoir cap.
Liquid port
tubing
Liquid outlet port (L)
Gas port (G)
Pressure relief valve
Sample port
Cap
Retaining stand
Base
Reservoir O-ring
Reservoir O-ring
Pressure relief
valve O-ring
Reservoir body
3. Ensure that the reservoir O-rings are positioned correctly in the grooves of base and cap. If
needed, lubricate O-rings lightly with water or petroleum jelly. Do not allow petroleum jelly
to come in contact with sample.
NOTE:
Inspect O-rings for cuts or wear; if necessary, replace with spare O-rings.
4. Push base and cap into reservoir body until properly seated, then slide reservoir assembly
into retaining stand.
WARNING:
Never use reservoir without its retaining stand! The cap will pop off when
reservoir is pressurized and liquid in reservoir may splatter.
Inlet/Outlet Tube Fitting Assembly
The polyethylene tubing required for assembling the reservoir with the stirred cell and selector
valve is supplied with the following products. Tubing designations listed below refer to the
ports on the Amicon® Stirred Cell Selector Valve.
RES IN and RES OUT port tubing is supplied with the Amicon® Stirred Cell Reservoir.
CELL tubing is supplied with the Amicon® Stirred Cell (quick-connect fitting on one end).
GAS SUPP tubing is supplied with the Amicon® Stirred Cell Selector Valve.
NOTE:
Attachment to older model stirred cells is the same, except that the cell cap connection
is the tubing assembly shown on the next page, rather than quick-connect.
Operation
WARNING:
Do not exceed pressure limit of 5.2 bar (75 psi). During diafiltration, the pressure
should not exceed 3.8 bar (55 psi), in order to maintain equilibrium between the
stirred cell and the reservoir. If the liquid level increases slightly, concentrate
briefly.
To prevent fluid from entering the stirred cell pressure relief valve during
diafiltration, do not exceed the maximum working volume
(e.g., 50 mL for the 50 mL stirred cell).
NOTE:
Nitrogen gas is recommended for pressurizing the Amicon® Stirred Cell and reservoir.
Refer to Amicon® Stirred Cell Selector Valve user guide for detailed instructions on
concentrating and diafiltering with the selector valve and reservoir.
1. To concentrate, push the
GAS spool valve plug on the selector valve in until spool plug seats
fully.
2. Initiate stirring and slowly pressurize the reservoir. Check connections for leaks, then
increase pressure as desired. Both the reservoir and stirred cell are pressurized, but the cell
is "seeing" only gas and there is no flow of liquid from the reservoir to the cell.
3. To diafilter, first run in
GAS mode (steps 1 and 2) for 5–10 seconds, then push the LIQ spool
valve plug in. This allows pressurized liquid to flow out of the reservoir and into the stirred
cell.
4. Proceed with concentration or diafiltration until desired sample volume or buffer exchange
has been achieved.
Shutdown and Disassembly
1. Turn off pressure at source, then turn off magnetic stirrer.
2. Vent pressure from system using the reservoir pressure relief valve, rather than the stirred
cell pressure relief valve. This prevents liquid remaining in tubing/valve between cell and
reservoir from flowing into the cell.
3. To disassemble, disconnect gas and liquid tube fittings.
4. Remove pressure relief valve and slide reservoir out of retaining stand.
5. Pry top and bottom caps from reservoir sleeve using cap removal tool provided.
6. Remove O-rings from top and bottom caps if needed.
Cleaning and Maintenance
1. After each use, wash the reservoir and all fluid-carrying tubing with mild laboratory
detergent and rinse with deionized water.
2. If tube fitting assemblies are removed for any reason, rewrap the threads with
polytetrafluoroethylene tape before replacing them back into the cover. Use moderate force
when replacing fittings.
Sterilization/Disinfecting
The reservoir is
NOT autoclavable. To sterilize, use standard sterilizing gas mixtures. To
disinfect, use 5% formalin. Do
NOT use alcohol.
Additional tubing may be needed if using the reservoir with other devices.
1. Lay out the stirred cell, selector valve, reservoir, and four pieces of tubing in positions
appropriate for their use together. Estimate length of tubing required for each connection
and cut to length if necessary.
2. Carefully unscrew each of the four hex caps from the selector valve body and remove the
O-ring. A grab ring and spacer are included inside each hex cap.
3. Slide the hex cap and grab ring (flat portion facing
hex cap) onto one end of each of the four pieces of
tubing leaving no more than ½ in. (1.3 cm) of tubing
beyond grab ring. Add a spacer (countersink toward
grab ring) and O-ring onto the end of each piece of
tubing.
4. Attach each tubing assembly to the appropriate
selector valve port and hand tighten the hex caps.
5. Add appropriate tube fittings to the free ends of
RES IN, RES OUT, and GAS SUPP tubing
pieces. The
CELL tubing already has a quick-connect fitting on it.
6. Refer to the diagram below and connect the ends of the tubing as follows:
● Connect
GAS SUPP to external pressure source.
● Connect
CELL to quick-connect inlet fitting on stirred cell.
● Connect
RES IN to inlet fitting (labeled "G" on Amicon® Stirred Cell Reservoir cap).
● Connect
RES OUT to outlet fitting (labeled "L" on Amicon® Stirred Cell Reservoir cap).
7. Assemble the Amicon® Stirred Cell according to the user guide and fill with sample.
8. Add sample or diafiltration buffer to the reservoir through the recessed sample port. Install
pressure relief valve, taking care not to cross-thread the valve in the sample port.
200
175
150
125
100
75
60
50
40
30
20 ml
250
50
100
150
200
GAS
GAS
SUPP
CELL
RES IN
RES OUT
LIQ
100
800
700
600
500
400
300
200
Amicon® Stirred Cell
Reservoir
(cat. no. 6028)
Amicon® Stirred Cell
Selector Valve
(cat. no. 6003)
Amicon® Stirred Cell
To regulated
pressure source
½ in.
Grab ring
O-ring
Spacer
Tubing
Hex cap
Setup,
continued
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