Millipore Sigma Pellicon 3 Cassettes Installation And User Manual Download Page 12

Pellicon

®

 3 Cassettes Installation and User Guide 

12

importance is stable process flux and no carry over.  
If the NWP decreases significantly from run-to-run, cleaning procedures may be 

inadequate.  Alternative cleaning agents and procedures should be investigated. 

Contact a Technical Service Representative for assistance.

NWP Temperature Correction Factor (F)*

T (°F) 

T (°C)

F

T (°F)

T (°C)

F

T (°F)

T (°C)

F

125.6

52

0.595

96.8

36

0.793

68.0

20

1.125

123.8

51

0.605

95.0

35

0.808

66.2

19

1.152

122.0

50

0.615

93.2

34

0.825

64.4

18

1.181

120.2

49

0.625

91.4

33

0.842

62.6

17

1.212

118.4

48

0.636

89.6

32

0.859

60.8

16

1.243

116.6

47

0.647

87.8

31

0.877

59.0

15

1.276

114.8

46

0.658

86.0

30

0.896

57.2

14

1.310

113.0

45

0.670

84.2

29

0.915

55.4

13

1.346

111.2

44

0.682

82.4

28

0.935

53.6

12

1.383

109.4

43

0.694

80.6

27

0.956

51.8

11

1.422

107.6

42

0.707

78.8

26

0.978

50.0

10

1.463

105.8

41

0.720

77.0

25

1.000

48.2

9

1.506

104.0

40

0.734

75.2

24

1.023

46.4

8

1.551

102.2

39

0.748

73.4

23

1.047

44.6

7

1.598

100.4

38

0.762

71.6

22

1.072

42.8

6

1.648

98.6

37

0.777

69.8

21

1.098

41.0

5

1.699

*Based on Water Fluidity Relative to 25 ºC (77 ºF) Fluidity Value  F= (µ

T ºC

25 ºC

) or 

T ºF

77 ºF

)

Storage

Storage solution must be introduced into 

the system through the cassettes and 

the system piping to put the system in a 

bacteriostatic state in between process 

runs. This procedure requires a minimum 

of 10 L/m

2

 of solution. Refer to 

Typical 

System Setup

 

diagram.

1.  Close the tank drain valves (V1 and V2). 
2.  Fill the tank with the storage 

solution.  

3.  Fully open the retentate valve 

(V4) (and permeate valve (V5) 

if present) and direct both the 

retentate and filtrate to the tank. 

4.  Open the tank drain valve (V1).
5.  Turn on the feed pump and pump 

water into the feed port of the 

Pellicon

® 

 holder. The recommended 

feed flow rate for Pellicon

® 

 3 

cassettes is 4 to 6 L/min/m².

6.  Once the pump has slowly ramped 

up to the set point, partially close 

the retentate valve (V4) to achieve 

the retentate pressure listed 

here

.

7.  Recirculate the storage solution for 

5 to 10 minutes.

Summary of Contents for Pellicon 3 Cassettes

Page 1: ...ale Holder Installation 4 Hydraulic Process Scale Holder Installation 5 Maintenance 7 Flushing and Cleaning 7 Flushing 8 Cleaning 8 Sanitization 9 Depyrogenation 10 Integrity Testing 10 Measurement of...

Page 2: ...TE Pellicon 3 88 cm2 and 0 11 m2 Cassettes must be installed in a Pellicon Cassette Holder Catalog Number XX42PMINI Holder Installation 1 Loosen the nuts on the tie rods of the holder and remove the n...

Page 3: ...wipe 4 Slide the manifold adapter plate onto the holder against the holder s manifold plate with the solid light grey side labeled Manifold Side towards the manifold and the wings resting on the tie...

Page 4: ...ary Tri Clover connection Pellicon Cassette hex nut washer stainless steel bushing handle PERMEATE RETENTATE FEED Pellicon10_2_06_2 eps Gasket Plate manifold adapter plate nut PERMEATE R E T E N T A T...

Page 5: ...wrench and 15 16 in deep hex socket turn each nut 1 8 turn in the sequence shown in Figure 4 When the wrench clicks this indicates that the set force has been reached DO NOT OVER TORQUE Evenness of to...

Page 6: ...d tight 4 Turn on the air supply to the Hydraulic Power Unit A minimum of 80 psig air pressure should be available Check the air pressure on the pressure gauge PI 1 on Hydraulic Power Unit front panel...

Page 7: ...ut replacement of nuts and washers should be considered after every ten installations to maintain high sealing force in the holder 7 When new nuts do not spin freely refurbish the threaded rods with a...

Page 8: ...se the tank drain valve V1 and the tank valve V2 2 Fill the tank with the flushing solution 3 Fully open the retentate valve V4 and permeate valve V5 if present and direct both the retentate and filtr...

Page 9: ...iofilms Biopolymer Proteins and Polyphenolic Membrane Cleaning Agent Concentration Temperature C pH Time min Ultracel NaOH 0 1 N 0 5 N 25 50 13 13 7 30 60 Biomax NaOH 0 1 N 0 5 N 25 50 13 13 7 30 60 N...

Page 10: ...c Acid 0 1M 30 50 1 30 NaOH 0 1N 25 50 13 30 Ultracel NaOH 0 1N 25 50 13 30 Use milder conditions if effectiveness of agent is still observed Better membrane life is expected with lower concentration...

Page 11: ...l the tank with filtered deionized water water for injection or reverse osmosis permeate The flush water must be extremely pure to avoid fouling the membranes or introducing other contaminants into th...

Page 12: ...0 40 0 734 75 2 24 1 023 46 4 8 1 551 102 2 39 0 748 73 4 23 1 047 44 6 7 1 598 100 4 38 0 762 71 6 22 1 072 42 8 6 1 648 98 6 37 0 777 69 8 21 1 098 41 0 5 1 699 Based on Water Fluidity Relative to...

Page 13: ...ess compression and ensures that the units will not leak externally NOTE The storage agent used must comply with applicable local regulations Recommended Storage Solutions for Pellicon 3 Cassette Filt...

Page 14: ...completely flushed Flush system and cassette System and cassette not completely cleaned Clean system and cassette Symptom Possible Cause Remedy High NWP Damage to membrane or cassette Integrity test...

Page 15: ...xed below fluid air interface Vortex in feed container Add baffle to break vortex or reduce mixing speed Retentate splashing Fix retentate line below fluid in feed air interface container Pump cavitat...

Page 16: ...atory matters to the best of our knowledge and ability but without obligation or liability Existing laws and regulations are to be observed in all cases by our customers This also applies in respect t...

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