Setup
General Guidelines
● Do not use the SNAP i.d.® 2.0 IHC system for
deparaffinization, rehydration, dehydration, or mounting
steps.
● Proper slide preparation is critical to successful use.
Before
placing tissue on slide, make sure that it will not
extend beyond the target area of the assembly fixture.
●
IMPORTANT!
Do not touch the gasket on the slide holder
during assembly.
Slide Holder Setup
1. Place assembly fixture on a hard, flat surface.
2. Position slide in assembly fixture, with the tissue
section facing up. Verify that the tissue section does
not extend beyond the target area.
3. Hold the slide holder by the sides, clip side down, and
align it with the assembly fixture containing the slide.
Do not touch the gasket
.
4. Press the slide holder down firmly over the slide until
the four compression clips slip over the slide edges
with an audible click.
5. To ensure that all clips are fully engaged, firmly press
down on the top edge of each slide holder corner, one
at a time, and listen for a click on each press.
Click 4
Click 1
Click 2
Click 3
6. Remove the slide holder assembly from the fixture
and closely inspect each clip to confirm that it is
in the proper position over the slide. If any clip is
not engaging the slide as shown below, place the
assembly back into the fixture and repeat step 5 on the
unengaged clip(s).
Clip engaged
Clip not engaged
IHC Frame Setup
The IHC frame can be set up either on or off the
SNAP i.d.® 2.0 system.
1. Place assembled slide holders into any vacuum ports
on the frame, with slide labels oriented towards the
port numbers.
2. Press blue port plugs
lightly
into all unused ports
(excessive force is not required). Unused plugs can be
left in the port plug storage holes.
3. If the frame has been loaded off the SNAP i.d.
®
2.0
system, place it in either position on the system before
continuing.
Staining Protocol
Tips for adding solutions to slide holder
● Small volumes (up to 600 µL) should be dispensed with
a 1 mL pipette through the injection/recovery port.
Gently
push the pipette tip into the port to create a seal.
Dispense slowly to avoid bubble formation in the tissue
section chamber.
● Larger volumes (up to 6 mL) can be loaded into the wash
reservoir.
● Make sure that the fluid completely covers the tissue
sections.
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