Luminex Luminex 200, System User Manual

Page: 51 / 64

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MAP Technology
Troubleshooting
41
Acquisition
Problems
Symptom Possible problem Solution
Acquisition fails or slows. The air pressure is out of range. See Pressurization on page 35.
The sample probe height is
incorrect. Adjust the sample probe height.
See page 18.
The sample probe is clogged. Clean the sample probe. See
page 20.
The sheath bottle has a leaky
seal. Make sure that the sheath bottle
lid is tightened. Remove and
replace the sheath bottle lid.
The sheath or waste lines are
not fully connected. Disconnect and reconnect the
lines; you should hear them
click.
The calibration microspheres
have expired. Replace old microspheres with a
fresh lot.
The wrong wells are selected for
the calibration microspheres. Ensure that the correct wells are
selected in the Maintenance tab.
The calibration lot number or
target values selected in setup is
incorrect. Enter the correct calibration lot
number and target values in the
Update CAL Targets box.
Slow or unsuccessful sample
acquisition. The sample probe is clogged. Clean the sample probe. See
page 20.
The air pressure is out of range. See Pressurization on page 35.
The sample probe is not
vertically aligned. Adjust the sample probe height.
See page 18.
Air is present in the system. Verify sample probe height. Run
three Prime commands, two
Alcohol Flush commands, then
three Washes with water.
The acquisition volume is set
too high. Set the acquisition volume to at
least 25 µL less than the actual
volume in your wells. This
setting lets the analyzer acquire
sample more efficiently with
less chance of acquiring air.