logos biosystems Luna-II yf User Manual Download Page 29

 

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Chapter 8 - Purchaser Notification   

8.1 Limited Use Label License: Research Use Only 

The purchaser of this product should use this product only for research for the sole benefit of the purchaser. By use of 
this product, the purchaser agrees to be bounded by the terms of this limited use statement whether the purchaser is a 
for-profit or a not-for-profit entity. 

If the purchaser is not willing to accept the conditions of this limited use statement and this product is unused, the 
Company will accept return of the product with a full refund. 

The purchaser cannot resell or otherwise transfer (a) this product (b) its components or (c) materials made using this 
product or its components to a third party for Commercial Purposes. 

Commercial Purposes mean any and all uses of this product and its components by a party for monetary or other 
consideration, including but not limited to, (a) product manufacture, (b) providing a service, information, or data, (c) 
therapeutic, diagnostic, or prophylactic purposes, or (d) resale of this product or its components whether or not such 
product and its components are resold for use in research. 

Logos Biosystems, Inc. (“Company”) will not claim any consideration against the purchaser of infringement of patents 
owned or controlled by the Company which cover the product based on the manufacture, use or sale of a therapeutic, 
clinical diagnostic, vaccine, or prophylactic product developed in research by the purchaser in which this product or its 
components was employed, provided that neither this product nor any of its components was used in the manufacture of 
such product.   

For any use other than this limited use label license of research use only, please contact the Company or email 
[email protected] for more information. 

 

8.2 Instrument Warranty 

 

Logos Biosystems, Inc. (“Company”) warrants to the original purchaser (“Purchaser”) that the instrument (“Instrument”), 
if properly used and installed, will be free from defects in materials and workmanship and will conform to the product 

specifications for a period of one (1) year (“Warranty Period”) from the date of purchase. If the Instrument under this 
limited warranty fails during the Warranty Period, the Company, at its sole responsibility, will: 

1)  within and up to 30 calendar days of purchase, refund the purchase price of the Instrument to the Purchaser if 

the Instrument is in original conditions; or, 

2)  after 30 calendar days of purchase, only replace or repair the Instrument for up to the Warranty Period without 

issuing a credit. 

In no event shall the Company accept any returned instrument (including its components) that might have been used or 
contaminated in some labs, including but not limited to, HIV or other infectious disease or blood-handling labs. This 
limited warranty does not cover refund, replacement, and repair incurred by accident, abuse, misuse, neglect, 
unauthorized repair, or modification of the Instrument. This limited warranty will be invalid if the Instrument is 
disassembled or repaired by the Purchaser. 

In case that the Company decides to repair the Instrument, not to replace, this limited warranty includes replacement 
parts and labor for the Instrument. This limited warranty does not include shipment of the Instrument to and from service 
location or travel cost of service engineer, the costs of which shall be borne by the Purchaser. Every effort has been 
made to ensure that all the information contained in this document is correct at its publication. However, the Company 
makes no warranty of any kind regarding the contents of any publications or documentation as unintended or 
unexpected errors including occasional typographies or other kinds are inevitable. In addition, the Company reserves the 
right to make any changes necessary without notice as part of ongoing product development. If you discover an error in 
any of our publications, please report it to your local supplier or the Company. The Company shall have no responsibility 
or liability for any special, incidental, indirect or consequential loss or damage resulting from the use or malfunction of 
the Instrument.   

This limited warranty is sole and exclusive. The Company makes no other representations or warranties of any kind, 
either express or implied, including for merchantability or fitness for a particular purpose with regards to this Instrument. 

To obtain service during the Warranty Period, contact your local supplier or the Company’s Technical Support team. 

OUT OF WARRANTY SERVICE 

Please contact your local supplier or the Company’s technical support team in order to obtain out-of-warranty service. If 
necessary, repair service will be charged for replacement parts and labor hours incurred to repair the Instrument. In 
addition, the Purchaser is responsible for the cost of shipping the Instrument to and from the service facility and, if 
necessary, the travel cost of a service engineer. 

Summary of Contents for Luna-II yf

Page 1: ...User Manual www logosbio com LBSM MD ML LUY 001 VL1712 01...

Page 2: ...d can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a res...

Page 3: ...r 3 Protocol Settings 13 3 1 Protocol Parameters 13 3 2 Creating and Editing Protocols 14 3 3 Protocol Selection 15 Chapter 4 Counting Yeast Cells 16 4 1 Instrument Preparation 16 4 2 Sample Preparati...

Page 4: ...erly connected If the instrument is not grounded the electrical safety of the product cannot be guaranteed 8 Turn the instrument on only after connecting the power cord and AC adapter to both the powe...

Page 5: ...inutes of mixing samples for accurate cell viability measurements If necessary count your sample twice duplicate readings and take an average 3 As the LUNA II YF is calibrated before shipping recalibr...

Page 6: ...F also provides the option to review previous data that can be exported to USB as a CSV file Both reusable and disposable slides can be used with the LUNA II YF Designed for cost efficient an accurate...

Page 7: ...sing option at the specified concentration of Saccharomyces cerevisiae Component Quantity LUNA II YF Automated Yeast Cell Counter 1 Power Cord with AC Adapter 1 PhotonSlide 50 Slides 1 box Acridine Or...

Page 8: ...hscreen a power button a slide port to insert slides and a USB port for easy data transfer 1 5 2 Rear View of the LUNA II YF The rear of the LUNA II YF has two USB ports and a power inlet to connect t...

Page 9: ...Do not install the instrument in a location that will expose the instrument to intense ultraviolet light 2 2 Startup Main Menu Push the power button below the touchscreen to turn the instrument on The...

Page 10: ...the most recent calibration and the date and version of the latest software update Settings options 2 3 1 Save Options Preset the types of files saved and customize the file name prefix 2 3 2 Dilutio...

Page 11: ...f live and dead cells Raw Image Untagged images of cells in each channel Report PDF report with count data and histograms Select the desired saving options by pressing the boxes in front of each optio...

Page 12: ...o use for subsequent dilution calculations Press Dilution Calculator Options in the Settings screen Select the desired value The selection will be marked with a yellow circle Press to return to the Se...

Page 13: ...t remove the slide or turn off the instrument during this process The background calibration value and date will have changed in the Settings Screen 2 3 5 Settings Software Update Logos Biosystems con...

Page 14: ...ctively see Section 4 2 Sample Preparation Assuming a final sample volume of 20 L users can modify this value according to the following table Yeast Volume 20 L 18 L 17 L 16 L 15 L 14 L 12 L 10 L 8 L...

Page 15: ...be modified or deleted To create a new protocol select one of the default protocols and press Save as Using the onscreen keyboard rename the protocol and press Save The newly created protocol will ap...

Page 16: ...tate and Propidium Iodide select the FDA PI protocol Select the desired protocol in the Protocol screen Press Load to apply the selected protocol Now the instrument is ready to count cells with the se...

Page 17: ...in Pipette gently Incubate the sample for 10 minutes at room temperature FDA PI Mix 17 L yeast suspension with 1 L Fluorescence Signal Enhancer 1 1 L Fluorescein Diacetate Stain and 1 L Propidium Iodi...

Page 18: ...l appear to the right of the screen The GF button indicates that the green fluorescence channel is on Press GF to change it to RF and switch to the red fluorescence channel To view the stained cells u...

Page 19: ...the image The red outer box in the top left corner of the image represents the entire counting area and the inner box is the current field of view The size and location of the inner box will change w...

Page 20: ...channel and a merged image of both channels Press Overlay to change it to GF and display live cells Press GF to change it to RF and display dead cells Press RF to change it to Overlay and display a me...

Page 21: ...y the distribution of only live cells Press Live on to change it to Dead on and display the distribution of only dead cells The LUNA II YF provides a gating function that can be controlled by the gati...

Page 22: ...nt concentration options are Total Live Dead and Custom Users may set the concentration to be used for the dilution calculation by pressing the grey box below the Current Concentration value to be the...

Page 23: ...tings see Section 2 3 1 Settings Save Options Using the onscreen keyboard name the count as desired A default file name prefix may be set in the Settings 2 3 1 Settings Save Options Users may add the...

Page 24: ...s Avg size indicates the fluorescent object size Cell Count Report File name AOPI_07032016_161208 Date 07 MAR 2016 16 08 Cell count results Total 7 09 x 10e5 cells mL Live 6 60 x 10e5 cells mL Dead 4...

Page 25: ...es the fluorescent object size If available in the folder thumbnails of captured images will appear below the results Tap on the thumbnails to make it full size Users can view the raw images captured...

Page 26: ...sh the power button for five seconds 6 2 Cleaning Turn the LUNA II YF off and disconnect the power cable before cleaning Make sure that liquids do not enter any part of the instrument during cleaning...

Page 27: ...otocol or create a new protocol Make sure the appropriate dilution factor has been selected Poorly aligned images Wait for cells to settle in the slide chambers prior to counting Data transfer and sav...

Page 28: ...x 0 5 mL F23002 Acridine Orange Stain 2 x 0 5 mL F23003 Propidium Iodide Stain 2 x 0 5 mL F23004 Propidium Iodide Stain for Yeast 2 x 0 5 mL F23211 Fluorescein Diacetate Stain 2 x 0 5 mL F23213 Fluore...

Page 29: ...strument is in original conditions or 2 after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period without issuing a credit In no event shall the Company ac...

Page 30: ...31 478 4184 Email sales logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 517 266 3925 Email sales logosbio com EUROPE 11B avenue...

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