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Last Updated on Sep 2021 

 

Under any circumstances, users are not allowed to make bookings on behalf of other 
people. Users who have received training but are yet to gain access to microscopy 
resource booking may approach TLL Bioimaging department for booking assistance if they 
need to use it urgently.  

 

If a user fails to show up within the first 30 minutes of their booking, the slot is forfeited 
and is free for any user to use it.  

 

If any users violate any of these Booking rules, users will be subjected to the 3 strikes 
policy. 

 

3.1 Acknowledgements 

If you use the TLL Microscopy and Imaging facility and/or have been trained or assisted any of 
the bioimaging facility members in your research, then this should be acknowledged 
appropriately in your publications and presentations. 

4. Operation Procedures 

Every confocal system in TLL Bioimaging Facility has its specific instructional manual which are 
found in every confocal rooms. Strictly adhere to the correct order of operation for all system. 
Failure to do so will result in disciplinary action from the facility. Any issues encountered during 
the operation of the system are advised to seek help from the Bioimaging facility.  

Modification, exchange or removal of components beyond this operational manual is strictly 
prohibited and is only carried out by the manufacturer, Bioimaging facility or by experienced 
users approved by bioimaging facility. During operation of laser microscopy system, do not look 
into the laser beam directly as they are all Class 3b and Class 4 lasers.   

 

 

 

1st offence 

A warning will be issued along with the reminder of the rules. 

2nd offence 

A second warning will be issued and your respective PI will be notified. 

3rd offence 

Banned from using any of the facility’s microscope for 2 weeks.

 

Summary of Contents for TCS SP8

Page 1: ...ng and System Access 5 3 1 Acknowledgements 6 4 Operation Procedures 6 4 1 Switching ON Protocol 7 4 2 Objective Lens 7 4 3 Software initiation 8 4 4 Turning on the lasers 8 4 5 Using Dye Assistant ta...

Page 2: ...Last Updated on Sep 2021 THIS PAGE IS INTENTIONALLY LEFT BLANK...

Page 3: ...ensure the lasers have sufficient time to heat up and cool down before running again When using the lasers to image NEVER look directly at the laser while imaging If a safety shutter is present make s...

Page 4: ...hen you notice that a bulb has reached between 2 000 2 500 hours or is not functioning properly 2 Training Use of confocal microscopy and the online booking system is authorized by facility staff only...

Page 5: ...week but it has to be shared across the systems that they have received training on Each booking must not exceed 3 hours If users have utilized all their entitled bookings for the week 24Hr Rule can b...

Page 6: ...Operation Procedures Every confocal system in TLL Bioimaging Facility has its specific instructional manual which are found in every confocal rooms Strictly adhere to the correct order of operation fo...

Page 7: ...re ON for a minimum of 2hrs before switching OFF as it requires sufficient time for warming up STED laser You may switch ON up to 100 Make sure the STED lasers are ON for at least 1hr before switching...

Page 8: ...4 4 Turning on the lasers 1 Under the Visible panel click on the button to open the laser panel 2 Click on the UV 405nm Visible Argon and SuperContVisible2 WLL bar to observe 405nm Argon and WLL respe...

Page 9: ...ector HyD or PMT you would like to image with your dyes HyD is more sensitive than PMT 4 The Dye assistant will give you a list of laser settings options to select from Select the settings which has t...

Page 10: ...our fluorophore Important Make sure that the emission band is at least 8 10nm away from your excitation line Choose an appropriate detector pseudocolour also known as a Look Up Table or LUT For PMT se...

Page 11: ...tional X Useful for live imaging unless you require fast imaging in which case you will need to adjust the phase correction Zoom is where you control the zoom parameters the arrows allow you to nudge...

Page 12: ...e background black level Pinhole the wider the pinhole aperture the thicker is the optical slice which means a blurrier but brighter image Make sure you also lower the laser power if you have to incre...

Page 13: ...3D Image Scroll down to the Z stack option in the Acquisition tab Select Live to see the specimen Use the focus knob on the USB control panel or point the cursor in the Z stack box panel and use the m...

Page 14: ...e any images which are older than 3 months Please make sure to do a backup Option 2 In TLL research cmn drive folder in your respective lab folders Option 3 In your own lab drive Option 4 In your pers...

Page 15: ...ethanol to remove any oil residues e In the event of logging off you may leave the lasers ON and exit LASX Otherwise Switch off all lasers within the software Diode Laser OFF 405nm White Light laser...

Page 16: ...s issue is a scanner issue you will not hear clicking as the program is shutting down and performing its pre shutdown WLL checks 2 Switch off the LSM switch 3 marked Scanner 3 Switch on the LSM 4 Open...

Page 17: ...If it says the HyD overloaded use the following steps 1 Turn off Live View 2 Ensure that the detection band is set at least 10nm away from any laser you are using 3 Set the HyD gain to 100 always Brig...

Page 18: ...Last Updated on Sep 2021 5 1 Troubleshooting Appendix Switches under computer terminal Power schematic diagram of the SP8...

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