121
Selecting the Laser
Figure 102:
MP Laser Configuration, second shutter open
3. You can now set the wavelength of the MP laser. Use either the slider (
)
or enter the desired wavelength directly.
4. Select a suitable neutral filter strength for the light incidence (
Trans
) and/or define the
Gain
and
Offset
from the EOM (
).
5. The position of the multifunction port (
MFP
) should be set to
SP680
or
SP700
.
6. For beam splitter systems, set the
beam splitter
to
substrate (Figure 103
).
Figure 103:
Setting the beam splitter to substrate
15.1.3
Using Pulsed VIS Lasers
1. Switch on the laser driver (PDL 800, SEPIA) as instructed in the corresponding manual.
2. Select
External
as the trigger on the front of the laser driver or, when using a SEPIA II,
in the corresponding driver software.
3. Set the intensity to a value between 3.5 and 9 (on the lever on the front side of the PDL
driver) or between 35 % and 90 % (in the SEPIA II software driver).
Summary of Contents for TCS SP8 SMD
Page 1: ...10 Living up to Life User Manual Leica TCS SP8 SMD for FCS FLIM and FLCS ...
Page 4: ...4 Copyright ...
Page 14: ...14 Contents ...
Page 18: ...18 Intended Use ...
Page 20: ...20 Liability and Warranty ...
Page 28: ...28 General Safety Notes ...
Page 32: ...32 Additional Notes on Handling the System ...
Page 44: ...44 System Overview and Properties ...
Page 60: ...60 SMD Components Figure 31 DSN 102 Dual SPAD Power Supply ...
Page 80: ...80 Safety Features ...
Page 102: ...102 Switching On the System ...
Page 116: ...116 LAS AF ...
Page 214: ...214 Changing the Specimen ...
Page 216: ...216 Changing the Objective ...
Page 238: ...238 Switching Off the System ...
Page 242: ...242 Repairs and Service Work ...
Page 244: ...244 Maintenance ...
Page 246: ...246 Disassembly and Transport ...
Page 248: ...248 Disposal ...
Page 254: ...254 Contact ...
Page 256: ...256 Recommended Literature ...
Page 266: ...266 Appendix Figure 225 Declaration of conformity ...
Page 268: ...268 Appendix ...
Page 269: ......