CIAN Leica SP8 – short instructions, version 3.1, September 2015
page 3 of 10
2.2 - Cleaning objectives
Clean immersion objectives before and after use. The dry objectives on the SP8 should not be
cleaned in this fashion since they have concave front lenses.
Use
dry lens paper
to blot off larger amounts of oil or other immersion medium:
•
Take a sheet of lens paper, fold it in three into a long rectangle; do not run your fingers
over the middle of the paper that will touch the lens
•
Wipe the lens by holding either end of the lens paper, dragging it gently across the
objective lens three times. Use a fresh area of the tissue each time.
•
Repeat as needed with a fresh lens paper to remove excess of immersion oil.
Use
wet lens paper
to remove traces of oil, other dirt:
•
Fold as above, put a drop or two of lens cleaner (the blue fluid) on the lens paper.
•
Wipe the lens gently by holding either end of the wet lens tissue dragging it gently across
the objective lens three times. Use a fresh area of the tissue each time.
•
Repeat with water.
NEVER wipe the lens in a circular pattern; NEVER apply any pressure directly to the lens.
Note:
If you find something on the lens that you can’t remove with this procedure,
please
contact CIAN staff as soon as possible, and discontinue use of the objective
until the
problem is solved
3 - Operating Leica SP8
3.1 - Manual controls
Note that you can use the TL and WFF functions independent of the computer and the
scanner/laser power.
1: WFF filter cubes (blue, green, red)
2: WFF shutter
3: status display
4: XYZ joystick (
SmartMove
) with separate
coarse/fine toggle buttons for x/y, z
5: TL activation, toggle BF, DIC, Pol
6: focus knob
7: Field aperture (AP) control
8: BF light intensity
9: move objectives up/down; set Z
zero/limits (hold ‘set’, push up arrow twice)
10: XYZ coarse/fine toggle
Fig 4
: Microscope front and side panels
