8) Move the turret as down as it goes by z down button on
the right side of the microscope and check the stage’s Z
displacement. Objective glasses and plates have focus
roughly 3,5mm from bottom and Mattek dishes have
focus about 1mm above that.
9) Once the software has started, chose Configuration/Lasers and turn on all
the lasers you will use. If you are using Argon laser, adjust its power to 20%
from the slider. If you want to save your data in other than 8 bit format
(recommended if you plan to quantify your data), go to
Configuration/Settings and choose the 12 bit option.