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Leica S Series 

User Manual 

42

Checking parfocality

1.  Select the highest magnification level.

2.  Monitor the specimen; if necessary, refocus 

it slightly.

3.  Change from the highest to the lowest 

magnification. The sharpness should be 
constant (parfocal). If this is not the case, 
repeat this procedure.

Dioptric correction with two adjustable eyepieces (continued)

Summary of Contents for S Series

Page 1: ...Living up to Life Leica S series User Manual ...

Page 2: ... use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abra ded particles O O In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Servicing O O Repairs may only be carried out by Leica Microsystems trained servi...

Page 3: ...afety maintenance and accessories The Safety Concept booklet contains additi onal safety information regarding the service work requirements and the handling of the stereomicroscope accessories and electrical accessories as well as general safety instruc tions You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and...

Page 4: ... mandatory to read and observe Failure to comply can cause the following O O Hazards to personnel O O Functional disturbances or damaged inst ruments Danger due to hot surface This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes O O This symbol wi...

Page 5: ...utside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use O O Refer to Safety Concept booklet O O Electrical components must be placed at least 10 cm away from the wall and from flammable substances O O Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort m...

Page 6: ... for shipping or transporting the individual modules of the Leica S stereomicroscopes and the acces sory components O O In order to prevent damage from vibra tions all moving parts that according to the user manual can be assembled and disassembled by the customer should be disassembled and packed separately Integration in third party products O O Refer to Safety Concept booklet Disposal O O Refer...

Page 7: ...e workload must be taken O O Optimal arrangement of workplace work assignments and work flow changing tasks frequently O O Thorough training of the personnel giving consideration to ergonomic and organizati onal aspects O O The ergonomic optical design and const ruction of the Leica S stereomicroscopes are intended to reduce the exertion of the user to a minimum Direct contact with eyepieces is a ...

Page 8: ...rt Guide A stereomicroscope of the S series in overview 27 Tips for working ergonomically 28 Using the Eyepieces 29 The correct interpupillary distance 30 Focusing 31 Switching magnification zooming 32 Limiting zoom range 33 Regulating the resistance of the focusing drive 35 Changing the position of the optics carrier 36 Diopter settings and parfocality 1 adjustable 1 fixed eyepiece 37 Dioptric co...

Page 9: ...ries User Manual 9 Specifications Overview of technical features 52 Specifications 53 Appendix Calculating the total magnification field of view diameter 55 Troubleshooting 56 Care Maintenance Contact Persons 57 ...

Page 10: ...Leica S Series User Manual 10 Leica S series ...

Page 11: ...aneously large working distan ces and object fields you can always view your microscopic specimens accurately from the complete image to the finest detail Though the reliability and robustness of Leica stereomicroscopes is world renowned like any high tech product the Leica S series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the...

Page 12: ...e The Leica S series provides a high degree of flexibility in choosing equipment thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades The optics carriers eyepieces stands and more can be combined in any way you choose allowing you to create the stereomicroscope that best suits your needs Despite this you will notice that the contro...

Page 13: ...uding its cold light source and stand is made from highly conductive material with surface resistivity of 2 10 11 Ohm square with a discharge time of 2 seconds 1000 V to 100 V The Leica S6 T terminator version for highly complicated working ranges and the T incident light stand consist of a dissipative material with a surface resistivity of 102 106 Ohm square and a discharge time of 0 1 seconds fr...

Page 14: ...Leica S Series User Manual 14 The model series Leica S4 E Leica S6 E Leica S6 Leica S6 T Leica S6 D Leica S8 APO ...

Page 15: ...n assembled and commissioned by your Leica consultant click here to skip through the installation instructions and go directly to the Quick Start Guide on Page 26 If on the other hand you are assembling the stereomicroscope yourself continue with the Assembly chapter which begins on Page 16 ...

Page 16: ...Leica S Series User Manual 16 Assembly ...

Page 17: ...ment overview 1 Sub base for transmitted light with glass stage plate 2 Incident light base with stage plate 3 Focusing column with microscope carrier 4 Additional objective optional 5 StereoZoom optics carrier 6 Eyepieces fixed and or adjustable 6 5 4 3 2 1 ...

Page 18: ...ght side of the focusing column Focusing column Focusing column on the incident light base 1 Remove the stage plate 2 Route the 3 hollow screws from below through the base plate and screw these securely into the focusing column 3 Insert the stage plate back into place ...

Page 19: ...ith the connection screw 4 Press the locking mechanism backward The incident light base and the sub base for transmitted light are now connected 5 Insert the glass stage plate 6 Insert the universal light guide in the opening at the rear Additional information can be found in the User Manual for cold light source Leica KL300 LED Sub base for transmitted light and cold light source ...

Page 20: ...t in the desi red position with the clamping screw Additional objective optional 1 Screw the desired objective counterclock wise into the optics carrier Protective objective glass optional 1 Screwtheobjectiveprotectiveglassdirectly onto the StereoZoom or on the additional objective Optics carrier and additional objective ...

Page 21: ...the eyepiece in place Available Graticules The following graticules and objective micro meters for calibrating may be ordered O O Graticule 10 mm 0 1 mm O O Graticule 5 mm 0 1 mm O O Graticule 5 mm 0 05 mm O O Graticule 100 Div 0 002 O O Graticule 100 Div 0 001 O O Graticule 150 Div 0 0005 O O Crosshairs O O Stage micrometer 50 mm 0 1 0 01 mm graduation O O Stage micrometer 1 0 001 graduation Avai...

Page 22: ...the side on which the scale is inscribed The scale must not appear reversed 2 Remove the insert from the bottom of the eyepiece and place it on the bench with the knurled side down 3 Hold the graticule by the edges to avoid leaving fingerprints and push it into the holder from the side 4 Replace the insert in the eyepiece and press it firmly into place 5 Insert the eyepiece in the tube and turn th...

Page 23: ...p the high powered StereoZoom Leica S8 APO with two adjustable eyepieces Inserting the eyepieces 1 Push eyepieces as far as they will go into the tubes 2 Check that the eyepieces are seated firmly and precisely in place Risk of infection Direct contact with eyepieces is a poten tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using persona...

Page 24: ...r detailed information about installation and use please refer to the user manual for the Leica KL300 LED Please note that the universal light guide on the Leica S8 APO can only be used with the light arm mounted on the side High output illuminators For higher demands such as photogra phyorinusecombinationwiththeLeica S8 APO we offer different high performance transmitted light stands and LED inci...

Page 25: ...accessories and the software packages from Leica can be found in the corresponding manuals Installing the camera 1 Remove the protective dust cover from the video projection lens C mount adapter and the microscope camera 2 Screw the camera into the video projection lens C mount 3 Insert the unit into the video photo output of the stereomicroscope and screw it in Always close the video photo output...

Page 26: ...Leica S Series User Manual 26 Quick Start Guide ...

Page 27: ... carrier in the microscope carrier 5 Adjustable tube Interpupillary distance adjustable from 55 to 75 mm 6 Eyepieces 7 Threads for fastening the light arm both sides and rear 8 Illumination Leica LED3000 SLI 9 Illumination Leica LED3000 RL 10 Leica microscope camera 11 C mount adapter and video photo tube 1 7 8 9 10 5 6 4 3 11 2 An overview of an S series stereomicroscope ...

Page 28: ...carrying out other tasks perform exercises to relax and relieve muscle tension Ergonomic objectives The ErgoObjective for the Leica S4 E and all S6 models provides for fatigue free work The ErgoObjective 0 6 0 75 with an adjustable working distance of 77 137 mm and the Ergo Objective 0 7 1 0 with an adjustable working distance of 48 98 mm allow the fine adjust ment of the working distance the magn...

Page 29: ...ersonal dioptric correction for the user Dioptric Correction In order to parfocally match the stereomic roscope at least one eyepiece with dioptric correction is necessary The setup is described on the following pages O O With one adjustable and one fixed eyepi ece from page 37 O O With two adjustable eyepieces from page 40 If you do not wear glasses Depending on the preferences of the observer ey...

Page 30: ... it will become automatic Reference value The interpupillary distance can be set between 55 and 75 mm An exit pupil distance is the distance between eye and eyepiece With the 10 23B wide field eyepiece for eyeglass wearers it is approx 22 mm For those who do not use the eyepiece for eyeglass wearers it is 12 mm Setting the eye distance 1 Bring the eyes slowly to the eyepieces 2 Push the tubes toge...

Page 31: ...ctive The focusing drive can be operated either left or right handed Focusing 1 Align the specimen under the objective 2 Set the lowest magnification level At the lowest magnification the desired specimen detail can be easier to localize due to the large field of view 3 Look into the eyepieces and insert the desi red specimen detail in the center 4 Focus on the specimen with the drive knob Focusin...

Page 32: ... can be operated with the left and the right hand The image scale is shown on the right drive knob The basis for the calculation of the total magnification and the field of view can be found on page 26 Changing magnification 1 Look into the eyepieces 2 Focus on the object see page 16 3 Rotate the magnification changer until the desired magnification is configured ...

Page 33: ...on level can be set The following example shows the limit to the range between 1 to 3 2 Defining the lower limit 1 Loosen the hollow screw on the left drive knob with the Allen key provided 2 Turn the right drive knob to position 1 3 Set the stop on the left drive knob forward until it touches the built in zoom stop 4 Carefully tighten the Allen screw Continued on next page ...

Page 34: ...drive knob until it touches the built in zoom stop 4 Carefully tighten the allen screw Defining the upper limit stop 1 Loosen the hollow screw on the right drive knob with the Allen key provided 2 Turn the right drive knob to position 3 2 Limiting zoom range continued ...

Page 35: ...tend to slide downwards The resistance can be adjusted individually depen ding on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focu sing Regulating the resistance of the focusing drive ...

Page 36: ...ide ways in the microscope carrier if the user wants to work from the side Changing position 1 Unscrew the clamping screw 2 Turn the optics carrier laterally to the desi red position 3 Carefully tighten the clamping screw Changing the position of the optics carrier ...

Page 37: ...this advantage as often as possible it is not available on all stereomicroscopes The diopters can be set between 5 and 5 The following adjustments have to be carried out only once by each user Using reticules leads to slightly deviated settings which are described in the user manuals on the reticules measurement Adjusting the diopter settings 1 With Leica S6 D and Leica S8 APO turn the rotary knob...

Page 38: ...he other eye into the into the adjustable eyepiece 11 Rotate the eyelens of the eyepiece slowly in the direction until the eye can see the specimen sharply Continued on next page 6 Set the highest magnification level 7 Optimize the focusing with the focusing drive 8 Set the lowest magnification level 3 Place a flat specimen under the objective 4 Set the lowest magnification level 5 Observe the spe...

Page 39: ...xed eyepiece continued Checking parfocality 1 Select the highest magnification level 2 Monitor the specimen if necessary refocus it slightly 3 Change from the highest to the lowest magnification The sharpness should be constant parfocal If this is not the case repeat this procedure ...

Page 40: ...o view a specimen detail that is located higher or lower Use this advantage as often as possible it is not available on all stereomicroscopes The diopters can be set between 5 and 5 The following adjustments have to be carried out only once by each user Using reticules leads to slightly deviated settings which are described in the user manuals on the reticules measurement Adjusting the diopter set...

Page 41: ...in the direction until this eye sees the specimen sharply 12 Repeat steps 10 and 11 with the other eye Continued on next page 6 Set the highest magnification level 7 Optimize the focusing with the focusing drive 8 Set the lowest magnification level 3 Place a flat specimen under the objective 4 Set the lowest magnification level 5 Observe the specimen through the eyepi eces and bring it into sharp ...

Page 42: ...gnification level 2 Monitor the specimen if necessary refocus it slightly 3 Change from the highest to the lowest magnification The sharpness should be constant parfocal If this is not the case repeat this procedure Dioptric correction with two adjustable eyepieces continued ...

Page 43: ...Leica S Series User Manual 43 Photography Video ...

Page 44: ...FC cameras If you require absolute control over the camera and need the capability for measurement evaluation and more in addition to photogra phy the digital Leica DFC cameras are exactly right for you Together with the Leica Applica tion Suite they provide virtually limitless free dom of use For additional information about Leica cameras refer to the camera s documen tation Leica Application Sui...

Page 45: ...00 of the light crosses the video photo beam path The focusing and the framing are done with the left eyepiece video photo beam path Flat specimens are sometimes partially blurry on the left and right edge of the image This blurring is based on laws of optics and does not mean there is a malfunction of the camera or the microscope Capturing images and videos 1 If the image detail and image sharpne...

Page 46: ...Leica S Series User Manual 46 Dimensional drawings in mm ...

Page 47: ...eica S6 E S4 E S6 T with incident light and transmitted light illumination 161 107 25 81 110 351 3 8 22 223 278 278 ø58 ø76 46 144 104 113 148 205 205 223 161 107 25 81 110 351 3 8 22 65 278 ø58 ø76 46 144 104 113 148 205 220 278 205 ...

Page 48: ...Leica S Series User Manual 48 Leica S6 120 148 25 81 110 390 6 0 ø15 8 25 26 35 ø58 ø76 46 144 104 150 4 ...

Page 49: ...l 49 Leica S6 D with incident light and transmitted light illumination 207 107 25 81 110 351 3 8 22 223 278 278 ø58 ø76 144 104 113 148 205 205 171 207 107 25 81 110 351 3 8 22 65 278 ø58 ø76 144 104 113 148 205 220 278 205 171 ...

Page 50: ... 50 Leica S8 APO with incident light and transmitted light illumination 207 107 25 116 75 351 3 8 22 278 ø58 ø76 144 104 113 148 205 278 205 171 207 107 25 116 75 351 3 8 22 65 223 278 ø58 ø76 144 104 113 148 205 220 278 205 171 ...

Page 51: ...Leica S Series User Manual 51 Technical data ...

Page 52: ... 3 40 60 ErgoObjective Leica S6 T 6 3 1 6 3 40 38 Terminator ErgoObjective Leica S6 D 6 3 1 6 3 40 38 Video photo tube ErgoObjective Leica S8 APO 8 1 10 80 38 Apochromatic Greenough system Apochromatic zoom Apochromatic objectives Video photo tube StereoZoom is a trademark registered in the Principal Register of the US Patent and Trademark Office Overview of technical features ...

Page 53: ...432 lp mm 600 lp mm Maximum num About the Aperture 0 124 0 144 0 144 0 144 0 144 0 2 Working distance basic config 110 mm 110 mm 110 mm 110 mm 110 mm 75 mm Object field diameter 36 5 mm 36 5 mm 36 5 mm 36 5 mm 36 5 mm 23 mm Adjustable zoom limits 2 2 2 2 2 Video photo outlet switchable 100 visual or 100 video photo and 100 visual in the left eyepiece 100 visual or 100 video photo and 100 visual in...

Page 54: ...Leica S Series User Manual 54 Appendix ...

Page 55: ...ification changer position NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10 23 16 16 20 12 10 23B 16 14B 25 9 5B 40 6B Example MO Additional objective 1 6 ME 20 12 eyepiece z Zoom position 4 0 Magnification in the binocular tube MTOT VIS MO ME z or 1 6 20 4 128 Calculation example Field of view diameter in the specimen OF NFOV 12 1 9 mm MO z 1 6 4 ...

Page 56: ...s always first check O O Is the voltage selector set correctly O O Is the main power switch switched on O O Is the power cable connected correctly O O Are all connecting cables attached correctly O O Are the fuses intact Photos are blurry O O Focus accurately P 23 O O Bring the reticule into sharp focus and perform diopter correction exactly accor ding to the instructions P 37 O O Insert the eyepi...

Page 57: ... careless or improper handling Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Schweiz AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com Care O O Protect your stereomicroscope from mois ture fumes and acids and from alkaline caustic and corrosive materials and keep chemical...

Page 58: ...s Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsu itable cleaning agents and techniques can damage polymers Permitted measures O O Clean the stereomicroscope or parts of it using warm soapy water then wipe using distilled water O O For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing s...

Page 59: ...n forensic science investigations and educational applications Leica Microsystems an international company with an experienced worldwide customer service network Internationally active Phone Fax Australia North Ryde 61 2 8870 3500 2 9878 1055 Belgium Diegem 32 2 790 98 50 2 790 98 68 Denmark Ballerup 45 4454 0101 4454 0111 Germany Wetzlar 49 64 41 29 40 00 64 41 29 41 55 England Milton Keynes 44 8...

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