Leica DM E, Instruction Manual

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5
3.0 Proper Adjustment
1 Reset the eyepieces, then plug in the microscope power cord and turn it on.
2 Lower the stage using the microscope focus adjustment control knob and put a standard
stained microscope slide on the stage.
3 Rotate the nosepiece to move the 10x objective into working position.
4 Raise the stage using the microscope coarse adjustment focus knob until you reach its
positive stop. Using the fine adjustment knob, bring the specimen into sharp focus.
5 Adjust the eyetubes for interpupillary distance and eye difference.
6 Adjust for eye difference focus (for binocular and trinocular bodies).
7 Close your left eye and focus on the specimen with your right eye using the fine focus knob.
8 Close your right eye and focus on the specimen by rotating the left focusable eyepiece tube.
9 While viewing through the microscope, close the field diaphragm which you have just
installed using the field diaphragm lever. You will see the iris leaves imaged within the field
of view.
10 Using the condenser adjustment focusing knob, focus the condenser until the leaves of the
field diaphragm are in sharp focus.
11 Center the image of the field diaphragm by turning the condenser centering screws.
This is best accomplished by rotating the two screws simultaneously. After centering,
open the field diaphragm until the iris leaves disappear just beyond the field of view.
12 Remove an eyepiece and view the back aperture of the objective. Close the condenser
diaphragm and then reopen the condenser diaphragm leaves until approximately 20% of
the leaves fill your field of view. This will obtain the full resolving power of the microscope.
Replace the eyepiece.
13 When changing to other objectives, the positions of the condenser and field diaphragms
must be reset as in steps 6 – 9 above for optimum resolution and contrast.