Leica CM1860, Instructions For Use Manual, Page: 43 / 108

43
Leica CM1860/CM1860 UV
Daily Use of the Instrument
7
7.2.1 Quick-freeze shelf
The cryochamber is equipped with a quick freeze shelf ( → Fig. 24- 5 ) on which up to 10 specimen stages
with specimens can be stored.
The temperature of the quick freeze shelf is always lower than the cryochamber temperature.
1. Cut the specimen roughly to size.
2. If necessary, enable the Peltier element ( → Fig. 24- 4 ) - it takes approx. 60 seconds until the
maximum cooling capacity is available.
3. Apply a sufficient amount of cryocompound to a specimen disc that is at room temperature or
pre-cooled.
4. Place the specimen on the disc and orient it.
5. Place the specimen disc in one of the holes of the quick freeze shelf and freeze the specimen at
low temperature.
6. Once the specimen is frozen, insert the specimen disc ( → Fig. 25- 3 ) with the specimen into the
specimen head (
→
Fig. 25- 2 ) and start sectioning.
Fig. 24
1
2
3
4
5