Leica BIOSYSTEMS 1404723512, Instructions For Use Manual, Page: 24 / 42

24 Version 2.4, Revision L
Operation
5
Fig. 16
Adjusting the retraction
• In programming mode, press
DISP
to display the specimen
retraction menu.
• Display: "LO".
• Set specimen retraction between 1 and 999 μm via the
-/+ button;
or disable by selecting "0".
• The selected value will be displayed in the FEED window.
• Press
CLR to quit the menu function.
5.4 Adjusting the amplitude
Fig. 17
1
• To obtain excellent sectioning results, the amplitude requires
adjustment according to the specimen type being sectioned.
To this end:
• With a No. 2.5 Allen key loosen the clamping screw ( → Fig. 17- 1 )
and secure the eccentric on the bottom with your finger.
Selectable amplitude positions are, from left to right:
0.2 mm; 0.4 mm; 0.6 mm; 0.8 mm; 1 mm.
• Slide the amplitude clamping screw to the desired amplitude
position and retighten.
Note
When adjusting the amplitude setting, do not remove the clamping screw, simply loosen it.
The instrument is shipped with the amplitude set to 0.6 mm.
5.5 Working with the VT1000 S on a daily basis
Fig. 18
1
2
4
3
• Mount the buffer tray (
→
Fig. 18- 1 ) onto the bolt (
→
Fig. 18- 2 )
inside the cooling bath (
→
Fig. 18- 3 ) .
• Secure the buffer tray by relocating the clamping lever
( → Fig. 18- 4 ) to the right (in the direction of the arrow).
• Via the UP/DOWN toggle switch lower the buffer tray to its
lowest position (indicated by audible signal and red LED).
• Move the toggle switch back to the mid-position - the audible
signal stops.
• If necessary, fill crushed ice into the cooling bath ( → Fig. 18- 3 ) .
• Fill the buffer tray ( → Fig. 18- 1 ) with cooled buffer solution.