LC Packings Probot 161403, User Manual

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Appendix D
User’s Manual Probot
D960R3
D-7
e) Set the ‘Collection control mode’ to ‘External’.
f) Select the Destination tab (FIGURE D-8).
FIGURE D-8 Destination Tab of the Fractionate Task
g) Set the ‘Use special Z –coordinate .. ‘ so that the needle tip rests 2 mm
above the target.
h) Select the ‘Collect peaks only’ option and select the ‘Use vacuum-waste
removal’ option.
i) Save the application and start the program.
In CHROMELEON the following lines have to be added to the program (the
underlined parameters are examples and chromatogram dependent). This is an
example in which the peak start is defined by the slope of the chromatogram and
the peak end by the slope AND the absolute UV signal. Depending on your
chromatographic conditions, other trigger rules may lead to better results.
Probot_Start.On Duration=1.00
Trigger PeakStart UV_VIS_1.Delta > 0.1 , True= 2.00
Pump_Relay_1.State = On
Pump_Relay_1.Duration = 1.00
EndTrigger
Trigger PeakEnd
UV_VIS_1 < 5 and UV_VIS_1.Delta > -0.03 True= 2.00
Pump_Relay_2.State = On
Pump_Relay_2.Duration = 1.00
EndTrigger