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Alpha-1000

SPECTROPHOTOMETER

USER’S MANUAL

V 1.0

Revision 0

Laxco, Inc.

http://www.laxcoinc.com

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Summary of Contents for Alpha-1000

Page 1: ...Alpha 1000 SPECTROPHOTOMETER USER S MANUAL V 1 0 Revision 0 Laxco Inc http www laxcoinc com...

Page 2: ...2...

Page 3: ...s at Different Wavelengths 10 Lamp Changing 11 Lamp Adjustment 12 Absorbance Accuracy and Stray Light Checking 13 Sample Experiments 13 Experiment 1 A Brief Introduction to Beer s Law 13 Experiment 2...

Page 4: ...lay to show absorbency and transmittance Figure 1 illustrates the relationship between these parts Light Source Monochromator Sample Compartment Detector Digital Display Figure 1 Block Diagram for the...

Page 5: ...adiant Energy 2 T at 400 nm Photometric Range 0 T to 125 T 0 3A to 2 0A Photometric Accuracy Better than 2 0 T Data Output USB RS232 Power Requirements 85 240 VAC 50 60 Hz Dimensions 455 L x 340 W x 1...

Page 6: ...uency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations 2 Remove any obstructions or materials that could hinder the flow of air under and around the instrum...

Page 7: ...It is used for connecting to Computer with Application Software RS232 It is used for Firmware upgrading Figure 2 Alpha 1000 Spectrophotometer Sample Compartment Digital Display 0A 100 T Knob Operatio...

Page 8: ...Parts List of this manual for details Follow the steps below to change the Cuvette Sample Holder Open the lid of the Sample Holder and locate the Sample Holder Locking Screw as shown in Figure 4 Use A...

Page 9: ...solution will typically be distilled water but can be the aqueous in water medium that you are using for your experiment The transmittance of this solution is made to read 100 T 0A This process is ca...

Page 10: ...ent this mark is located at the front of the sample compartment Be sure that the test tube has been firmly pressed into the sample compartment and the lid of the sample compartment has been closed 4 A...

Page 11: ...the lamp housing in place refer to the diagram shown below 3 Remove the screw and retain BE CAREFUL THE BULB MAY BE HOT 4 Remove the old bulb and place the leads of the new bulb into the holes in the...

Page 12: ...light bulb is fully inserted into its holder The bulb socket has been permanently mounted in the optimal position by the factory So the rest of steps should not be needed 3 The lamp socket is mounted...

Page 13: ...m s the speed of light in a vacuum often rounded to 3 00 x 10 8 m s and wavelength of light Every substance has a characteristic absorbance of the light spectrum which means that there are wavelength...

Page 14: ...ally zero It is found that absorbance is directly proportional to concentration so that A EBC Where A The absorbance C The concentration in moles liter and B The width of the sample cell or cuvette in...

Page 15: ...sure that the meter display 0 T and 100 T readings are adjusted at each new wavelength Calculations 1 Study the data and identify the points where the peaks are likely to be found Make measurements a...

Page 16: ...f the first standard at this wavelength 6 Repeat step 5 for the other three standards Calculations 1 On a sheet of graph paper label the horizontal axis concentration and mark it in equal intervals fr...

Page 17: ...known and the four standards into the tubes respectively Rinse the pipette after each use 6 Using a graduated cylinder add 2 ml of the alkaline copper titrate solution to each test tube and stir by sw...

Page 18: ...ne hydrochloride solution 10 ml 1M NaC2H3O2 aq sodium acetate solution 10 ml 1 w v 1 10 phenanthroline ortho solution a 100 ml beaker a 50 ml burette five Erlenmeyer flasks a 50 ml graduated cylinder...

Page 19: ...pared unknown solution may not yield an absorbance value that can be properly interpreted from the standard curve Therefore you may have to make adjustments in the concentration of iron in the unknown...

Page 20: ...5 COD Vial Sample Holder A 110 220 Power Cord European plug A 150 115 Holmium Oxide Filter 10 mm x 10 mm x 45 mm requires A 110 103 A 150 116 Didymium Filter 10 mm x 10 mm x 45 mm requires A 110 103 G...

Page 21: ...cannot be set to 00 0 T Sample holder Remove Cuvette Adapter or test tube Sample holder shutter May be stuck open Close shutter Defective electronic component Call an authorized service engineer Inco...

Page 22: ...Defective or dirty detector or defective electronic component Call an authorized service engineer Incorrect readings obtained Insufficient sample volume Fill cuvette with more sample Wrong wavelength...

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