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LAMBDA OMNICOLL Fraction Collector - Sampler

  

Operation MANUAL 2023.01

 

 

 

www.lambda-instruments.com

 

 

23

 

 

Measure the volume of this fraction. This volume corresponds to the pre-set count number. 

From this ratio (volume/count number) you can easily calculate the count number 
corresponding to the desired fraction volume. 

 

Since tubing of 0.5 to 4 mm of internal diameter (with a tubing wall thickness 
~1 mm) can be used in the LAMBDA peristaltic pumps, the fraction volume 
can be selected in a wide range. For example, when tubing with an internal 
diameter of 3 mm is used, then one count corresponds approximately to 
one drop. 

 

3.7  Fraction collection with a time interval in-

between fractions (“high”) 

LAMBDA OMNICOLL fraction collector and sampler could be used for the collection of 
samples with a time interval between consecutive fractions varying between 0.1 min and 
16.6 hours (999.9 min) or 1 min and 166 hours (9999 min).  

This is particularly useful during fermentations and other biological and chemical processes. 
For this application, switch the microprocessor of the control unit to the 

“high”

 

mode

 

Selecting the high mode: 

 

Figure 3.7-1

 Press the 

SET

 button on the 

control unit and connect the plug-in power 
supply to the mains. 

 

Figure 3.7-2

 Use the 

COUNT/TIME button

 to 

select the 

“high”

 mode. 

 

Figure 3.7-3

 Confirm the selection by pressing 

the 

SET

 button (long beep signal). 

 

In the 

“high” mode

 you can successively select the parameters 

TIME, COUNT, PAUSE

 and 

NUMBER

 by pressing the 

COUNT/TIME

 button. 

After selection of the parameter with the 

COUNT/TIME 

button, press the 

SET 

button.  

After a short beep the name of the parameter will appear during one second, followed by last 
used value of the parameter.  

Summary of Contents for OMNICOLL

Page 1: ...LAMBDA OMNICOLL Fraction Collector and Sampler Operation MANUAL LAMBDA OMNICOLL Fraction Collector Sampler...

Page 2: ...onstruction with tubes easily accessible from all sides Multi column attachment for simultaneous fraction collection optional Low voltage power supply battery operation possible for field applications...

Page 3: ...in row 16 3 3 2 Coding Y axis for row distance 17 3 4 Control Panel of OMNICOLL Fraction Collector 17 3 4 1 Selection of linear meander like or row collection 18 3 4 2 Time resolution 0 1 or 1 minute...

Page 4: ...rst tub sheet into the slots on one of the shorter sides of the yellow support Figure 1 1 2 Push the second tub sheet into the slots on another shorter side of the yellow support Figure 1 1 3 Insert t...

Page 5: ...ails The distance from the edge should be the same on the left and right sides The gear wheels of the control unit must engage correctly in the gears of both rails Be sure that nothing will disturb th...

Page 6: ...wn Figure 1 2 3 Slide the O ring on the PTFE tubing Figure 1 2 4 Push the tubing through the tubing guide and make sure a few mm 5mm of PTFE tubing reach out of the tubing guide so that the drops form...

Page 7: ...the first lower part unit and the front side of the second lower part unit Insert the coupling bars Art No 6912 on their place and fix with the four bolts Be aware that you must remove the fixed stop...

Page 8: ...ven more effluents of multiple chromatography columns This multi stream assembly is technically very simple and polyvalent It allows an easy adaptation of various experimental needs directly by the us...

Page 9: ...t a convenient height above the tubes is obtained Fix the tubing guide to the holder using the corresponding socket key hex key Figure 1 4 1 10 Place the tubing guide holder at the desired fraction po...

Page 10: ...le up to 8 channels Examples of customized multi stream fraction collector LAMBDA OMNICOLL Version 2022 Figure 1 4 2 1 Front side of the customized multi channel fraction collector LAMBDA OMNICOLL for...

Page 11: ...you need to assemble the moving arm holder Figure 1 4 2 6 The holder is fixed with two screws using a hexagonal key Figure 1 4 2 7 The moving arm holder attaches to the black rectangle attached to th...

Page 12: ...com 11 Figure 1 4 2 11 The tube guide is mounted on a moving arm in accordance with the selected position For each stream one tube guide art no 6950 is needed Figure 1 4 2 12 The tube guide is fixed...

Page 13: ...rotated Slightly loosen the nut C so that the PTFE tubing D can move freely inside the tubing guide B Screw the drop counter E on the lower end of the tubing guide B Tighten the tubing guide holder sc...

Page 14: ...nsing tubing is about 1 cm above the tubes or recipients Place the tube rack on the fixing mat Figure 1 1 8 so that the first tube is just under the tip of the tubing and the edge of the rack is paral...

Page 15: ...e fraction positioning Figure 3 1 2 The fraction positions are indicated by white lines drawn on the black magnetic coding band as shown The white lines should be about 2 mm broad and full length Thes...

Page 16: ...etic coding bands programmed with white lines for the desired tube racks are provided with the OMNICOLL Fraction collector Figure 3 2 1 Place the longer and narrower coding band Y axis on the left inn...

Page 17: ...lines correctly on the pre marked places Reserve approximately 2cm of the coding band for the beginning and end position signals Figure 3 3 3 Mark the beginning and end signals on the lower and upper...

Page 18: ...ing band on the left internal side of the metal frame just under the gear bar If the fraction collection should be stopped at the last row then make the last line broader approximately 1 cm thick This...

Page 19: ...he collecting arm does not move within the row The collector moves only from row to row This collection mode is mainly used in the simultaneous multi channel fraction collection mode Figure 3 4 1 Pres...

Page 20: ...ion is of approx 1 mm 3 4 2 Time resolution 0 1 or 1 minute The time resolution of the OMNICOLL fraction collector can be selected in the following way Figure 3 4 6 Press the button COUNT TIME while c...

Page 21: ...n of motor are counted In the second setting sixty drops or impulsions give just one signal Figure 3 4 10 Press the button START while plugging the power supply into the mains Figure 3 4 11 div and th...

Page 22: ...tions of precise volume from 0 05 to 500 ml or 0 6 to 30 litres per fraction The heart of these pumps is a stepping motor or BLDC motor controlled by a generator of electric impulses microprocessor Af...

Page 23: ...ong connection because the connectors will not fit to the wrong sockets Figure 3 6 1 Connect the peristaltic pump remote control cable to the communication module box Figure 3 6 2 With the COUNT TIME...

Page 24: ...sed for the collection of samples with a time interval between consecutive fractions varying between 0 1 min and 16 6 hours 999 9 min or 1 min and 166 hours 9999 min This is particularly useful during...

Page 25: ...ction collection is started by pressing the START button The collector will take the number of samples as programmed under the NUMBER setting and will stop for the time programmed under the PAUSE sett...

Page 26: ...alarm The fraction collector can take one or several samples after detection of a signal 3 to 12 V or 12 to 30V with a 3300 ohm resistor switched in series The LAMBDA peristaltic pump will be activat...

Page 27: ...cording to your needs This makes it possible to use even largest containers for taking fractions For such applications a modified tubing holder can also be made On demand we can also supply an adapter...

Page 28: ...Collector takes a 1 to 999 fractions after a single external voltage pulse of 3 12 V or 12 30 V with a 3300 ohm resistor Capacity Any tube rack or container type with a surface smaller than 45x31cm U...

Page 29: ...10 000 ml hr programmable 6910 Remote control cable for fraction collector analog 6910 rs RS 232 connection cable 6911 Communication module for pump switching and RS 232 connection 6912 Set for keepin...

Page 30: ...for Watson Marlow 205U peristaltic pump 4810 LongerBT Remote control cable for Longer BT 100 1 peristaltic pump 4810 WM PF6 Remote control cable for Watson Marlow Flexicon PF6 pump drive peristaltic p...

Page 31: ...must be returned with a complete description of the defect or problem In order to send back the equipment for repair you will need a returns authorization number from LAMBDA The customer will send th...

Page 32: ...vates remote control of the collector front panel deactivated ss mm g qs c activates local mode front panel activated ss mm s qs c stop ss mm f qs c step forward ss mm b qs c step back ss mm w qs c st...

Page 33: ...time xxxx in minute steps resp number of pulses or fractions collector is in stand by mode mm ss R xxx x qs c time xxx x in 0 1 minute steps collector is in operation mm ss R xxxx qs c time xxxx in mi...

Page 34: ...s of the fraction collector To change the address press the buttons under the display until the desired number is obtained Confirm your choice by pressing the button SET 11 3 RS connection scheme The...

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