Depending upon use, we recommend checking of calibra on
every six months. However this can be adjusted to individual
requirements.
6.3 PROCEDURE TO CHECK CALIBRATION
The pipe e is checked at maximum volume, at 50 % of maximum
volume and at minimum or 10% of maximum volume, whichever is
higher.
•
A new p is first pre-we ed 3-5 mes and a series of ten
pipe ng is done at each volume.
•
Use of forward pipe ng technique is recommended.
- 10 -
6.1 DEVICE REQUIREMENTS AND TEST CONDITIONS
An analy cal balance must be used. The balance selec on depends
upon selected model of the pipe e and sensi vity of balance
reading.
Test liquid: Water, dis lled or de-ionized, grade 3 water conforming
ISO3696 Calibra on should be carried out in a dra -free room at a
constant (±0.5°C temperature of water, pipe e and air between
15°C to 30°C.
The rela ve humidity must be above 50% especially with volumes
under 50 µl, the air humidity should be as high as possible to reduce
the effect of evapora on loss.
Special accessories for analy cal balance, such as the evapora on
trap are recommended for the calibra on of volumes under 50 µl.
6.2 CALIBRATION ADJUSTMENT
1.
Calibra on adjustment is done with the calibra on tool
provided along with pipe e.
2.
Rotate the volume locking lever to the “lock” posi on so the
volume se ng mechanism is locked and able to turn the
calibra on screw.
3.
Remove volume adjustment knob by pulling it upwards.
4.
Place the calibra on tool into the calibra on grooves.
5.
Rotate the calibra on tool counter clockwise to increase and
clockwise to decrease the volume.
6.
A er adjustment, check the calibra on according to the
instruc on in point number 6.3
7.
Once within permissible error range, remove the calibra on
tool from pipe e and place volume adjustment knob in its
original posi on.
100
1000
µl
