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Sulphur BioDenitrator  

S1502 / S3002 / S4002 

 
 

 

The Korallin S-series Sulphur BioDenitrator is an efficient nitrate remover that does not require 
the use of an ORP controller or the feeding of the denitrifying bacteria. Thanks to its highly 
efficient recirculating design, nitrates are reduced quickly and minimal adjustments are required 
to keep the filter operating smoothly. Used in conjunction with the SupraPure sulphur media, the  
pure coral-skeleton media adds the benefit of buffering the effluent solution to keep the pH stable 
in the Aquarium. 
 

Instructions:

 Thoroughly rinse the BioDenitrator with freshwater. Fill 50% (~5”) the reaction 

chamber with SupraPure sulphur media (99.9% pure). Next, fill the reaction chamber with 2-3” of 
coral-skeleton media. Make sure to leave a minimum of 3” between the top of the media and the 
top of the reaction chamber so that the inlet tube does not become clogged during operation and 
that you are able to see any air bubbles forming or trapped at the top of the BioDenitrator.  For 
freshwater tanks with lower pH (pH 7.0 or lower), the coral-skeleton media is not required.  
 
Once the media has been placed inside the reactor chamber, close off the lid and tighten all 
screws. Secure the Eheim pump to the top of the BioDenitrator with the Nylon screw and nut 
provided. Attach the “Einlauf” (inlet) and “Ausgang” (outlet) assemblies to the top of lid and to the 
Eheim pump. Using the tubing provided, connect both the “Einlauf” (inlet) and the “Ausgang” 
(outlet) to the tank. Make sure that the tubing is fitted tightly to prevent leaks! With both the 
“Einlauf” and “Ausgang” lines submersed under water, open the "Entlüftung" and begin to 
manually siphon water up through the "Entlüftung" tube to fill the chamber.  The BioDenitrator 
must be installed lower than the tank’s, or sump’s, water level (for installations where the 
BioDenitrator is higher than the water level in the tank or the sump, a separate feeding pump is 
required).   
 
Once the reaction chamber is completely filled with water, close the "Entlüftung" valve. Elevate 
the “Ausgang” line so that it is about 1” above the water line: this will allow you to monitor the drip 
rate more easily. Open the “Ausgang” valve fully and start the Eheim pump. Let the BioDenitrator 
run for 2-3 hours then throttle back the “Ausgang” valve to about 2 drops per second (effluent drip 
rate). Allow the BioDenitrator to run at this rate for one week: the effluent drip rate can fall to 
about 1 drop per second without causing any adverse effects.  
 
After one week, reduce the effluent drip rate to 1 drop per every 2 seconds. Now, measure the 
NO2 (nitrite) level with a test kit. If the NO2 (nitrite) level is 0 ppm, change the effluent drip rate to 
1 drop per second. Allow the BioDenitrator to run for 24 hours then test the NO2 level and NO3 
(nitrate) level: both in the aquarium and of the effluent. If the NO2 level remains at “0” and the 
nitrate level of the effluent is lower than the nitrate level of your aquarium water, the BioDenitrator 
has begun reducing nitrate (NO3).  
 
* If the nitrite (NO2) level is higher than 0 ppm, there is no need to test the nitrate (NO3) level as 
the result would in incorrect. 
 
It will take 4-6 weeks for denitrifying bacteria to fully colonize the media in the BioDenitrator. After 
this time, you can begin to tune BioDenitrator by adjusting the effluent drip rate to obtain the 

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