Knauer BlueShadow Detector 40D Instructions Manual Download Page 1

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Detector 40D

Instructions

Document no. V7647

HPLC

Summary of Contents for BlueShadow Detector 40D

Page 1: ...Detector 40D Instructions Document no V7647 HPLC HPLC...

Page 2: ...809727 111 workdays 9 17h CET Fax 49 30 8015010 E mail support knauer net KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Germany Phone 49 30 809727 0 Fax 49 30 8015010 Internet www...

Page 3: ...oreseeable misuse 3 2 2 User qualification 3 2 3 Operator responsibility 4 2 4 Personal safety equipment 4 2 5 Working with solvents 5 2 5 1 General requirements 5 2 5 2 Contamination by health threat...

Page 4: ...th LAN 18 4 6 4 Configuring the router 19 4 6 5 Integrating LAN into the company network 20 4 6 6 Controlling several systems separately in LAN 20 4 7 Remote control 21 4 7 1 Connector assignment 21 4...

Page 5: ...6 Functionality tests 45 6 1 Installation Qualification IQ 45 6 2 Operation Qualification OQ 45 7 Troubleshooting 46 7 1 LAN 46 7 2 Possible problems and solutions 47 7 3 System messages 48 8 Mainten...

Page 6: ...outdated PETP 61 Polyimide Vespel 62 Ethylene tetrafluorethylene copolymer ETFC Tefzel 62 Perfluorethylenpropylen Copolymer FEP Perfluoralkoxy Polymer PFA 62 Polyoxymethylene POM POM H TF 62 Polypheny...

Page 7: ...these and other instructions from the KNAUER web site www knauer net library 1 2 Signal words Possible dangers related to the device are distinguished in personal and material damages Symbol Meaning D...

Page 8: ...e In case you notice any damage inform the Technical Support and the shipping company within three workdays 1 4 3 Warranty conditions For information on warranty please refer to our general terms and...

Page 9: ...t guarantee the functionality and safety of the device Operation in potentially explosive areas without special and additio nal explosion protection Contact the KNAUER Customer Support for more inform...

Page 10: ...learly regulate and specify responsibilities for installation operation troubleshooting maintenance and cleaning and set clear rules Ensure that all personnel who work with the device have read and un...

Page 11: ...s could get onto hot surfaces in the interior Degas solvents before use and observe their purity 2 5 2 Contamination by health threatening solvents Contamination with toxic infectious or radioactive s...

Page 12: ...wet rooms 2 7 Maintenance care and repair Avoiding electric shock Before performing any maintenance and ser vice work disconnect the device from the power supply Tools Use only tools recommended or pr...

Page 13: ...quest form and decontamination report Devices which are shipped without the completed document Service request form and decontamination report will not be repaired If you would like to return a device...

Page 14: ...able independent of the selected wavelength Automatic recording and storage of the device specific characteristics that are important for GLP Good Laboratory Practice and OQ Opera tion Qualification o...

Page 15: ...chromatography software at the worksta tion or the keypad Legend 1Status LED 2Keypad 3Flow cell with holder Fig 1 Front view 3 3 2 Rear view The rear of the device contains the mains power connection...

Page 16: ...stem device or components can occur A device marked with CE fulfills the product spe cific requirements of European directives This is confirmed in a Declaration of Conformity Testing seals in Canada...

Page 17: ...tray as these parts are loosely attached to the device Keep the included packing list for repeat orders Keep the original packaging for safe storage or transportation 4 2 Ambient conditions 4 2 1 Oper...

Page 18: ...riefly when the device is switched on Main connection The electrical power supply at the operation site must be connected directly to the nearest main power connection Grounding The connectors for the...

Page 19: ...ocess Figure 1 Unscrew the knurled head screws 3of the cover plate 4 Hold the flow cell securely with your hand during this procedure 2 Pull out the slide with the flow cell 5towards the front 3 Remov...

Page 20: ...roceed as fol lows Procedure Process Figure 1 Remove the threaded ring 1using a hexagonal spanner 2 Remove stainless steel cover 2and the PEEK spacer 3 3 Insert the stainless steel cover again and car...

Page 21: ...mm Please use a clean cloth and avoid touching the light guide with your fingers 4 Push the light guide together with the pressure bushing back into the cell 5 Insert the PEEK spacer 3fol lowed by the...

Page 22: ...ure 1 Push the capillary 2through the fitting 1 2 Push the clamping ring 3 onto capillary Fig 6 Capillary fitting 3 Manually screw together the capillary 5and the flow cell 4 Tighten the fitting with...

Page 23: ...n assigned by IPv4 IPv6 is not supported This chapter describes how to set up a chromatography system in a local area network LAN and how a network administrator can integrate this LAN into your compa...

Page 24: ...lick on the button Properties 6 Check the settings in the tab General The correct settings for the DHCP client are a Obtain an IP address automatically b Obtain DNS server address automatically 7 Clic...

Page 25: ...ays switched on in the same order Set the router properties see section 4 6 4 4 6 4 Configuring the router The router is preset at the factory Information about address user name and password is noted...

Page 26: ...parately in LAN Devices connected to a LAN communicate through ports which are part of the IP address If more than one chromatography systems are connec ted to the same LAN and you plan on controlling...

Page 27: ...2LAN connector 3Pin header 4RS 232 port Fig 10 Connector pin assignment on rear side Signal Explanation EV 1 Event 1 Relay contact The contact is on a floating basis Its setting depends on the settin...

Page 28: ...ctronic trigger is send through the LAN Autozero Low active Secure switching threshold at least 10 mA A signal short circuit to ground sets the measuring sig nal to zero 24V Event controlled switching...

Page 29: ...switched on device causes a short circuit Turn o the device before connecting cables Pull the power plug Electronic defect Electrostatic discharge can destroy the electronics Wear a protective bracel...

Page 30: ...tor sends measuring signals from the detector Non bipolar 1 channel 0 to 5 V DAC 20 bit Scalable Adjustable to offset 4 10 Switching on The device is switched on by the power key on the back of the de...

Page 31: ...he capillaries have been connected 5 2 Software control There are several options for controlling the device With keypad With chromatography software 5 2 1 Controlling with display and keypad The disp...

Page 32: ...pad 5 2 3 Status LED The status of the device is indicated by an LED on the front side The color of the LED shows the current status LED Status Green Device is ready idle status Green flashing Device...

Page 33: ...on Setting current absor bance values to zero Menu button Select main menu Return to one menu further up in the menu structure Confirmation button Confirm selection Entering sub menu Activation of num...

Page 34: ...irmation button or use the vertical arrow keys 3 Confirm entry by using the horizontal arrow keys Note One spectral edge filter to suppress the second diffraction order is automatically activated if t...

Page 35: ...GLP LAMPS GLP DEVICE PROGRAMS and DEVICE LINKS use the menu button and the horizontal arrow keys Navigate through main menu 1 Press to access the menu 2 Navigate between menu points by using horizonta...

Page 36: ...30 Operation BlueShadow Detector 40D Instructions V7647 5 4 2 Menu structure overview Fig 15 Menu structure overview...

Page 37: ...n of the detector The connections are on the rear of the device Serial interface RS232 115200 RS232 38400 RS232 19200 RS232 9600 LAN DHCP Automatic network configuration LAN manual Manual net work con...

Page 38: ...measured for signal and reference channel P sig ref provides the ab solute light intensity of signal and reference channel at the UV maximum measured after a calibration The values are independent fr...

Page 39: ...hreshold in the Fraction level is exceeded the event output selected in the Fraction port line is switched on and remains active until the signal drops below the threshold value The threshold always r...

Page 40: ...gth with a scaling factor of 100 nm V This wavelength WL can then be adjusted Thus a second calibration point is generated which modifies the scaling factor Note Before operating this configuration ch...

Page 41: ...u contains no editable parameters Menu Explanation Figure DETECTOR GLP Serial number Detector serial number Operating time Detector operating time Motor Working Grating motor operating move ment time...

Page 42: ...ps for each program The following parameters can be defined for individual program steps Start time Wavelength Events Navigate through Program menu 1 Select the Device Programs menu 2 Use the horizont...

Page 43: ...ed to monitor absorbance at 280 nm for the first 10 minute step and at 420 nm during the next step which has no time limit since it is the final step in this example Additionally event 1 is ena bled d...

Page 44: ...H and press the up ward key to change the status from Hold H to Run R program starts running 5 You can again go back from Run R to hold H by pressing the downward key with the cur sor at the status s...

Page 45: ...e the vertical arrow keys 5 6 4 Deleting a program line Process Figure 1 Select the Device Programs menu 2 Select the program number you wish to change 3 Navigate to the to be deleted program line 4 C...

Page 46: ...d the program number you wish to start by navigating via the horizontal arrow keys and editing via the numeric pad after pressing the confirmation key or using the vertical arrow keys 4 To run the wak...

Page 47: ...he numeric pad Confirm the selected link by using the confirmation key 3 You can view the progress of the currently run ning link in the third link display 4 After selecting a link use the horizontal...

Page 48: ...ursor to position P P Program in the display and enter the program number you wish to run 5 Move the cursor to position R R Repeat and edit the value number of repetitions for the specified program 6...

Page 49: ...ia the upward ar row key The detector status will switch to R Run mode 6 The link will start running the programs line by line 7 Use the downward arrow key with the cur sor on the detector status to p...

Page 50: ...k number you wish to change 3 Navigate to the to be deleted link line e g line 02 4 Change the line number to 00 5 You will be asked whether you want to delete the link line which was active before en...

Page 51: ...sion from our website 6 2 Operation Qualification OQ The Operation Qualification includes an extensive functionality test according to KNAUER standard OQ documents The Operation Qualification is a sta...

Page 52: ...computer 2 Check the router settings Is the router set to DCHP server Is the IP address range sufficient for all the connected devices 3 Check all connections Are the patch cable connected to the LAN...

Page 53: ...ent incursion from interfering light or an electronics error Insert the test cell Inspect the calibration with a weak absorbing eluent Baseline noise Inspect the flow cell assembly Fasten the knurled...

Page 54: ...delete program Cannot edit pro gram from the run ning link First pause link then edit data using the keypad Cannot initialize LAN Check cables and connections in local area net work Cannot operate an...

Page 55: ...e program Check the detector Check the number of pro gram lines A maximum of 100 program lines are possible P Program does not exist Create a program Program is running Quit program or wait until prog...

Page 56: ...BlueShadow Detector 40D Instructions V7647 System message Too many lines in program Check the number of program lines A maximum of 100 program lines are possible W Wrong Line number Change the entry i...

Page 57: ...tomer Support 8 1 Maintenance contract The following maintenance work on the device may only be performed by KNAUER or a company authorized by KNAUER and is covered by a sepa rate maintenance contract...

Page 58: ...Process Figure 1 Remove capillaries at the inlet 1and outlet 2of the flow cell 2 Unscrew the knurled head screws 3of the cover plate 4 Hold the flow cell securely with your hand during this procedure...

Page 59: ...e flow cell Flow cell is removed from the detector Waste tube is connected to the flow cell The following solvents are recommended for rinsing Diluted HCl 1 mol l 1 ml l NaOH aq Ethanol Acetone Syring...

Page 60: ...clean surface remove the compression part 2 3 The lens 3 is protected by a seal ring 4 This must be renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or w...

Page 61: ...g 1 2 Remove the cover 2 and the spacer 3 not part of all flow cells 3 Using tweezers pull out the pressure bushing 4and the light guide 5 4 Carefully push the light guide out of the holder and strip...

Page 62: ...uld falsify the measurement Handle the fiber optics with care and avoid impacts Move the fiber optics carefully without using pressure or bending it Detector is switched off Cap fittings for fiber opt...

Page 63: ...sport or storage If you want to return your device to KNAUER for repairs enclose the Service Request Form which can be downloaded from our website For a secure transport note the weight and dimensions...

Page 64: ...r classifies to category 8 and 9 which among others comprises laboratory equipment All distributors and importers are responsible for the disposal of old devices as defined by the WEEE directive End u...

Page 65: ...at H line FWHM Wavelength accu racy 2 5 nm Wavelength preci sion 0 3 nm ASTM E1657 98 Noise 1 5 10 5 AU at 254 nm 2 0 x 10 5 AU at 254 nm fiber optics version ASTM E1657 98 Drift 3 0 10 4 AU h at 254...

Page 66: ...operating hours number of lamp ignitions Display Matrix keypad 11 3 General Power supply Power input 100 240 V Output 50 60 Hz Maximum power consumption 75 Watt Protection type IP 20 Dimensions W H D...

Page 67: ...herwise Please note that higher temperatures can signifi cantly affect the stability of different materials 12 2 Plastics Polyetheretherketone PEEK PEEK is a durable and resistant plastic and next to...

Page 68: ...ene POM POM H TF POM is a semi crystalline high molecular thermoplastic material which stands out due to its high stiffness low friction value and thermic stability It can even substitute metal in man...

Page 69: ...terized by a high hardness a low coefficient of fric tion and thus low wear In addition it is highly biocompatible DLC is inert against all acids alkalis and solvents commonly used in HPLC Ceramic Cer...

Page 70: ...e used or with a mixture of higher compatibility They are inert against almost all solvents Exceptions are biological appli cations which are metal ion sensible and applications with extreme cor rosiv...

Page 71: ...Appendix Installation Qualification IQ...

Page 72: ...cated by n a not applicable in the table Larger sections that are not used have to be crossed out diagonal line through them marked with n a and date DD MM YYYY and signed All required documents have...

Page 73: ...transport damage No transport damage is observed 3 Check the scope of delivery The scope of delivery is complete 4 Connect the device to the power supply The device is connected 5 Connect all loose p...

Page 74: ...o be signed by a person who is authorized by the customer s head of laboratory The scope of the IQ meets the customer s requirements The results of the IQ any changes made as well as the IQ process ha...

Page 75: ...UER instructions online www knauer net library KNAUER Wissenschaftliche Ger te GmbH Hegauer Weg 38 14163 Berlin Phone Fax E Mail Internet 49 30 809727 0 49 30 8015010 info knauer net www knauer net KN...

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