17
Startup
KNAUER
Degasser 20DG User Manual V7632A, Version 1.1
Startup
Switching on the Degasser
Requirements
All lines have been connected correctly.
Unused degasser chambers have been sealed off with filler
caps.
Procedure
1. Connect the power supply unit of the device to the mains.
During the start procedure, the INIT LED lights up for approx.
30 seconds. After the specified vacuum value has been
reached, the INIT LED goes out and the READY LED lights up.
The device is now ready for operation.
2. Switch on the HPLC pump.
3. To perform a solvent change, first flush with a liquid in which
both solvents are soluble. We recommend 2-propanol, for
example. Due to the low chamber volume, only small
amounts of the solvents are needed for flushing.
4. When you stop the HPLC pump, also switch off the degasser.
Flushing the Degasser
Flush the degasser and the connecting tubes with the appropri-
ate solvent prior to use.
Note
Prior to changing the solvent in the degasser, flush with a liquid
in which both solvents are soluble.
Flushing the Degasser with a Syringe
Usually, 2 ml solvent is adequate for flushing.
1. Connect the solvent tube to the outlet of the degassing
chamber.
2. Connect the syringe to the solvent tube.
3. With the syringe, extract solvent through the degasser until
there are no air bubbles in the solution any more.
4. Repeat step 1–4 for the degassing chambers that are to be
used.
Flushing the Degasser with the Pump
CAUTION! Very high pressures damage the
degasser membrane. The membrane can with-
stand a maximum pressure of 7 bar.
Do not connect the degasser to the pump outlet.
1. Configure the pump: Flow rate 2 ml/min.
2. Start the pump and flush for 1 to 2 minutes.
