14
Initial Startup
DG 2.1S user manual V6880, version
3.3
5. Switch on the HPLC pump.
When you switch off the HPLC pump, also switch off the degasser.
Purging
Flush the degasser and the connecting tubes with the appropriate solvent prior
to use. Thanks to the low chamber volume, only small amounts of the solvents
are needed for flushing.
Prerequisite
The degasser is connected to the pump.
Note:
Prior to a solvent change in the degasser, purge with a liquid in which
both solvents are soluble.
Procedure
1. Configure the pump: Flow rate 2 ml/min.
2. Start the pump and flush for 1 to 2 minutes.
Leak Test
The vacuum is continuously checked by means of a built-in microprocessor and
the pump output adjusted. Thus it is possible to detect leaks.
Procedure in the event of a detected leak
If a leak is detected, the microprocessor of the degasser automatically
increases the pump speed to maintain the vacuum. If the vacuum still falls
below the target value, the yellow LED flashes.
If it is not possible to achieve the required vacuum within 30 minutes by
means of the increased pump output, the LED lights up yellow at the degas-
ser to indicate that there is possibly a leak in the system.
The degasser will be switched off automatically.
Switching Off the Degasser
Here a differentiation is made between short-term and long-term switch-off.
Note:
To separate the degasser from the mains, remove the switched-mode
power supply from the rear of the device.
Short-Term Switch-Off
Note:
Damage to the degasser that results from using buffer-containing sol-
vents are not covered by the warranty.
Procedure
1. Remove critical solvent from the degassing chambers and other system com-
ponents by purging with for example isopropanol (2-propanol) .
2. Subsequently purge with water.
