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OBL-14-15-BA-e-1610

5.9 Phase contrast unit

Simple phase-contrast unit

This consists of a simple PH condenser, a PH objective with a specific magnification
(10x, 20x, 40x or 100x), a PH slider, which is adapted to the lens being used, a
centring telescope and a green filter.

The standard outfit of the models of the series OBL-14 and OBL-15 always includes
a 10x and 40x phase contrast unit.

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Summary of Contents for OBL 145

Page 1: ...gelei 1 D 72336 Balingen E Mail info kern sohn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Transmitted light phase contrast microscope KERN OBL 14 OBL...

Page 2: ......

Page 3: ...4 3 Eyepieces 10 4 4 Colour filter 10 4 5 PH condenser PH slide 11 5 Operation 11 5 1 Getting started 11 5 2 Pre focussing 12 5 3 Adjusting the interpupillary distance 13 5 4 Dioptre adjustment 13 5...

Page 4: ...an additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The inf...

Page 5: ...hich are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 85 should not be exceeded The device should always...

Page 6: ...as these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However orga...

Page 7: ...OBL 14 15 BA e 1610 6 2 Nomenclature...

Page 8: ...7 OBL 14 15 BA e 1610 Rear view Adjusting ring torque Carrying handle Main switch Power connection Coarse adjustment knob Fine adjustment knob Fuse Condenser Focus dial Adjusting ring torque...

Page 9: ...ocular 6V 20W Halogen Transmitted light OBL 155 Infinity Trinocular 6V 20W Halogen Transmitted light Eyepieces WF 10x 20 mm Objectives 4x PH10x PH40x 100x Product dimensions 395x200x380 mm Packaging d...

Page 10: ...9 OBL 14 15 BA e 1610...

Page 11: ...n such a way that if you turn the nosepiece clockwise the objective with the next higher magnification appears When the objectives need to be dismounted you should always make sure that you do not tou...

Page 12: ...erted into the appropriate point on the front side of the condenser For further information on this please see 5 9 Phase contrast unit 5 Operation 5 1 Getting started The very first step is to establi...

Page 13: ...s soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left...

Page 14: ...ircular image then the interpupillary distance is set correctly 5 4 Dioptre adjustment The eye strengths of each eye of the microscope user can often be slightly different which in daily life has no c...

Page 15: ...l objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam p...

Page 16: ...ion However this is regarded as Fixed Koehler since the condenser is pre centred When adjusting the Koehler lighting for the first time you must first select the lowest possible objective magnificatio...

Page 17: ...e illustration on the right The diameter of the aperture diaphragm which is then visible should make up approximately 2 3 of the pupil diameter If the eyepiece should be removed for checking then plea...

Page 18: ...lasses symbol on the side They are also marked in the item description by an additional H example HSWF 10x 23 mm When fitting the eye cups make sure that the dioptre setting is not moved We would ther...

Page 19: ...slide 2 Lower the specimen stage and position the 100x objective in the beam path 3 Bring the specimen stage or object slide to the objective very slowly until there is slight contact 4 Observe the ob...

Page 20: ...simple PH condenser a PH objective with a specific magnification 10x 20x 40x or 100x a PH slider which is adapted to the lens being used a centring telescope and a green filter The standard outfit of...

Page 21: ...the ring in the slider however can be Before starting the phase contrast application you have to check if this alignment is given For this you must install the centring telescope to the tube connecto...

Page 22: ...der is on the underside of the device It can be opened by undoing the screws on the holder see left illustration We recommend that here you should also test again to check that heat is no longer being...

Page 23: ...ead then a suitable adapter must be fitted In general there are two C mount adapters available for this 1x and 0 47x magnification see Chapter 3 Features After fitting one of these adapters it can be...

Page 24: ...tly centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is betwe...

Page 25: ...s Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path Th...

Page 26: ...ly materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in forc...

Page 27: ..._______________________________________________________ __________________________________________________________ __________________________________________________________ __________________________...

Page 28: ...27 OBL 14 15 BA e 1610...

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