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OBF-1_OBL-1-BA-e-1510 

 

2 

 

 

 

           

GB 

KERN  OBF-1, OBL-1 

Version 1.0  01/2015 

User instructions 
Transmitted light microscope 

 

 

 
Table of contents 
 

1

 

Before use .................................................................................... 3

 

1.1

 

General notes ............................................................................................................................ 3

 

1.2

 

Notes on the electrical system ................................................................................................ 3

 

1.3

 

Storage ...................................................................................................................................... 4

 

1.4

 

Maintenance and cleaning ....................................................................................................... 5

 

2

 

Nomenclature ............................................................................... 6

 

3

 

Technical data / Features ............................................................ 8

 

4

 

Assembly .................................................................................... 13

 

4.1

 

Microscope head .................................................................................................................... 13

 

4.2

 

Objectives ............................................................................................................................... 13

 

4.3

 

Eyepieces ................................................................................................................................ 13

 

4.4

 

Colour filter ............................................................................................................................. 13

 

4.5

 

Condenser ............................................................................................................................... 14

 

5

 

Operation .................................................................................... 14

 

5.1

 

Getting started ........................................................................................................................ 14

 

5.2

 

(Pre-) focussing ...................................................................................................................... 15

 

5.3

 

Adjusting the interpupillary distance ................................................................................... 16

 

5.4

 

Dioptre adjustment ................................................................................................................. 16

 

5.5

 

Adjusting the magnification .................................................................................................. 17

 

5.6

 

Adjusting the Koehler illumination ....................................................................................... 18

 

5.7

 

Using eye cups ....................................................................................................................... 20

 

5.8

 

Using oil immersion objectives ............................................................................................ 21

 

6

 

Changing the bulb ..................................................................... 22

 

7

 

Changing the fuse ...................................................................... 23

 

8

 

Using optional accessories ....................................................... 23

 

8.1

 

Polarisation unit ..................................................................................................................... 23

 

8.2

 

Camera connection ................................................................................................................ 24

 

8.3

 

Dark field units ........................................................................................................................ 24

 

8.4

 

Phase-contrast unit ................................................................................................................ 25

 

8.5

 

Fluorescence reflected light unit .......................................................................................... 27

 

9

 

Trouble shooting ........................................................................ 38

 

10

 

Service ........................................................................................ 40

 

11

 

Disposal ...................................................................................... 40

 

12

 

Further information .................................................................... 40

 

 

 

Summary of Contents for OBF 121

Page 1: ...Mail info kern sohn com Tel 49 0 7433 9933 0 Fax 49 0 7433 9933 149 Internet www kern sohn com User instructions Transmitted light laboratory microscope KERN OBF 1 OBL 1 OBF 121 OBF 122 OBF 123 OBF 131 OBF 132 OBF 133 OBL 125 OBL 127 OBL 135 OBL 137 Version 1 0 01 2015 ...

Page 2: ......

Page 3: ...13 4 5 Condenser 14 5 Operation 14 5 1 Getting started 14 5 2 Pre focussing 15 5 3 Adjusting the interpupillary distance 16 5 4 Dioptre adjustment 16 5 5 Adjusting the magnification 17 5 6 Adjusting the Koehler illumination 18 5 7 Using eye cups 20 5 8 Using oil immersion objectives 21 6 Changing the bulb 22 7 Changing the fuse 23 8 Using optional accessories 23 8 1 Polarisation unit 23 8 2 Camera...

Page 4: ...is an additional risk of triggering an electric shock 1 2 Notes on the electrical system Before connecting to a mains power supply you must make sure that you are using the correct input voltage The information to select the correct mains cable is located on the device on the rear of the product directly above the connection socket You must comply with this information If you do not comply with th...

Page 5: ... which are too high or too low vibrations dust or a high level of humidity The ideal temperature range is between 0 and 40 C and a relative humidity of 85 should not be exceeded The device should always be located on a rigid smooth horizontal surface When the microscope is not being used you should cover it with the enclosed dust protective cover When doing this the power supply is stopped by swit...

Page 6: ...l as these are highly flammable substances You must therefore keep it away from naked flames and electrical devices which can be switched on and off and only use it in well ventilated rooms However organic solutions of this type should not be used to clean other components of the device This could lead to damage to the paint finish To do this it is sufficient to use a neutral cleaning product You ...

Page 7: ...cope head Tube Adjusting screw Head Nosepiece Objective Object holder Specimen stage Aperture adjustment Condenser Condenser Field lens with Field diaphragm Control dial X Y axis Specimen stage Fine adjustment knob Dimmer Coarse adjustment knob Lens barrel ...

Page 8: ...7 OBF 1_OBL 1 BA e 1510 Rear view Adjusting ring torque Carrying handle Main switch Power connection Coarse adjustment knob Fine adjustment knob Fuse Condenser Focus dial Adjusting ring torque ...

Page 9: ...0W Halogen Transmitted light OBF 133 Finite Trinocular 3W LED Transmitted light OBL 125 Infinity Binocular 6V 20W Halogen Transmitted light OBL 127 Infinity Binocular 3W LED Transmitted light OBL 135 Infinity Trinocular 6V 20W Halogen Transmitted light OBL 137 Infinity Trinocular 3W LED Transmitted light Eyepieces OBF 1 Eyepieces OBL 1 WF 10x Ø 18 mm WF 10x Ø 20 mm Objectives 4x 10x 40x 100x Produ...

Page 10: ...9 OBF 1_OBL 1 BA e 1510 ...

Page 11: ...OBF 1_OBL 1 BA e 1510 10 ...

Page 12: ...11 OBF 1_OBL 1 BA e 1510 ...

Page 13: ...OBF 1_OBL 1 BA e 1510 12 ...

Page 14: ...her magnification appears When the objectives need to be dismounted you should always make sure that you do not touch the lenses with your bare fingers and that no dust enters the apertures For objectives which are marked OIL you must use an immersion oil with the lowest level of inherent fluorescence 4 3 Eyepieces You must always use eyepieces with the same magnification for both eyes These are s...

Page 15: ...ry first step is to establish a power connection using the mains plug You should first adjust the dimmer to a low level so that when you look through the eyepiece for the first time your eyes are not immediately subject to a high level of light You can now switch on the lighting using the main switch The next step is to place an object holder with the sample on the angle table To do this the cover...

Page 16: ... As soon as an image is recognisable no matter how sharp then you should only adjust the focus using the fine adjustment knob Adjusting the torque of the coarse and fine adjustment knob Next to the left adjustment wheel for the coarse and fine adjustment knob there is a ring which you can use to alter the torque of these wheels Turning it in a clockwise direction reduces the torque and turning it ...

Page 17: ... circular image then the interpupillary distance is set correctly 5 4 Dioptre adjustment The eye strengths of each eye of the microscope user can often be slightly different which in daily life has no consequences But when using a microscope this can cause problems in achieving precise focussing You can use a mechanism on the left tube connector dioptre adjustment ring to compensate for this as fo...

Page 18: ...ual objectives you should use the silver ring above the objectives see illustration When rotating the nosepiece you must always make sure that the objective which is about to be positioned in the beam path does not touch the object holder This can lead to significant damage to the objective lens We recommend that you always check from the side to make sure that there is sufficient leeway If this s...

Page 19: ...ion However this is regarded as Fixed Koehler since the condenser is pre centred When adjusting the Koehler lighting for the first time you must first select the lowest possible objective magnification so that you can carry out the following steps 1 Use the condenser focus wheel to position the condenser directly below the specimen stage Switch on the lighting and use the coarse and fine adjustmen...

Page 20: ...the illustration on the right The diameter of the aperture diaphragm which is then visible should make up approximately 2 3 of the pupil diameter If the eyepiece should be removed for checking then please make sure that no dirt or dust falls into the tube 6 It is possible to alter the brightness of the bulb using the dimmer The brightness is always controlled by the bulb brightness and not by the ...

Page 21: ...glasses symbol on the side They are also marked in the item description by an additional H example HSWF 10x Ø 23 mm When fitting the eye cups make sure that the dioptre setting is not moved We would therefore advise that you hold the dioptre compensation ring on an eyepiece with one hand while you fit the eye cup with the other Before using the microscope users who wear glasses must remove the eye...

Page 22: ...slide 2 Lower the specimen stage and position the 100x objective in the beam path 3 Bring the specimen stage or object slide to the objective very slowly until there is slight contact 4 Observe the object The object slide and objective must not be pressed against each other The oil constitutes the contact layer If the contact is made too jerky there is a chance that existing air bubbles in the oil...

Page 23: ...side of the device and the screws replaced the bulb replacement procedure is complete Important When fitting the new bulb into the socket it must only be handled with sterile gloves or using the bulb packaging film Grease and dust residue can have a negative effect on the light quality and service life LED Before changing the bulb the device must be switched off and unplugged To change the bulb ti...

Page 24: ... The polarisation unit consists of a polariser and an analyser Both consist of a round glass plate with an external holder ring To mount the analyser you have to remove the microscope head at first Then the analyser has to be inserted into the round aperture of the beam path right above the nose piece The polariser on the other hand is only placed on the ring holder of the field lens see right ill...

Page 25: ...ng the PC screen which is connected The tube has a light distribution that guarantees the providing of light for the eyepieces and the camera connection at the same time This means that it is possible to simultaneously observe by the eyepieces and PC screen For C mount adapters which have their own integrated magnification the image which is shown on the camera connected to the device can often ha...

Page 26: ...ic magnification 10x 20x 40x or 100x a PH slider which is adapted to the lens being used a centring telescope and two green filters To use this you need to replace the standard condenser of the microscope with the PH condenser One of the objectives in the nosepiece is also replaced with the PH objective and this is positioned in the beam path ...

Page 27: ...to one another in the beam path The position of the ring in the objective cannot be altered that of the ring in the slider however can be Although it has been pre centred it may still be necessary that after a certain period of use it will need to be re centred using the three Allen bolts on the side of the slider and the centring telescope see illustration When installing the centring telescope i...

Page 28: ...is contained in the spectrum of the light source HBO or LED The excitation filter is only permeable for the according wave length After that the excitation light hits a dichroic mirror which reflects it towards the objective and the sample After the sample absorbed the excitation light the emission of the fluorescent light occurs with a bigger wave length than the excitation light The part of the ...

Page 29: ...OBF 1_OBL 1 BA e 1510 28 Overview of wave lengths for excitation and emission per excitation filter Blue line Wave length excitation Green line Wave length emission ...

Page 30: ...29 OBF 1_OBL 1 BA e 1510 ...

Page 31: ...OBF 1_OBL 1 BA e 1510 30 ...

Page 32: ...Cover for FL module Fixing Allen screw for microscope head Main body Connection point for microscope head Fixing screw for lamp housing 7 8 9 10 11 HBO LED lamp housing Power connection Screw for attaching the protective plate Connection point for microscope housing Allen wrench ...

Page 33: ...ing device for field diaphragm Control lever for field diaphragm Centring device for aperture diaphragm Control lever for aperture diaphragm 6 7 8 9 10 Condenser control Screw for cover of lamp housing Centring screw for lamp vertical Centring screw for lamp horizontal Control lever for illumination ...

Page 34: ...fferent fluorescence reflected light units available for the microscopes of the series OBL 1 They have the following specifications Article number Illumination Excitation filter Centring objective Input voltage power supply unit OBB A1154 100W HBO B G Yes 100 240V OBB A1157 3W LED B G Yes 100 240V ...

Page 35: ...se the reflected light unit for special fluorescence applications it will be favourable to adjust the microscope in the bright field mode This includes the placing of the sample adjusting the interpupillary distance pre focussing dioptre adjustment etc After that you can go on with the usage of the fluorescence reflected light unit 1 When using a HBO lamp at first there must be ensured that the co...

Page 36: ...cant reduction of the lamps life time Equally the lamp must not be switched on directly after a switch off process When pausing the observation you should always slide in the control lever for the illumination in order to interrupt the light beam The light spectrum of the HBO lamp often can be harmful for microbes Never look inside the eyepieces when the beam path is opened by the control lever fo...

Page 37: ...pe 5 Use the two centring screws on the lamp housing for moving the light spot into the centre of the hair cross Warning Heat development on the lamp housing Changing the lamp HBO 1 Disconnect the FL reflected light unit from the power supply 2 Check if the lamp housing has cooled down 3 Loosen the screw for the cover of the lamp housing only when it is cool 4 Carefully flip out the lamp holder 5 ...

Page 38: ...to the mains power supply socket With the device switched off and unplugged you can screw out the housing The defective fuse can be removed from its housing and be replaced with a new one After that you just need to insert the fuse housing back into the insertion point next to the mains power supply socket ...

Page 39: ...ectly centred The condenser is too low The field of view is dark or is not correctly illuminated The objective is not positioned correctly on the beam path The selector switch for the beam path is between two settings The nosepiece is not correctly fitted The condenser is not correctly fitted An objective is being used which doesn t match the lighting area of the condenser The condenser has not be...

Page 40: ...ces Dirt dust on the front lens of the condenser Dirt dust on the object One side of the image is blurred The stage was not correctly fitted The objective is not positioned correctly on the beam path The nosepiece is not correctly fitted The upper side of the object is facing down The image flickers The nosepiece is not correctly fitted The objective is not positioned correctly on the beam path Th...

Page 41: ...ndly materials which you can dispose of at your local recycling centre Disposal of the storage box and device must be carried out by the operator in accordance with all national or regional laws in force in the location of use 12 Further information The illustrations may differ slightly from the product The descriptions and illustrations in this user manual are subject to change without notice Fur...

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