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EM Suite 15/11/2016 

 

 

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1.

 

‘BRIGHTNESS’ knob (left console) 
- CLOCKWISE (CW) – spread beam = decrease illumination  
- ANTI-CLOCKWISE (ACW) – condense beam = increase illumination 

2.

 

CRS button –any controls are in the coarse mode (16-fold change) when the nearby is 
illuminated 

3.

 

right console F1 button: ‘Raise fluorescent screen’  

4.

 

right console F5 button: ‘Beam Blank’ 

5.

 

Double press ‘LF2’ button (left console): exchange sample – X, Y and Z neutralize and 
filament is turned off. 

6.

 

In case of beam lost. Press ‘LF3’ button (left console). Reloads current saved alignment 
settings for the microscope. 

7.

 

‘Nanospace Map’ window: use it to move to specific areas on the stage and to memorize 
different positions (select point, then click ‘Shoot’)  

 

Red point = current position 

 

Yellow square = memories 

 

Green square = current selected position 

 

Press Memory - position is stored. Text can be entered in the Memo column 

 

To delete a position – select position from the list and press ‘Delete’ 

 

To move stage to position needed – left click on the map where you want to be and 
press ‘Shoot’. 

8.

 

Do not change MAGNIFICATION when WOBBLER is on – microscope can glitch and 
focusing become impossible. If that happened, close TEM Center and open it again 

9.

 

It is good practice to protect the chip of the camera from excess illumination using the 
‘Brightness MAG link’ facility (See TEM center). 

 

Select a magnification of 25,000x 

 

Set the illumination using the camera 

 

Switch on ‘Brightness MAG link’ in the Illumination System window – the 
illumination will now be maintained through the magnification range, up or down. 

 

 

Summary of Contents for JEM1400

Page 1: ...cuum monitor window Check that the gun and column vacuum is reasonable all chambers except Specimen chamber should say Evac Ready 5 JEOL computer Use beam controller window If the High tension HT is off turn it on It should be set to 80 03 kV if a different value is needed ask EM suite staff 6 Aperture settings Check it on the microscope column condenser aperture should be IN adjust with lever lef...

Page 2: ...ent positions select point then click Shoot Red point current position Yellow square memories Green square current selected position Press Memory position is stored Text can be entered in the Memo column To delete a position select position from the list and press Delete To move stage to position needed left click on the map where you want to be and press Shoot 8 Do not change MAGNIFICATION when W...

Page 3: ...ver to diffraction mode You should see a circle with bright spot in the centre If bright spot is not condensed use DIFF FOCUS button right console to condense the bright spot turn and see which direction the spot gets condensed Centre the spot if it is not centred Press PLA button left console and use DEF STIG knobs X and Y Adjust X and Y knobs on objective aperture until bright spot become in the...

Page 4: ...zed You will be at or near the focus point The image wobbler is useful for rough focusing at magnifications up to 50 000 Turn off the wobbler when focus is established and before recording an image 11 Record images see instructions below 12 LOW MAGNIFICATION imaging Switch from normal magnification mode Choose magnification of 800x using MAG CAML knob right console Press LOW MAG button right conso...

Page 5: ...older stops while still pushing the holder turn switch from Air to Pump you must pull switch out and push it up You should hear the valve opening wait for green light to come on after green light comes on get a good grip on the specimen holder and rotate clockwise a few 10o degrees until it stops and then allow it to go into the microscope a short distance 0 5cm until it stops don t let the holder...

Page 6: ...i clockwise several 80o degrees until it stops if you feel resistance STOP and get help something is wrong pull holder out a short 5mm distance until you can turn the holder further a few 10o degrees anti clockwise until it stops turn switch from Pump to Air you must pull switch out and push it down switch on Nitrogen gas open butterfly valve on the wall wait for amber and green lights to turn off...

Page 7: ...ss HT wobbler button right console the beam will start consistently condensing spreading Use binoculars and small fluorescent screen Adjust brightness BRIGHTNESS knob to comfortably see the feature The image should pulse on center If not press Bright Tilt button left console and use DEF STIG knobs X and Y on left and right consoles to align Turn off Bright Tilt and HT wobbler buttons Hint do one k...

Page 8: ...knobs X and Y to achieve a concentric halo around the image of the filament tip Cancel F4 Gun Align button Increase filament heating Use Target value field Beam controller window and go up 10 so only a slight shadow of filament is visible Use SHIFT knobs X and Y to move the beam to the centre 6 Adjust condenser lens astigmatism Set magnification 15 000x using MAG CAML knob right console Centre the...

Page 9: ...odes list New FFT window opens black background with white shape in the centre Use FOCUS coarse Go through focus forward and backward and try to maximise size of the white shape so its form become more visible The white shape should be circular if not need to be adjusted To adjust press OBJ STIG button left console Use the DEF STIG knobs X and Y to get white shape circular form Press BRIGHT TILT b...

Page 10: ... opened Safe image window click Save with caption You can add some extra information about the image If applicable Line1 Block number and BoxName_SlotNumber Line2 Region of interest ROI 12 Save your images on drive D folder Images Save in the following manner Initial folder is user name LastName_FirstName The date YEAR MONTH in numerical format XXXX XX should be included in a subfolder name Projec...

Page 11: ...e 11 14 15 DO NOT install any software including drivers on the AMT computer If your drive will not work without installing drivers on the AMT computer then find another drive that will 16 DO NOT plug anything into JEOL computer ...

Page 12: ...lf of the screen center beam if uncentered SHIFT knobs X and Y on the left and right consoles 3 Turn off filament Use beam controller window Press Filament OFF button 4 Record the time off in the log book 5 Remove specimen follow instructions above 6 Insert empty holder back into microscope follow instructions above 7 Close AMT camera program 8 Turn off monitors and lights ...

Page 13: ...h off Switch off the AMT computer Switch on the JEM 1400 as below Switch on the JEM 1400 Press the button EM will start Switch on the AMT computer and camera control Switch on the HP computer Log on using the HP keyboard and JEOL MOUSE at rear of EM Check for communications TEM Center loads automatically Check vacuum status to ensure the correct valves are open Ensure the lenses are on Wait RT1 Pi...

Page 14: ...controller window HT voltage ON may already be on i e green Filament ON Bias adjust Bias Coarse or Bias Fine to change the beam current Need 12 16µA emission For 80kV the beam current 44µA Therefore the total 56 59µA kV Beam current 40 22µA 60 33µA 80 44µA 100 55µA 120 67µA ...

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