EM Suite 15/11/2016
P a g e
1 | 14
JEM1400 Transmission Electron Microscope operation
Starting Session
1.
Turn on all computer monitors
2.
On the JEOL computer, if not logged in, select JEMUser account (password ‘JEMUser’)
and log in (you might need to use JEOL computer mouse and keyboard at the back of
JEOL monitor). After this, the computers should be controllable by AMT computer mouse
and keyboard. If not, notify EM Suite staff.
3.
JEOL computer. If ‘TEM center’ is not opened, double click ‘TEM center domain’ icon on
desktop.
4.
JEOL computer. Use ‘Valve/vacuum monitor’ window. Check that the gun and column
vacuum is reasonable - all chambers except ‘Specimen chamber’ should say ‘Evac Ready’.
5.
JEOL computer. Use ‘beam controller’ window. If the ‘High tension (HT)’ is off, turn it on.
It should be set to 80.03 kV (if a different value is needed ask EM suite staff).
6.
Aperture settings. Check it on the microscope column.
condenser aperture should be IN (adjust with lever; left is in, right is out).
objective aperture should be OUT (red dot is out, white dots – in and show relative
size of aperture). The objective aperture may be IN after you know you have a beam
and if you want the extra contrast.
7.
Spot size – 2. Use ‘TEM System Task Bar’ window. To change use ‘SPOT SIZE’ knob (left
console).
EM
AMT camera
computer
JEOL computer
