Manual for CD spectrometer
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Go through the “Switch ON procedure” only 1-2 hours before using it!
-
Turn on the N
2
flow on the “wall column”
. Only use the black wheel (not the pressure-reducing
valve).
- Turn on the “red box” (behind the box).
- Adjust the N
2
flow on the little black wheel to 12-20l/min.
-
Turn on the cooling water
N
2
flow is 12-20l/min and the cooling water is on? If yes go ahead:
- Tun on lamps of the power supply: POWER1, then POWER 2
- Switch on the temperature controller (PTC-348MI) and press “Start”.
- Start the computer and start the software on the desktop.
Sample Preparation:
1) Prepare your sample in a volumetric flask, don’t fill it into a cuvette.
Check on the UV/vis spectrometer, wheter you are in the right concentration range.
- Strong chromophores: c ≈ 10
-5
M. If necessary, start diluting it after a first quick measurement.
- Weak chromophores: c ≈ 10
-4
M. If necessary, start diluting it after a first quick measurement.
- GENERAL RULE: your absorbance band of interest should be at 0.1-0.8 E, ideally at around
0.2-0.4 E.
2) Fill your cuvette with pure solvent.
Start your measurement: (after the machine warmed up for 1-2 hours)
- Choose “Setup”, then setup some parameters which you find in the submenus.
- Insert your cuvette with pure solvent into the machine and record a Baseline. Save it.
- Remove cuvette, empty it, blow it out with N
2
and fill your sample solution. Put it into the
machine.
- Measure your sample.
- Measure as many samples as your want, all will be automatically be corrected your baseline
file.
Turn off the machine:
- close the Specta Manager software shut down the computer
- turn off the PTC-348WI turn off Power 2 switch
- turn off Power 1 switch
- purge instrument with N
2
for 5 minutes after shutdown before turning off the nitrogen flow.
