Invitrogen Qubit 4 User Manual Download

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For Research Use Only. Not for use in diagnostic procedures.

Qubit

™

4 Fluorometer

Catalog Number

Q33226

Publication Number

MAN0017209

Revision

A.0

Summary of Contents for Qubit 4

Page 1: ...For Research Use Only Not for use in diagnostic procedures Qubit 4 Fluorometer Catalog Number Q33226 Publication Number MAN0017209 Revision A 0...

Page 2: ...ription A 0 05 October 2017 New user guide Important Licensing Information This product may be covered by one or more Limited Use Label Licenses By use of this product you accept the terms and conditi...

Page 3: ...al Use Reagent Calculator to prepare Qubit Working Solution 18 Calibrate assays on the Qubit 4 Fluorometer 20 Read samples 24 Results 26 Fluorometer mode 29 Ion Sphere Assay 31 4 Manage data 32 Introd...

Page 4: ...59 Qubit 4 Fluorometer and accessories 59 Appendix C Safety 60 Symbols on instruments 60 Safety labels on instruments 61 General instrument safety 62 Chemical safety 63 Chemical waste safety 64 Elect...

Page 5: ...listed below For safety alert words and symbols used in Thermo Fisher Scientific user documentation see page 4 Convention Use Bold Bold text indicates user action For example Click Run Right arrow sy...

Page 6: ...tuation that if not avoided could result in death or serious injury DANGER Indicates an imminently hazardous situation that if not avoided will result in death or serious injury This signal word is to...

Page 7: ...the description and specifications of the Qubit 4 Fluorometer Upon receiving the instrument Examine the instrument carefully for damage incurred during transit Ensure that all parts of the instrument...

Page 8: ...ple High levels of accuracy using only 1 20 L of sample even with very dilute samples Use of dyes selective for dsDNA RNA or protein minimizes the effects of contaminants in the sample Stores results...

Page 9: ...B allows you to transfer your data directly to your computer using the USB cable supplied with the instrument or any other similar USB cable USB drive ports Type A allow you to transfer and save data...

Page 10: ...door use only Operating temperature 10 40 C Operating humidity 20 80 non condensing Technical specifications Dynamic range 5 orders of magnitude Processing time 5 seconds sample Light sources Blue LED...

Page 11: ...powers on first displaying the splash screen and then the Home screen 6 To power down the Qubit 4 Fluorometer unplug it IMPORTANT Use the power cord plug adapter supplied with the instrument that is a...

Page 12: ...n about your Qubit 4 Fluorometer including the currently installed software version To access the About Instrument screen 1 On the Home screen press Settings 2 On the Settings screen press About Instr...

Page 13: ...ng instrument settings for the Qubit 4 Fluorometer Sleep mode page 12 Brightness page 12 Date Time page 13 Reset instrument page 14 Language page 15 Access the Instrument settings screen 1 On the Home...

Page 14: ...field then using the number pad to select a value The software requires a minimum of 1 minute and a maximum of 60 minutes 3 Press Done to save the changes and return to the Instrument settings screen...

Page 15: ...te format select a time format then press Next 3 Enter the date and time a Press a date field day month or year then use the number pad to select a value b Press a time field hours or minutes then use...

Page 16: ...t function is not reversible To return the instrument to its default factory settings 1 On the Instrument settings screen page 11 press Reset instrument to display the Reset Qubit 4 screen 2 Press Res...

Page 17: ...German Italian Spanish simplified Chinese and Japanese 1 On the Instrument settings screen page 11 press Language to display the Language screen 2 Select a language then press Next 3 Press Yes to con...

Page 18: ...the Qubit DNA and RNA assays for 2 minutes after mixing the sample or standard with the working solution Incubate the tubes for the Qubit protein assays for 15 minutes after mixing the sample or stand...

Page 19: ...our samples see page 59 for available Qubit assay kits and ordering information DNA RNA or protein samples Qubit assay tubes or other appropriate 0 5 mL assay tubes Appropriate standards for the assay...

Page 20: ...ffer required to make the appropriate amount of Qubit Working Solution required to prepare your samples and standards Use the Reagent Calculator 1 On the Home screen press Reagent Calculator 2 Enter t...

Page 21: ...ount of Qubit dye and buffer required to prepare the Qubit Working Solution with these inputs Note You can change the total number of tubes that you plan to run or the overage selection on this screen...

Page 22: ...nstructions for running new calibration standards and for using the last calibration Read new standards for calibration 1 On the Home screen select the assay type for which you wish to read new standa...

Page 23: ...rompt insert Standard 1 into the sample chamber and press Read standard Be sure to use the Standard 1 appropriate for the assay you are performing The reading takes approximately 3 seconds 5 At the pr...

Page 24: ...lues for Standard 1 and Standard 2 and Standard 3 if applicable These values can assist you in making a judgment regarding the calibration results For the Qubit dsDNA BR Qubit dsDNA HS Qubit ssDNA Qub...

Page 25: ...eview the Read standard screen see Note on page 22 3 If you wish to re run the standards or run new standards press Read standards then repeat the calibration procedure beginning on page 18 Note For t...

Page 26: ...for the Qubit protein assay Read samples 1 In the Read standard for Qubit quantitation assays or Ready for samples screen for Qubit RNA IQ assay press Run samples 2 In the Sample volume screen select...

Page 27: ...conds for the quality assays 4 The software displays the results See Results screen on page 26 Results screen for quantitation assays Results screen for quality assays Read multiple samples To read mu...

Page 28: ...tration values are displayed The top value in large font is the concentration of the original sample The bottom value is the dilution concentration the concentration of the sample in the tube inserted...

Page 29: ...samples that fall within the assay s core range Yellow circles represent samples that fall within the assay s extended range Red circles represent samples or standards that fall outside the assay s r...

Page 30: ...e RNA IQ is based on a scale of 1 10 similar to other RNA quality scores A small RNA IQ in the assay indicates that the sample is comprised of mainly small RNA and a larger RNA IQ indicates that the s...

Page 31: ...to choose the excitation light source blue LED or red LED while reading fluorescence in both the green and far red emission channels for the blue LED or in the far red emission channel only for the re...

Page 32: ...mission and the far red emission of your sample For Red excitation the Qubit 4 Fluorometer displays only the far red emission of your sample Blue 470 nm excitation Red 635 nm excitation Read multiple...

Page 33: ...d a complementary red fluorescent Alexa Fluor 647 dye labeled oligonucleotide The Qubit 4 Fluorometer measures the fluorescence emission of each dye conjugate and a Microsoft Excel file template is us...

Page 34: ...allows you to View detailed data for each sample page 32 Rename data files page 35 Save data as a CSV comma separated value file and export the CSV file to a USB drive or directly to your computer pa...

Page 35: ...n displaying a list of data entries for that data set appears To view additional data entries that do not fit in the screen scroll up or down Data screen for quantitation assays Data screen for qualit...

Page 36: ...34 Qubit 4 Fluorometer User Guide 4 To view the sample details press the sample of interest A Data details screen opens Data details for quantitation assays Data details for quality assays...

Page 37: ...n select the data set of interest 3 From the list of data entries for the selected data set press the sample of interest to open the Data details screen Data screen for quantitation assays Data screen...

Page 38: ...tly to the computer using the USB cable You can then import the file into any spreadsheet program Export data using the USB drive 1 Insert the USB drive into the Qubit 4 Fluorometer 2 On the Concentra...

Page 39: ...one provided with the Qubit instrument The USB cable must remain connected to both the Qubit instrument and the computer during this process To export data directly to a computer 1 Connect the Qubit...

Page 40: ...User Guide Information saved to the CSV file Note For the RNA IQ assay there is no original sample concentration Qubit tube concentration or dilution factor Instead the results file contains large and...

Page 41: ...left of each data set you wish to delete To delete only individual data entries from a data set press the data set of interest and then check the selection box to the left of the samples you wish to...

Page 42: ...from our website at thermofisher com qubit or create new assays using the MyQubit software and web tool Manage assays Manage assays function The Manage assays function allows you to hide and show unhi...

Page 43: ...ish to show or hide The software displays a list of existing individual assays within the selected assay folder and the controls for showing and hiding them 4 To hide all of the assays in the assay fo...

Page 44: ...lays a list of existing individual assays within the selected assay folder and the controls for showing and hiding them 4 To hide an individual assay swipe the Assay On Off switch located to the right...

Page 45: ...rs and individual assays and activates the move function 3 Press and hold on the desired assay folder or individual assay to detach it from its current position then drag it to its new location To mov...

Page 46: ...e screen 6 Press the Home button to return to the Home screen In the Home screen you can press and drag the selected assay to another assay folder or location as described in Move assay folders indivi...

Page 47: ...rmofisher com qubit and download the MyQubit assay files qbt file you wish to install to the root directory of a USB drive Do not download the qbt files to a folder in the USB drive 2 Insert the USB d...

Page 48: ...turn to the Settings screen without importing the assays Note Each assay folder can contain up to 9 individual assays The Home screen can contain up to 36 assays or folders 6 Select the destination fo...

Page 49: ...e Import new assay Where screen The newly created folder will appear on the list of folders Select the new destination folder then press Save The Verification screen opens see Step 9 page 48 8 In the...

Page 50: ...import queue then press Save Repeat this process until you have imported all the assays you have selected in Step 4 page 45 Once all the assays you have selected are imported successfully the Verifica...

Page 51: ...hould be recognized as an external device by the computer 3 Copy and paste or click drag the qbt files you wish to import to the Qubit instrument window on your computer Note The assays will not be vi...

Page 52: ...anging the existing assays Fluorometer mode allows you to use the Qubit 4 Fluorometer with any reagent or assay compatible with the optical settings of the instrument In this way the device can be use...

Page 53: ...ating the instrument IMPORTANT Using a cleaning or decontaminating method other than that specified by the manufacturer may result in damage to the instrument Clean the surface of the Qubit 4 Fluorome...

Page 54: ...e transfer and update described in the next steps are complete a On your computer find the Qubit software update file that you wish to upload b Copy and paste or click drag the software file to the Qu...

Page 55: ...Fluorometer User Guide 53 5 When the appropriate files are detected the Software update screen is displayed Press Update to update the software 6 When prompted press Restart to complete the software...

Page 56: ...s 1 On the Home screen press Settings 2 On the Settings screen press Device verification test Verification test screen opens 3 On the Verification test screen press Start test 4 When the test is compl...

Page 57: ...takes to read the fluorescence See Assay temperature page 58 Be careful not to spill sample into the sample chamber Promptly wipe any spills The Qubit assays are very sensitive and even small amounts...

Page 58: ...that you have prepared the Qubit working solution correctly 1 200 dilution using the buffer provided in the kit Ensure that you have prepared the standard tubes correctly 10 L of each standard in 190...

Page 59: ...his incubation period the fluorescence signal is stable for 3 hours at room temperature for all nucleic acid assays except the Qubit ssDNA assay which is stable for up to 30 minutes The Qubit protein...

Page 60: ...BR assay Qubit dsDNA HS Qubit ssDNA Qubit RNA HS Qubit RNA XR Qubit RNA IQ and Qubit protein assays show similar sensitivities over the same range Qubit 4 Fluorometer calibration For each assay you ha...

Page 61: ...46018 Qubit Assay Tubes 500 tubes Q32856 Qubit RNA IQ Assay Kit 75 assays 275 assays Q33221 Q33222 Qubit RNA XR Assay Kit 20 1 000 ng 100 assays 500 assays Q33223 Q33224 Qubit RNA HS Assay Kit 5 100 n...

Page 62: ...uments Symbol Description Indicates that you should consult the manual for further information and to proceed with appropriate caution Indicates the presence of an electrical shock hazard and to proce...

Page 63: ...ns for handling and disposal ATTENTION D chets dangereux Lire les fiches techniques de s ret de mat riels et la r gulation locale associ es la manipulation et l limination des d chets DANGER High volt...

Page 64: ...tion Do not install the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Plug the power cord firmly into the wall outle...

Page 65: ...nimize the hazards of chemicals Read and understand the Safety Data Sheets SDSs provided by the chemical manufacturer before you store handle or work with any chemicals or hazardous materials See Safe...

Page 66: ...thing For additional safety guidelines consult the SDS Minimize the inhalation of chemicals Do not leave chemical containers open Use only with adequate ventilation for example fume hood For additiona...

Page 67: ...wer DANGER ELECTRICAL HAZARD Grounding circuit continuity is vital for the safe operation of equipment Never operate equipment with the grounding conductor disconnected DANGER ELECTRICAL HAZARD Use pr...

Page 68: ...des lunettes de protection appropri des v tements et des gants In the U S U S Department of Health and Human Services guidelines published in Biosafety in Microbiological and Biomedical Laboratories...

Page 69: ...icular installation If this equipment does cause harmful interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to corr...

Page 70: ...ian EMC standards This instrument has been tested to and complies with standard AS NZS CISPR 11 Limits and Methods Measurement of Electromagnetic Disturbance Characteristics of Industrial Scientific a...

Page 71: ...rmation about customer training Download software updates and patches Safety Data Sheets SDS Safety Data Sheets SDSs are available at thermofisher com support IMPORTANT For the SDSs of chemicals not d...

Page 72: ...thermofisher com support thermofisher com askaquestion thermofisher com 05 October 2017...

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