18
Calibrating the Qubit
®
2.0 Fluorometer,
Continued
Checking
Standards
The Check Standards Screen displays the raw fluorescence values
for Standard #1, Standard #2, Standard #3 (if applicable), and the
last measurement taken for a given assay. These values can assist
you in making a judgment regarding the performance of the
Qubit
®
2.0 Fluorometer and whether or not the samples you used
were within range.
For the Qubit
™
dsDNA BR, Qubit
™
dsDNA HS, Qubit
™
ssDNA, Qubit
™
RNA, and Qubit
™
RNA BR assays, the reading
given by Standard #2 should be much higher than that of
Standard #1, and the reading of your sample should fall
somewhere between that of the two Standards.
For the Qubit
™
protein assay, the reading given by Standard
#3 should be much higher than that of Standard #2, and the
reading of your sample should be no more than 50% greater
than Standard #3.
To access the Check Standards Screen, press
Check Stds.
on
the Standards Screen. The raw fluorescence values for the
standards and the last sample will be displayed on the screen.
