Invitrogen Bandmate MAN0018722, User Manual

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Calibration
The pumps that deliver wash buffer to the tray have been calibrated at the factory. To check or
re-calibrate the pumps, use the
Calibration function. Pumps should be re-calibrated every 6 months or
when moving the instrument from room temperature to a cold room or vice versa. The temperature of
the environment can impact the dispensed volume.
1.
Prior to calibration of the pumps, confirm that the wash buffer line is completely full of liquid. If
there are bubbles in the line, use the
Flush function to remove them.
2.
Open the waste compartment and remove the funnel trays and sample trays. If using a larger
graduated cylinder and extra room is needed, remove the waste tank. Alternatively, detach the
hose from the hose nozzle and place it in the graduated cylinder.
Note: Be careful not to stretch or distort the hose.
3.
Place the graduated cylinder under the buffer, position as indicated on the graphical display, and
press
Fill to dispense liquid into the cylinder. The pump will dispense approximately 40 mL.
4.
Once the pump has stopped running, measure the actual amount of liquid dispensed and enter the
value using the encoder knob. Measure the volume from the other positions in the same way and
record the value. Press
Save . The pumps are now calibrated.
5.
Replace the waste tank and trays, then close the waste compartment.
The amount of liquid dispensed by the pumps is temperature-dependent. This is accounted for in
the factory calibration.
The Bandmate
™
processor calibrates based on temperature. "Warm" calibration is indicated by the
red numbers, while "cold" calibration is indicated by the blue numbers. These numbers represent the
pump coefficients, ranging from 100 to 300 with 200 being the intrinsic value of the pump. The cutoff
temperature between "warm" and "cold" is 12°C. To check the calibration and use the processor
at different temperatures, perform a "warm" and a "cold" calibration at the warmest and coldest
temperatures at which the processor is planned to be used.
If the processor is only used at one
temperature, it is unnecessary to calibrate it at any other temperature.
Chapter 1
Bandmate
™
Automated Western Processor
Operate the Bandmate ™ Automated Western Blot Processor
1
Bandmate ™ Automated Western Blot Processor User Guide
11