Invitrogen Bandmate MAN0018722 User Manual Download

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For Research Use Only. Not for use in diagnostic procedures.

Bandmate

™

Automated Western Blot

Processor

USER GUIDE

For automation of western blot processing

Catalog Numbers

BW1000

Publication Number

MAN0018722

Revision

C.0

Summary of Contents for Bandmate MAN0018722

Page 1: ...ch Use Only Not for use in diagnostic procedures Bandmate Automated Western Blot Processor USER GUIDE For automation of western blot processing Catalog Numbers BW1000 Publication Number MAN0018722 Rev...

Page 2: ...sion history Pub No MAN0018722 Revision Date Description C 0 14 Sep 2021 Added to products for separate purchase and troubleshooting topics Minor corrections B 0 06 Oct 2020 Corrected safety and regul...

Page 3: ...connection symbols 16 Conformity symbols 16 Safety information for instruments not manufactured by Thermo Fisher Scientific 17 Instrument safety 17 General 17 Physical injury 18 Electrical safety 18 C...

Page 4: ...mes Antibodies and reagents are directly streamed into incubation trays which eliminates cross contamination between reagents The curved mini and midi trays allow for even processing of blots with min...

Page 5: ...or Siphon Hose for Buffer Bottle 1 Reagent Tubes 40 50 mL Recovery Tubes 8 Mini Trays 2 Midi Trays 2 Tray Covers 2 Funnel Trays 2 Waste Trays 2 Quick Reference Guide 1 Table 3 Available for separate p...

Page 6: ...le Protein Stain Kit for PVDF Membranes 24585 Operate the Bandmate Automated Western Blot Processor Set up the system 1 Set up the processor on a stable level lab bench 2 Open the door and remove the...

Page 7: ...and as a template Select the desired program using the dial Press Edit 2 Enter a name for the custom protocol Select letters and numbers by turning the dial Use the cluster of 4 buttons at the lower l...

Page 8: ...if antibodies need to be saved Table 6 Standard Midi Step Reagent Repeat Fill volume Rock time Rocks per min Drain to 1 1 Pre Fill 1X 1 hr 15 Waste 2 1 1X 1 hr 15 Waste 3 Buffer 3X 40 mL 10 mins 20 W...

Page 9: ...two reagent tubes will be required for each blot being processed Check to ensure the foil on the bottom of the reagent tubes is not damaged Screw one reagent tube firmly and completely into the prima...

Page 10: ...tinue to rock with the final dispensed wash buffer volume in the tray after all of the steps of the protocol are completed to ensure that the blots do not dry out The Home button should be selected be...

Page 11: ...the pump has stopped running measure the actual amount of liquid dispensed and enter the value using the encoder knob Measure the volume from the other positions in the same way and record the value P...

Page 12: ...um washes per step 10 Minimum reagent per blot 3 5 mL mini 7 5 mL midi Maximum number of protocol steps 9 Maximum number of protocols 7 Buffer dispensing volume 10 150 mL Incubation step time range 0...

Page 13: ...the sample tray remain at a 90 angle to the horizontal are not bent over and that they are still sharp If the pokers are bent it may be possible to bend the pokers back to their original vertical pos...

Page 14: ...will appear The reagent tubes were not pierced Possible issues can include a faulty tube bent piercing points on the incubation tray or faulty alignment of the reagent slider Verify that all tubes hav...

Page 15: ...see the Documentation and Support section in this document Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information In...

Page 16: ...s conformity with European Union requirements Indicates conformity with Australian standards for electromagnetic compatibility Indicates conformity with the WEEE Directive 2012 19 EU CAUTION To minimi...

Page 17: ...and Pressurized fluids Wear eye protection when working with any pressurized fluids Use caution when working with any polymeric tubing that is under pressure Extinguish any nearby flames if you use fl...

Page 18: ...and hold it stationary while someone else slides the contents out of the box Electrical safety WARNING Do not overfill trays with liquid Excess liquid can overflow into the control unit and possibly...

Page 19: ...re recommended by the manufacturer confirm with the manufacturer that the proposed method will not damage the equipment Instrument component and accessory disposal To minimize negative environmental i...

Page 20: ...igital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses a...

Page 21: ...trical and electronic equipment SJ T 11364 2014 China RoHS Standard Marking for the Restricted Use of Hazardous Substances in Electronic and Electrical Products For instrument specific certificates vi...

Page 22: ...primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet federal...

Page 23: ...goggles Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state...

Page 24: ...tocols Certificates of Analysis Safety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Tec...

Page 25: ......

Page 26: ...ated Western Processor_UG_MAN0018722 v6 GUID 8DBD70A8 7BC4 4AB0 919A 5601AB0A9781 2021 07 19 19 26 41 en 15 09 51 386 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 14 S...

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