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Multitron Pro - Incubation Shaker

Operation

2013-02-19

Page

103

of

185

7 Operation

7.1 Safety

Personnel

The incubator shaker may only be operated by qualified
chemistry, biology or bio-technology technicians or by
personnel who have been appropriately instructed by qualified

chemistry, biology or bio-technology technicians

.

All work associated with preparations for cultivation and the
cultivation process must be carried out by qualified chemistry,
biology or bio-technology technicians or by personnel who have
been appropriately instructed by qualified chemistry, biology or
bio-technology technicians.

Inappropriate operation

WARNING!
Risk of injury due to inappropriate operation!

Inappropriate operation may lead to severe
physical injury or loss of property.

Therefore:

– Strictly follow the instructions stated in this

operating manual.

CAUTION!
Risk of burns on touching hot surfaces.

There is a risk of burns from hot surfaces if the
table and/or cultivation flasks are touched when the
unit is operating at temperatures above 50 °C.

Therefore:

– Always wear safety gloves.

CAUTION!
Risk of injury due to broken glass or shards
from glass cultivation flasks.

Broken or splintered cultivation flasks can injure
hands and eyes.

Therefore:

– Always wear safety glasses.

– Always wear suitable safety gloves.

– Always use undamaged cultivation flasks.

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Summary of Contents for Multitron Pro

Page 1: ...Operating manual 2013 02 19 Multitron Pro Incubation Shaker Dok ID V 01 002 Original ...

Page 2: ...infors ht com Infors Benelux BV Fabriekstraat 38 7005 AR Doetinchem P O Box 613 NL 7000 AP Doetinchem T 31 0 314 36 44 50 F 31 0 314 37 80 76 infors bnl infors ht com Contact details of our local dealers worldwide can be found on our website www infors ht com Infors Canada 8350 rue Bombardier Anjou Quebec Canada H1J 1A6 T 1 514 352 5095 F 1 514 352 5610 infors ca infors ht com Infors Bio Technolog...

Page 3: ...onal protective equipment 15 2 5 Particular hazards 15 2 6 Step to take in the event of hazardous situations and at accidents 18 2 7 Environmental protection 18 2 8 Adhesive labels signs symbols 19 3 Technical data 20 3 1 Dimensions 20 3 2 Weights 23 3 3 Connection values 26 3 4 Specifications 33 3 5 Operating conditions 46 3 6 Emissions 47 3 7 Utilities 47 3 8 Identification plate 48 3 9 Interfac...

Page 4: ... 80 5 3 Packaging 81 5 4 Transport 81 5 5 Storage of the incubator shaker 82 6 Installation and initial operation 83 6 1 Safety 83 6 2 Location requirements for installation 84 6 2 1 Place of installation 84 6 2 2 Minimum distances 85 6 3 Removing the transport lock 86 6 4 Mounting the drain nozzle 87 6 5 Switching on the unit 88 6 6 Installing the incubator shaker 89 6 7 Setting the capillary the...

Page 5: ...the tray 123 7 12 Handling the flasks without pulling out the tray 127 7 13 Loading the trays 127 7 14 Using the Sticky Stuff adhesive matting 129 7 15 Interrupting operation 130 7 16 Operating the UV sterilisation 131 7 17 Operating the Direct Steam Humidification 131 7 18 Operating the cooling system 132 7 19 Starting illumination 133 7 20 Starting algae illumination 135 7 21 Operating the CO2 g...

Page 6: ... 12 Cleaning the Sticky Stuff adhesive matting 153 8 13 CO2 curtain 154 8 14 Finishing maintenance work 154 9 Interferences 155 9 1 Safety 157 9 2 Tables of interferences 158 9 3 Error messages 158 9 4 Interference Illumination broken tube 165 9 5 Returning for repair 166 10 Disassembly 167 10 1 Safety 167 10 2 Disassembly 168 10 3 Disposal 169 11 Examples 170 11 1 Delay a speed change 170 11 2 Sw...

Page 7: ...rstand this manual before commencement of any work Adhering to all the points advice and instructions concerning safety and operation in this operating manual is a pre condition for safe working Furthermore local rules for accident prevention and general safety regulations relevant to the equipment s field of application may be in force This manual contains pictures which aid general understanding...

Page 8: ...perty DANGER points out an immediate dangerous situation that leads to death or severe injuries unless avoided WARNING points out a potentially dangerous situation that may lead to death or severe injuries unless avoided CAUTION points out a possibly dangerous situation that leads to slight or minor injuries unless avoided CAUTION points out a possibly dangerous situation that leads to damage to p...

Page 9: ... standards and regulations as well as the current state of technology the manufacturer s knowledge and experience The manufacturer will not be held responsible for losses arising from Non observance of the points listed in the operating manual Incorrect and inappropriate use of the equipment Unqualified personnel using the equipment Arbitrary modifications Unauthorised technical changes Arbitrary ...

Page 10: ...and or may lead to damage malfunction or complete breakdown Therefore Use only original spare parts from the manufacturer Spare parts may be purchased from an authorised dealer or direct from the manufacturer See address on page 2 1 6 Terms of Guarantee The terms of the guarantee are included in the manufacturer s general conditions of business contract to supply 1 7 Customer Service Our customer ...

Page 11: ...ssment to identify additional hazards due to special working conditions related to the equipment s area of application They must declare these hazards in the form of directives for the equipment s operation The operator must ensure that these directives comply with current legal regulations and adapt them as necessary The operator must clearly regulate and define responsibility for installation op...

Page 12: ...rds and regulations Qualified personnel are capable of carrying out the assigned work identifying and avoiding possible hazards independently due to their professional standing experience skills and knowledge of relevant standards and regulations Qualified personnel in biology biotechnology or chemical engineering are capable of carrying out work in the field of biology biotechnology or chemical e...

Page 13: ...or conventional uses as described here Conventional use of the equipment also includes following all instructions in this operating manual Each instance of non conventional use is considered as misuse and may lead to dangerous situations Danger by misuse WARNING Danger by misuse Misuse of the equipment may lead to dangerous situations In particular desist from using the equipment in any of the fol...

Page 14: ... larger working volumes as permitted are used Therefore Use only flasks with a plane bottom Working volume must not be more than 1 3 of the total volume of the flask Do not use flow diffusing inserts in the flasks Erlenmeyer flasks up to 5000 ml Fernbach flasks up to 3000 ml Other receptacles Test tube racks Microtiter plates Deep well plates The equipment is conventionally used only as a shaker f...

Page 15: ...thes with tight sleeves and no loose material Protective cap To protect against contamination and carryover of viable organisms Protective gloves chemical resistant To protect hands from aggressive substances Check gloves are impermeable before use Clean gloves before taking them off and store in a well ventilated location after use Safety goggles To protect the eyes against liquid splashes Safety...

Page 16: ...nd lock off any isolation switch before commencing maintenance cleaning or repairing Do not bypass fuses or take them out of operation Adhere to the correct rates in Amps when replacing fuses Keep components which are electrically powered away from humidity as excessive moisture may lead to short circuit Do not expose equipment to inappropriate environmental temperatures outside the stated operati...

Page 17: ...manual regarding gas supply and handling of gases and exhaust gas Moving parts WARNING Risk of injury due to moving parts Parts that are rotating or moving linearly can cause serious injuries Therefore Do not reach into moving parts nor work on or near moving parts Do not open covers while the equipment is operating Pay attention to the stopping time Make sure that all parts have stopped moving be...

Page 18: ... if additional isolation switches for the power supply have been installed locally Initiate first aid measures Remove people away from the danger zones care with handling Inform responsible personnel on site Alert emergency medical services if appropriate Clear access routes for ambulance 2 7 Environmental protection CAUTION Danger for the environment caused by mishandling Mishandling environmenta...

Page 19: ...y warning and operating instructions in a clearly legible state Replace damaged labels and stickers immediately Biohazards Warns against infectious biochemical substances e g liquids which contain bacteria or viruses present in the working area Some procedures must be executed on objects or in rooms which contain bacteria yeasts or other parasites These substances may become perilous not only to m...

Page 20: ...ncubation Shaker Technical data Page 20 of 185 2013 02 19 3 Technical data 3 1 Dimensions Drawing with side cooling Fig 1 Dimensions in Millimeters Drawing with ShakerBag gassing station Fig 2 Dimensions in milimeters ...

Page 21: ...ion Value Unit Width 1070 mm Single unit height 520 mm 2 unit staple height 1040 mm 3 unit staple height 1560 mm Base unit with door handle depth 880 mm Base unit with open door depth 1160 mm Base depth 715 mm Incubation chamber Description Value Unit Width 925 mm Depth 550 mm Hight 387 mm Hight with illumination inside 337 mm Volumn 200 L ...

Page 22: ... mm Cooling units Description Value Unit Side cooling width 245 mm Top cooling width 1070 mm Top cooling heigth 290 mm Direct steam humidification Description Value Unit Direct steam humidification depth 80 mm CO2 gassing Description Value Unit Width 30 mm CO2 pressure reducing unit Description Value Unit Width 40 mm ShakerBag gassing Description Value Unit Width 115 mm ...

Page 23: ...scription Value Unit Single unit 25mm throw 95 kg Additional weight 50 mm throw 13 kg 1 units stacked 25mm throw 98 kg 2 units stacked 25mm throw 202 kg 3 units stacked 25mm throw 305 Kg Approved load 25 throw incl tray Description Value Unit Standard unit with 25 mm throw up to 350 rpm over 350 rpm 9 to 19 12 to 16 kg kg Approved load 50 throw incl tray Description Value Unit up to 250 rpm over 2...

Page 24: ... mL 0 02 kg Clamp 100 mL 0 03 kg Clamp 250 mL 0 06 kg Clamp 500 mL 0 10 kg Clamp 1000 mL 0 15 kg Clamp 2000 mL 0 26 kg Clamp 2800 mL F3 0 30 kg Clamp 3000 mL 0 30 kg Clamp 4000 mL 0 35 Kg Clamp 5000 mL 0 45 Kg Perforated tray Description Value Unit Perforated tray 3 5 kg Base frame Description Value Unit Rubber feet 6 kg Trolley 10 kg Base frame 130 mm 26 kg Base frame 310 mm 27 kg ...

Page 25: ...am humidification Description Value Unit Direct steam humidification 4 5 kg Gassing ShakerBag Option Description Value Unit Gassing ShakerBag 3 kg Tray ShakerBag Option Description Value Unit ShakerBag Tray 12 kg Gassing unit CO2 Description Value Unit Gassing unit CO2 1 kg Illumination Description Value Unit Illumination unit Daylight Photosynthesis 12 kg Illumination algae Daylight Photosynthesi...

Page 26: ... 230 V Power consumption max 880 W Power consumption stand by 6 W Current max 3 8 A Individual fusing 16 A Frequency 50 60 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 115V 60 Hz Description Value Unit Voltage 115 V Power consumption max 880 W Power consumption stand by 6 W Current max 7 7 A Individual fusing 16 A Frequency 60 Hz 2 unit fuses 5 x 20 mm time lag 16 A ...

Page 27: ...al fuses 16 A Frequency 50 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 230 V 60 Hz Description Value Unit Voltage 230 V Power consumption max 690 W Current max 4 6 A Individual fuses 16 A Frequency 60 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 115 V 60 Hz Description Value Unit Voltage 115 V Power consumption max 570 W Current max 7 5 A Individual fuses 16 A Frequency 60 Hz 2 unit fuses 5 x 20 ...

Page 28: ...al fuses 16 A Frequency 50 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 230 V 60 Hz Description Value Unit Voltage 230 V Power consumption max 800 W Current max 3 5 A Individual fuses 16 A Frequency 60 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 115 V 60 Hz Description Value Unit Voltage 115 V Power consumption max 800 W Current max 6 9 A Individual fuses 16 A Frequency 60 Hz 2 unit fuses 5 x 20 ...

Page 29: ...ax 1 2 A Fuses None Frequency 50 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 230 V 60 Hz Description Value Unit Voltage 230 V Power consumption max 220 W Current max 1 2 A Fuses None Frequency 60 Hz 2 unit fuses 5 x 20 mm time lag 10 A Type 115 V 60 Hz Description Value Unit Voltage 115 V Power consumption max 200 W Current max 2 6 A Fuses None Frequency 60 Hz 2 unit fuses 5 x 20 mm time lag 16 A...

Page 30: ... 50 60 Hz Description Value Unit Power consumption max 125 W Current 0 4 A Type 115V 60 Hz Description Value Unit Power consumption max 125 W Current 0 9 A Electrical Heated door Description Value Unit Voltage 24 V Power consumption 18 8 W Current 0 8 A Electrical CO2 Gassing Description Value Unit Power consumption 4 5 W ...

Page 31: ...Power 25 W Power consumption max 0 1 A Type 115V 50 60 Hz Description Value Unit Electrical Power 25 W Power consumption max 0 2 A Electrical Illumination daylight photosynthesis Type 230V 50 60 Hz Description Value Unit Voltage 230 V Power consumption 180 W Current 0 8 A Type 115V 60 Hz Description Value Unit Voltage 115 V Power consumption 180 W Current 1 6 A ...

Page 32: ...s Type 230V 50 60 Hz Description Value Unit Voltage 230 V Power consumption 250 W Current 1 1 A Type 115V 60 Hz Description Value Unit Voltage 115 V Power consumption 250 W Current 2 2 A Electrical Mobile Pt100 sensor Description Value Unit Voltage 24 V Electrical UV sterilisation Description Value Unit Power consumption 11 W ...

Page 33: ...x 20 mm 10 Amperes time lag 115 V 60 Hz 2 x 20 mm 16 Amperes time lag Hose nozzle Ø inner diameter nozzle 8 mm Ø inner diameter hose 10 mm Temperature Description Value Power 2 cross flow blowers with heating 700 W Air circulation 360 m3 h Control Electronic PID controller Sensor Pt100 Operating range 4 C up to 80 C typically up to 65 C Control accuracy 0 2 C Measuring accuracy 0 15 C Control accu...

Page 34: ...nge No cooling 6 C over ambient temperature up to 65 C With cooling Side cooling 20 C under ambient temperature up to 65 C Top cooling 12 C under ambient temperature up to 65 C External cooling Depending on temperature of cooling liquid and ambient temperature Direct steam humidification Up to 40 C CO2 gassing Up to 60 C ShakerBag Option gassing Up to 40 C ...

Page 35: ...bital Control accuracy 1 at maximum speed Rotation direction Clockwise Range 20 400 rpm Shaking Variable throw Description Value Speed 20 350 rpm Control accuracy 4 rpm Speed 351 400 rpm Control accuracy 2 Throw 25 mm speeds Description Value Single unit 400 rpm Base 13 cm 2 units stacked bottom up to 400 rpm top up to 350 rpm 3 units stacked bottom up to 400 rpm middle up to 350 rpm top up to 350...

Page 36: ... rpm 2 units stacked bottom up to 350 rpm top up to 250 rpm 3 units stacked bottom up to 350 rpm middle up to 250 rpm top up to 250 rpm Base 31 cm 2 units stacked bottom up to 350 rpm top up to 250 rpm NOTICE For higher speeds contact the supplier Side cooling 380 W Power supply Voltage Frequency Power Consumption Rating 230 V 50 Hz 1 2 A 220 W 230 V 60 Hz 1 2 A 220 W 115 V 60 Hz 2 6 A 200 W ...

Page 37: ...id volume Description Value 1 unit stacked 9 L 2 units stacked 10 L 3 units stacked 11 L Top and bottom cooling 1200 W Power supply Voltage Frequency Power Consumption Rating 230 V 50 Hz 2 8 A 650 W 230 V 60 Hz 3 5 A 800 W 115 V 60 Hz 5 A 800 W Cooling liquid volume Description Value 1 unit stacked 9 L 2 units stacked 10 L 3 units stacked 11 L External cooling Description Value Pressure inlet 2 4 ...

Page 38: ...ials or quality levels is in the responsibility of the user CAUTION Danger of damage of property by released or broken flasks when using inapplicable flasks Flasks of bad quality or deficient contact surface may be accidentally released from the adhesive matting when shaking or may break at removing process Therefore Use only recommended flasks Use only flasks that are intact and free of scratches...

Page 39: ... mL 250 rpm Max rotations Erlenmeyer flasks Polycarbonate 125 3000 mL 200 rpm Max rotations Fernbach flasks Polycarbonate 3000 mL 250 rpm Type 25 mm throws Description Value Temperature range 16 60 C Size 200 x 200 mm Max rotations Erlenmeyer flasks Borosilicate glass 3 3 25 750 mL 250 rpm 1000 mL 300 rpm 2000 mL 300 rpm 3000 mL 350 rpm 5000 mL 300 rpm Max rotations Erlenmeyer flasks Polycarbonate...

Page 40: ... method capacitive Control method Inlet valve Hose 8 mm Legris connection Water consumption reference value 10 g h at 37 C 80 rH per unit Water quality Hardness 0 01 mmol L CaCO3 equivalent Conductivity 20 µS cm Dissolved solids 10 mg L Approachable values of humidity Temperature Maximum humidity 27 C 85 rH 33 C 85 rH 37 C 85 rH Path through Description Value Unit Roxtec EzEntry 4 Mini Diameter pa...

Page 41: ...ower consumption 135 mA Operating temperature 0 up to 50 C Measure accuracy 2 FS Control accuracy 0 001 Ln min Gassing ShakerBag Option 20l disposable bag Air Description Value Inlet pressure Max 0 5 bars Supply constantly Connection 8 mm Hose Pressure resistant Flow 2 00 Ln min Lead time 60 min Inner diameter exit gas hose Ø 14 5 mm ...

Page 42: ...5 bars Supply constantly Connection 8 mm Hose Pressure resistant Flow 1 00 Ln min Lead time 60 min Inner diameter exit gas hose Ø 14 5 mm 2l disposable bag Air Description Value Inlet pressure Max 0 5 bars Supply constantly Connection 8 mm Hose Pressure resistant Flow 0 20 Ln min Lead time 60 min Inner diameter exit gas hose Ø 14 5 mm ...

Page 43: ... Not possible CO2 gassing Gassing unit Description Value Inlet pressure Maximum 0 5 bars Supply constantly Flow clocked 120 480 NL h rotameter recommended Consumption Approx 13L h reference value Warm up period 15 min Temperature range Up to 60 C Humidity range 0 100 rH not condensing Connection inner diameter hose 6 mm Hose Pressure resistant Measuring range 0 20 CO2 Control range 0 19 5 CO2 Resp...

Page 44: ... mbars Long term stability 5 FS 2 years CO2 curtain Description Value Material PVC UV stabilized Heat resistance Up to 65 C CO2 pressure reducing unit Description Value Inlet pressure max 20 bars Supply constant Rotameter Flow 1 6 7 NL min Accuracy 4 FS Loss of pressure max 20 mbar Gassing tube Description Value Outer diameter Ø 10 mm Length 330 mm Control none ...

Page 45: ...e connection 115V 230V Life cycle 8000h FS 22 Length 438 mm Photosynthesis Fig 5 Description Value Fluorescent lamp T8 socket G13 15 Watts Color temperature 8500 K Life cycle 9000 h Starter single connection 115V 230V Life cycle 8000h FS 22 Length 438 mm Illumination algae Daylight Fig 6 Description Value Fluorescent lamp L 36 77 socket G13 36 Watts Luminous flux at 25 C 1400 lm Length 1200 mm Lif...

Page 46: ...s Wave length UV C 200 280 nm Maximum emission 253 7 nm Life cycle 9000 h Output UV C radiation 1 1 Watts Intensity after 5000 h 80 Output total 11 Watts Power consumption total 0 18 A 3 5 Operating conditions Description Value Ambient temperature max With without cooling 30 C Ambient temperature max without cooling 8 C below required minimum incubation temperature with cooling side cooling 15 C t...

Page 47: ...uid Secondary circuit Top cooling External cooling Permitted for food and pharmaceutical fields Freezing 40 C Corrosion copper 0 6 g m 2 Temperature range 40 C up to 150 C Water quality direct steam humidification Distilled water Deionized water Softened water Reverse osmosis water for details see appendix Cleaning agents generally Mild cleaning agents Disinfectants generally Quaternary ammonium c...

Page 48: ...de underneath the front door It contains the following information Fig 9 Name and internet address of the manufacturer TYPE Model Type NR Serial number VOLT Nominal voltage AMP Current consumption YEAR Year of manufacture CE marking 3 9 Interface communication protocol For communication with other equipment a serial interface is available The communication protocol can be ordered via info infors h...

Page 49: ...e not part of the delivery of the standard machine 4 1 Base unit Housing Fig 10 1 Drain hole 2 Ventilation holes 3 Mains connector with fuses 4 Blind opening 5 Main switch 6 Ventilation holes 7 Housing 8 Door handle 9 Operating panel 10 Door 11 Identification plate The incubator shaker is constructed as an insulated plastic housing with a sealed floor pan and a door 1 2 3 4 5 6 7 8 9 10 11 ...

Page 50: ...or Ventilation holes Door Fig 11 The following elements are located on the door 1 Window 2 Roller bearings 3 Glide rails 4 LED for incubation chamber Incubation chamber The walls of the incubation chamber are made of stainless steel Fig 12 The following elements are located inside the incubation chamber 1 Capillary thermostat with temperature controller 2 Ventilation outlets 2 cross flow fans with...

Page 51: ...2013 02 19 Page 51 of 185 There is a safety fuse located between the cross flow fans behind the rear wall It protects against overheating Fig 13 1 Table 2 Cones to lock the tray 3 Stop bar Fig 14 1 Rear stops 2 Guide rails Fig 15 Locking hooks 2 1 2 1 3 ...

Page 52: ...ge 52 of 185 2013 02 19 The following elements are located underneath the table Fig 16 1 Tray ejector 2 Drive belt 3 Counterweight Fig 17 4 Drain hole Optional on rear wall of incubation chamber Fig 18 CO2 sensor on the left on the rear wall panel 1 4 2 3 ...

Page 53: ...g 19 Humidity sensor on the right on the rear wall panel Fig 20 Mobile Pt100 sensor G Transport lock Fig 21 The table and the counterweight are secured with a star knob screw during transport to stop them moving uncontrollably This lock is located in the front section of the table ...

Page 54: ... The loading weights depend on the position of the deck in the stack the throw and the rotation speed NOTICE The loading weights and maximum speeds are given in the chapter Technical Data Temperature control The temperature is controlled via two cross flow fans which are each equipped with a downstream heating element The temperature is measured and controlled with a Pt100 temperature sensor The t...

Page 55: ...e rails and the embedded roller bearings located on the interior side of the door 2 Cylindrical pins stops in the glide rails limit the end position of the extended tray The fully extended tray rests partly on the door and partly on the table The table is stopped as soon as the door is opened by more than 30 The door can only be opened completely after the table has stopped moving The table restar...

Page 56: ...ically analog signal Visual alarm Value below setpoint Low Value above setpoint High Restart after power failure Acoustic alarm PIEP PIEP PAUSE PIEP PIEP An error message is triggered when the incubation shaker ha a fault This is the case when a component is defect or communication between sensor and control is interrupted Visual error message The displays show various error messages Please refer ...

Page 57: ...s alarm or error is triggered The warning symbol supports the user to operate the incubation shaker properly 4 4 Serial interface The unit is equipped with an RS 232 serial interface RS 232 9 PIN Fig 22 PIN assignments 1 6 NORMAL STATE 2 RX RECEIVE 1 7 ALARM and POWER FAILURE 3 TX TRANSMIT 8 RESERVE 4 RESERVE 9 RESERVE 5 GND Alarm contacts Fig 23 1 2 3 4 5 6 7 8 9 ...

Page 58: ...tion Analog Interface The unit is equipped with an analog interface DB 15 DB 15 female 15 PIN Fig 24 PIN assignments 15 GND 8 RESERVE 14 GND 7 RESERVE 13 GND 6 RESERVE 12 GND 5 RESERVE 11 GND 4 CO2 0 10 10 GND 3 HUMIDITY 0 100 9 GND 2 TEMP 0 100 C 1 RPM 0 500 RPM Signal strength 0 10V ...

Page 59: ... Page 59 of 185 4 6 Option bases The incubator shaker can be equipped with the following bases Fig 25 Rubber feet 3 cm bench top model standard Fig 26 Trolley base frame 13 cm plus castors optional Fig 27 Base frame 13 cm optional Fig 28 Base frame 31 cm optional ...

Page 60: ...anel comprises the following elements Function keys to enter parameter values Displays for parameters values messages and unit selection With the upper group of function keys parameter temperature CO2 Illumination and phase 1 are operated With the lower group of function keys parameter RPM Humidity O2 and phase 2 are operated Selection of parameters is operated with the scroll function key Abb 29 ...

Page 61: ...s parameters and units Displays messages Displays timer functions Displays Setpoint High Low 3 Display unit selection and remote access Parameter symbols and units lit orange when active Values and messages in the displays lit orange when active Warning symbol and warning messages lit red when active Function of timer lit blue when active Setpoint High and Low lit white when active ...

Page 62: ...rature can be set Humidity symbol Indicates that the humidity can be set Gassing symbol Indicates that the gassing can be set Illumination symbol Indicates that the illumination can be set Remote symbol Indicates that the unit is being set externally Setpoint symbol Indicates that a parameter can be set High symbol Indicates that a current parameter value is too high Low symbol Indicates that a cu...

Page 63: ...se 2 Time 1 symbol Indicates that programming mode of timer is active and Time 1 is selected Time 2 symbol Indicates that programming mode of timer is active and Time 2 is selected EXTERN symbol Indicates that the equipment is accessed by remote RESTARTED symbol Indicates that the equipment had a power supply interruption ALARM symbol Indicates that a current value of a parameter is in the alarm l...

Page 64: ... 4 digit display shows setpoints and current values of the temperature parameter CO2 parameter Light values of the time phase 1 warnings and error messages and informations of the equipment Lower display The 4 digit display shows setpoints and current values of the RPM parameter humidity parameter O2 parameter values of the time phase 2 warnings and error messages and informations of the equipment...

Page 65: ...mode of parameters or timer Option function key Scrolls through the installed parameters PLUS function key Increases the value of the selected parameter or time phase MINUS function key Decreases the value of the selected parameter or time phase TIMER function key Activates the edit mode of the timer REMOTE function key Switches between the controls systems of the individual units in stacked syste...

Page 66: ...0 The cooling unit is located in a housing mounted on top of the incubator shaker It can cool up to three incubators The cooling unit has a separate power supply The mains switch is located on the left hand side of the mounted housing The cooling unit has a twin circuit system with a coolant tank The coolant tank is located in the mounted housing Side mounted cooling system 380 watts Fig 31 The co...

Page 67: ...ling register must be connected to the external coolant system with a bypass Fig 32 The cooling unit is supplied with cooling liquid from the in house cooling system A control valve opens when necessary to allow coolant to flow through the cooling register The inlet and outlet of the coolant circuit are each equipped with a male hose nozzle with an outside diameter of 10 mm They are located on the...

Page 68: ...ks These stickers must not be removed under any circumstances 4 10 Option Direct Steam Humidification The incubator shaker is equipped with a Direct Steam Humidification The Direct Steam Humidification is switched on or off via the operating panel A humidity sensor located on the rear wall of the incubation chamber measures the humidity Please note the following points The Direct Steam Humidificat...

Page 69: ... mounted on the rear of the housing A controlled pump feeds water dropwise into an evaporator chamber via a feed hose The steam is then fed directly into the incubation chamber There are two types of water supply unpressurised from a water tank pressurised to max 0 3 bars from a pressurised pipeline 4 11 Option CO2 gassing The incubator shaker is equipped with a CO2 gassing unit This comprises A c...

Page 70: ... housing The CO2 supply is provided with a control valve There is a CO2 sensor inside the chamber that transmits the measured CO2 content to the control unit The CO2 gassing unit is operated via the operating panel Transparent safety curtain Fig 36 1 PVC safety curtain 2 Circulation holes for humidification optional 3 Snap fasteners 4 Marking Fig 37 Snap fastener 4 1 2 3 ...

Page 71: ...f the tray The curtain is marked outside so that it can be fitted correctly The transparent curtain is attached to the upper front edge of the housing by means of the 4 snap fasteners The curtain prevents large amounts of CO2 escaping when the door is opened NOTICE The safety curtain keeps the CO2 gas inside the incubation chamber approx 60 on opening the door approx 40 on pulling out the tray The...

Page 72: ...onsists of an adjustable rotameter with a control range of 120 480 Nl h Fig 42 The adjacent diagram shows the gas feed cycle for the CO2 supply Effective flow rate Mean flow rate 4 13 Optional Illumination A unit with sockets for fluorescent tubes is mounted on the ceiling of the incubation chamber NOTICE Owing to the diminished height of the incubation chamber the maximum volume of the culture fl...

Page 73: ...be used daylight tubes photosynthesis tubes Fig 43 The illumination unit is mounted on the ceiling of the incubation chamber The tubes have sockets at both ends G13 The tubes can be switched on and off in pairs as required Fig 44 This is carried out using the switch located on the ceiling between the switchable tubes ...

Page 74: ...nd left hand sides of the housing The illumination is switched on and off using the operating panel of the incubator shaker Fig 45 The walls of the incubation chamber have a white coating The illumination is equipped with a dimmer Fig 46 The inside of the door is lined with a white panel It is used to exclude light and improve light distribution within the incubation chamber Fig 47 A Plexiglas pan...

Page 75: ...sture and gases when hoses or cables are routed into the incubation chamber Fig 50 The pass through consists of divisible elements that can be removed in layers to adjust the opening to fit the diameter of the hose or cable see chapter Installation and initial operation Fitting the hose cable pass through A red cylindrical plug and a bag of Roxtec lubrication gel are included to the shipping Close...

Page 76: ...ear wall of the incubation chamber It can be used in a reagent flask underneath disposable culture bags During operation the mobile temperature sensor must be placed in a reference flask containing water or if incubation is carried out in a disposal culture bag it must be placed in its intended position underneath the bag The temperature is controlled automatically in the cascade mode i e the valu...

Page 77: ...t with moving parts Please note the following points If a mobile temperature sensor is installed it must always be immersed in liquid in the reference flask or placed in its intended position underneath a culture bag When the mobile temperature sensor is inserted into its holder it measures the air temperature This may cause cycling of the temperature control system If the mobile temperature senso...

Page 78: ...table to reduce vibrations during operation If the tray weight is more than 12 kg loaded including holders and at higher speeds the counterweights can be set to the position HL heavy load The positions for mounting the two weights are marked Throw 12 5 HL 25 Throw 25 HL 50 Throw 50 Fig 53 Underneath the table there is a prop on the left hand tray ejector It is used to secure the table in the highe...

Page 79: ...UV radiation with a wavelength of 253 7 nm The air drawn in by the cross flow fans is fed through this light trap The UV radiation kills any microorganisms in the air supply Fig 54 The picture on the left shows the position of the UV sterilisation units behind the stainless steel lining of the incubation chamber The UV sterilisation units are not visible Fig 55 The switch for the air sterilisation...

Page 80: ...ransportation Remove packaging finally only when the equipment is ready to be brought into use 5 2 Transport inspection Immediately verify delivery on receipt for completeness and freedom from any transit damage Proceed as follows when transit damage is physically identifiable Do not accept delivery or under reserve only Note extent of loss on transport documentation or delivery note Initiate comp...

Page 81: ...stroy the packaging and do not remove it just before starting initial operation 5 4 Transport CAUTION Risk of damage due to extreme localised loads An extreme localised load may cause the feet to shear off or leave dents in the base plate Therefore Never push the unit Owing to its weight the incubator shaker must never be transported or moved by one person alone If the unit has to be relocated ove...

Page 82: ...ecause of an eccentric balance point 4 Lift the packed item and start to transport it 5 5 Storage of the incubator shaker The unit must be stored as follows Do not store outdoors Do not expose to aggressive substances Do not expose to sunlight Avoid mechanical vibrations and shocks If stored for more than 3 months make regular checks of the general condition of the unit and its packaging If necess...

Page 83: ...tion and initial operation are to be carried out by the manufacturer s associates only Call the manufacturer when any subsequent translocation is foreseen Prevent arbitrary installation and translocation 6 1 Safety Personnel Installation and initial operation may only be carried out by qualified personnel Electrical system DANGER Danger of fatal electric shock Contact with electrically powered com...

Page 84: ...lly Secure component parts so that they cannot fall off or tumble 6 2 Location requirements for installation 6 2 1 Place of installation The following requirements must be met for the installation of the unit The equipment must be installed and operated inside a building The unit must not be exposed to direct sunlight NOTICE The incubator shaker must be protected against hot exhaust air of other e...

Page 85: ...ashed water Protect the outside of the unit against extreme levels of dust and dirt NOTICE Any other type of installation must first be checked with the manufacturer and requires the written permission of the manufacturer 6 2 2 Minimum distances The incubator shaker must be set up as follows for operation handling and repair work NOTICE Main switch and cable for power supply must be easily and fre...

Page 86: ... 86 of 185 2013 02 19 6 3 Removing the transport lock Before the unit can be put into operation the transport lock must be released and removed Procedure Fig 57 1 Release and remove the star knob screw 2 Store the screw and the supplied tools together for future transports ...

Page 87: ...ozzle Before commissioning the supplied hose nozzle must be screwed into the drain hole Procedure Fig 58 1 Unscrew the blanking plug from the drain hole Fig 59 2 Screw in the hose nozzle NOTICE To drain condensate or rinsing fluids mount one end of a hose onto the nozzle and place the other end in a suitable container ...

Page 88: ...t OFF switch is not illuminated Unit ON switch is illuminated CAUTION Risk of material damage if the door is forced downwards when the unit is switched off The door mechanism may sustain damage if it is forced completely open when the incubator shaker is switched off Therefore Do not force the door completely open when the incubator shaker is switched off NOTICE Frequent ON OFF switching of the un...

Page 89: ...e to uncontrolled movement If the unit has not been leveled properly it may move around uncontrollably if it starts to vibrate Therefore If the unit shows any signs of vibration or unusual movements level it again Rubber feet The bench model cannot be leveled out It must stand on a firm flat surface Trolley NOTICE The trolley cannot be leveled out Remove both front castors out of the frame before ...

Page 90: ...edure Fig 62 1 Release the locknut of the leveling foot 2 Set the shaking speed of all units in the stack to 50 rpm 3 Start the shakers 4 Check the stacked units for vibrations or uneven operation 5 In the event of vibrations or uneven operation adjust the levelling foot upwards or downwards with an open end wrench 19 mm until the stacked units operate smoothly 6 Check the smooth running of the un...

Page 91: ...5 C The absolute tolerance of the thermostat is 2 5 C Procedure 1 Set the thermostat to 70 C 2 Shut the door of the unit 3 Switch on the unit 4 Press option key to select the temperature parameter The temperature parameter lights up 5 Press Set key Setpoint lights up 6 Press the PLUS key until the maximum operating temperature e g 50 C is set 7 Press the ON OFF key to switch on the temperature con...

Page 92: ...fied setpoint shown in the display 6 8 Setting the adjustable counterweight The counterweight with a variable throw is equipped with two detachable weights that can be flexibly mounted These weights are positioned according to the throw and the load The throw can be altered with an adjustable axle CAUTION Risk of material damage due to use of too high shaking speed The incubation shaker may move d...

Page 93: ...stallation and initial operation 2013 02 19 Page 93 of 185 6 8 1 Changing the throw Change the throw as follows Procedure Fig 63 1 Remove the 4 Allen screws from the table Fig 64 2 Lift the table Fig 65 3 Support the table with the prop ...

Page 94: ...djustable axle Fig 67 5 Remove the 4 Allen screws from the adjustable throw axle Fig 68 6 Insert the throw axle into the desired throw position 12 5 mm 25 mm 50 mm The marking on the edge of the throw axle must line up with the marking on the counterweight The different throws are engraved on the counterweight 7 Attach the adjustable throw axle to the counterweight using the 4 Allen screws ...

Page 95: ...e counterweights Procedure Fig 69 1 Remove the Allen screws securing the detachable counterweights 2 Remove the detachable weights from the counterweight 3 Insert the cylindrical pins in the desired counterweight positions 4 Place the detachable counterweights in their desired positions on the counterweight 5 Screw in the Allen screws to secure the detachable counterweights Fig 70 6 Hold the table...

Page 96: ... if the ambient temperature at the cooling aggregate is below 30 C Ensure sufficient air circulation See chapter Installation Side mounted cooling system The side mounted cooling system can be put into operation without any previous preparatory work External cooling system The cooling register must be connected to the external cooling system via a feed hose and a return hose Top mounted cooling sy...

Page 97: ...AUTION Risk of material damage due to dry running of the pump and insufficient cooling If cooling systems with a coolant circuit top mounted or base cooling systems are not filled with the supplied coolant before initial operation this will have a negative effect on the cooling efficiency and will damage the pump if it runs dry Therefore Before initial operation fill the coolant system with the se...

Page 98: ... system must be filled with the supplied cooling liquid before it is put into operation Auxiliary tool Funnel Procedure Fig 73 1 Remove the cap from the filler port of the coolant tank 2 Take the cooling liquid canister out of the incubator 3 Place the funnel in the filler port Fig 74 4 Pour in the cooling liquid 5 Replace the cap of the filler port ...

Page 99: ...o use an antifreeze The male nozzles for the inlet and outlet of the external coolant system are labelled The labels are located on the top panel or on the rear wall of the incubator shaker Fig 75 Label of cooling liquid inlet Fig 76 Label of cooling liquid outlet Connect the in house cooling system as follows Procedure Fig 77 1 Push the cooling feed hose onto the male inlet nozzle 2 Secure the co...

Page 100: ...tap water Therefore Use only the recommended types of water for humidification Before starting initial operation the Direct Steam Humidification it must be connected to an unpressurised water tank or to a pressurized water supply The inlet pressure must not exceed 0 3 bars NOTICE Place the water tank on the floor to avoid damaging the equipment and the electronics The water tank must fulfil the fo...

Page 101: ...d hoses must be mounted on the Schott connectors as follows Direct mounting of hoses Procedure 1 Cut the end of the hose cleanly Fig 79 2 Insert the hose as far as it will go into the quickfit connector Mounting the hose nozzle plus hose Instead of mounting the hose a hose plus a male nozzle can be inserted into the Schott quickfit connector Procedure 1 Insert the male hose nozzle into the Schott ...

Page 102: ...ts Requirements for air gas supply Constancy of the air gas supply The air gas must be clean dry oil and dust free Requirements for the hose Only pressure resistant and intact hoses may be used The hose must have an appropriate diameter an adapter may be used if necessary Secure hoses with hose clamps 6 14 Connecting the gassing pipe The in house supply of air or gas to the unit must meet the foll...

Page 103: ... biology or bio technology technicians Inappropriate operation WARNING Risk of injury due to inappropriate operation Inappropriate operation may lead to severe physical injury or loss of property Therefore Strictly follow the instructions stated in this operating manual CAUTION Risk of burns on touching hot surfaces There is a risk of burns from hot surfaces if the table and or cultivation flasks ...

Page 104: ...til this phase is complete The initialization information disappears automatically when the initialization is complete Both displays show the version of the control type e g Std for standard Following the version of firmware is displayed e g Firmware 3 02 Finally the access mode for the service mode is active for a few seconds for authorized personnel only Displays change to operating mode subsequ...

Page 105: ...it is operated externally the EXTERN sign lights up The unit can be operated externally using any of the operating panels on the stack of units Fig 81 Display area In the display area there are two alpha numeric displays with 4 characters parameter signs function signs and unit symbols Alpha numeric display with 4 characters This displays show parameter values alarm messages error messages and uni...

Page 106: ...s the selected parameter or timer on or off TIMER Activates the timer mode Following functions are operable via the operating panel Switching on the interior light The interior light is switched on by pressing any key once It turns off automatically after 1 minute Switching on parameters The installed parameters are selected by repeatedly pressing the option key If the parameter is switched off th...

Page 107: ...ly with the next biggest increment Increments RPM 1 RPM 10 RPM Temperature 0 1 C 1 C Humidity 0 1 rH 1 rH CO2 0 1 1 Timer 1 min 10 min 30 min 1 h 12 h NOTICE The setpoint values of the parameters do not have to be confirmed They are accepted after entry without confirmation The parameter can be switched on with the ON OFF key The control system returns to the operation mode when no key is pressed ...

Page 108: ...he timer are switched on the incubation shaker alters between operating mode of Phase 1 and Phase 2 with the set time interval If Time 1 and Time 2 are switched on the cycle symbol lights blue 7 4 Operating the timer 7 4 1 Displaying the time In the upper display the time of Phase 1 Time 1 is shown In the lower display the time of Phase 2 Time 2 is shown The duration will be displayed as follows D...

Page 109: ...n be set with the TIMER key In operating mode Time 1 all parameters are set that are active in Phase 1 In operating mode Time 2 all parameters are set that are active in Phase 2 NOTICE Do not activate the timer in Time 2 This starts the programmed cycle CAUTION Using the Sticky Stuff adhesive matting there is a risk of loss of property due to condensation caused by sudden drops in temperature Do n...

Page 110: ... of the cultivation parameters after a specific time Fig 82 For setting the timer for a single change proceed as follows Procedure 1 Press TIMER key Time 1 and Time 2 light up blue To operate Time 1 use the upper function keys To operate Time 2 use the lower function keys 2 Press SET key Setpoint at Time 1 lights up 3 Press PLUS key to set time for Phase 1 ...

Page 111: ... NOTICE For a single changeover the timer must be switched off in Phase 2 9 Press option key to confirm programming The shaker now operates until end of preset time in operating mode of Phase 1 Function symbol TIMER lights blue and Phase 1 blinks blue After end of Time 1 operating mode of Phase 2 is active Function symbol TIMER does not light Operating mode Phase 2 blinks blue The unit is operatin...

Page 112: ...ltivation parameters after specific times Fig 83 For setting the timer for a cycling change proceed as follows Procedure 1 Press TIMER key Time 1 and Time 2 light up blue To operate Time 1 use the upper function keys To operate Time 2 use the lower function keys 2 Press upper SET key to set time for Phase 1 Setpoint at Time 1 lights up 3 Press upper PLUS key to set time for Phase 1 ...

Page 113: ...oint at Time 2 lights up Press lower PLUS key to set time for Phase 2 5 Press lower ON OFF to switch Time 2 on 6 Press option key to select parameters of Phase 1 Phase 1 lights up 7 Press SET key 8 Press PLUS or MINUS key to set setpoint 9 Press ON OFF to switch on parameter 10 Press option key to select parameters of Phase 2 Phase 2 lights up 11 Press SET key ...

Page 114: ...ime phases are active the cycle symbol lights up Settings are saved now Operation mode of Phase 1 and Phase 2 are blinking when they are active 7 4 5 Display the remaining runtime of the timer The following timer modes can be displayed during the first timer interval Phase 1 of the incubation process Remaining runtime by pressing the TIMER key Remaining runtime by pressing the TIMER key and then p...

Page 115: ...unit individually or all units NOTICE The units in a stack are assigned from the bottom to top Symbol for selection a unit The symbol on the operating panel for selecting units shows that all 3 units are selected for remote access External operation of a unit NOTICE The timer function cannot be operated from remote Units that are under operation of the timer cannot be accessed by remote All units ...

Page 116: ...RN and the warning symbol light up Set parameters of incubation shaker see chapter Operation Starting a working cycle Displays of the remote accessed incubation shaker show the values and parameters of the unit from which the remote access occurs 2 Press option key to select parameter 3 Press SET key 4 Press PLUS or MINUS key to set setpoint ...

Page 117: ...ght up on the operating panel NOTICE Ensure that the flasks shaken are fixed securely Watch flask from the start of the shaking operation until setpoint is reached Procedure 1 Press option key to select parameter E g Parameter temperature is selected Temperature sign and unit C light up If parameter is ON upper display shows the current value e g 30 0 C If parameter is OFF upper display shows OFF ...

Page 118: ...ayed in the upper display If no keys on the operating panel are pressed within 2 seconds the display alternates between the setpoint value und the operating status OFF 4 Press ON OFF key to switch parameter on Parameter sign lights up Select next desired parameter If no key is pressed within 2 seconds The upper display changes to the current value of the parameter e g 30 0 C If this value is above...

Page 119: ...o select illumination Parameter Light lights up 3 Press SET key 4 Press ON OFF key to switch on parameter illumination Alternating display All parameters will be shown in the displays alternatingly The display shows current value if parameter is switched on The display shows OFF if parameter is switched off Static Temperature sign lights Upper display shows current value if switched on If paramete...

Page 120: ...utomatic start up of the incubator shaker If a work cycle is incorrectly terminated by switching off the unit with the main switch the unit can restart automatically when it is switched on again This may damage the incubator shaker and the cultures Therefore Never terminate a work cycle using the main switch of the unit Do not leave any objects in the incubation chamber when the unit is switched o...

Page 121: ...ioning of the alarm relay as follows Procedure 1 Open the door 2 Set capillary thermostat to a low value e g 30 C 3 Shut the door 4 Press option key to select temperature parameter 5 The temperature parameter lights up 6 Press SET key Setpoint lights up 7 Press the PLUS key to set the value of the temperature parameter above the capillary thermostat e g 40 C 8 Press the ON OFF key to switch on the...

Page 122: ...t too low Set capillary thermostat to higher temperature Operator Temperature is too high Deviation 1 C Cooling is not switched on Switch on cooling Operator Fan is not operating Inform Infors representative Qualified technician Setpoint is below the technically feasible value Increase setpoint value Operator Speed is too low Deviation 10 rpm Tray is too heavily loaded Load tray properly Operator ...

Page 123: ... high Reduce the temperature setting Operator Humidity is too high Deviation 10 Temperature is too low Increase the temperature setting Operator Open door to let wet air escape Operator Power failure No actions required Unit was switched off with activated parameters No actions required 7 11 Operating the tray CAUTION Risk of damaging the door and the door mechanism if the opened door is subjected...

Page 124: ...ust click into place behind the front stop Fig 85 4 Check whether the tray sits behind the front stop Closing the door lowers the tray ejectors and thus lowers the tray into the two cones At the same time the locking hooks move forwards to secure the tray to the table CAUTION Risk of material damage if the tray is not locked If the tray is not secured to the table by the locking mechanism the tray...

Page 125: ... speed rpm or by half opening the door and waiting until the table has come to a complete stop 2 Open the door to its full extent The safety mechanism automatically disengages the hooks when the door is opened and lifts the tray out of the cones at the rear NOTICE If the tray cannot be pulled out check whether the door is completely open Press the door down to its full extent 3 Lift the tray by th...

Page 126: ...roblem and the door cannot be completely opened the tray can also be removed manually by two people if possible Procedure 1 Unload the tray 2 Open the door as far as possible Fig 87 3 Person 1 Press both hooks of the locking mechanism backwards 4 Person 2 Lift the tray out of the locking mechanism 5 Pull the tray over the front stop on the table ...

Page 127: ...of 80 NOTICE The tray is disengaged when the door is opened by more than 80 3 Handle the flasks 4 Close the door The incubator shaker automatically starts operating again with the preset parameters 7 13 Loading the trays CAUTION Risk of material damage due to excessive wear resulting from uneven loading of trays Uneven loading of trays considerably shortens the service life of the suspension mecha...

Page 128: ...ps 3 Load the tray evenly 4 Position any optional measuring sensors 5 Push the tray back all the way to the stop 6 Close the door NOTICE If the tray has a low load it is advisable to place a few flasks filled with water on the tray This will help the table to run more smoothly The incubator shaker is designed for an average load of 12 kg The permissible tray loads are given in the chapter Technica...

Page 129: ...ing humidification Loading the Sticky Stuff adhesive matting The Sticky Stuff adhesive matting is used underneath cultivation flasks with large flat bottoms Only undamaged unscratched cultivation flasks may be used on the adhesive matting To achieve maximum adhesion to the Sticky Stuff adhesive matting the flasks and matting must be completely undamaged clean dry and oil free After placing the fla...

Page 130: ...can be covered with the supplied protective film 7 15 Interrupting operation The unit can be stopped by opening the door All functions and parameters remain activated The door can only be fully opened once the table has stopped moving NOTICE The door must be held half open while the table is slowing down The door lock is only disengaged once the table has stopped moving provided that the door is n...

Page 131: ...ilisation ON push button switch is illuminated 7 17 Operating the Direct Steam Humidification NOTICE The cooling system switches off automatically when the direct steam humidification is switched on The Direct Steam Humidification is switched on using the operating panel Procedure 1 Press the option key to select the illumination parameter Parameter sign and unit light up 2 Press SET key 3 Press P...

Page 132: ... system 900 watts 1200 watts The cooling system has its own power supply The cooling system is switched on with the green push button on the left hand side of the cooling module The push button lights up when the cooling system is switched on Side mounted cooling system 380 watts The incubation shaker supplies power to this cooling system The cooling system is ready for operation when the incubati...

Page 133: ... MINUS key to set setpoint 4 Press ON OFF key to switch parameter on The upper display shows ON and then the current value of the parameter e g 18 5 C 7 19 Starting illumination The illumination is switched on using the operating panel This requires that the switches for the individual pairs of tubes are turned on NOTICE The fluorescent tubes generate heat during operation Therefore always switch ...

Page 134: ... key 3 Press ON OFF key to switch parameter on The display shows ON The fluorescent tubes can be turned on or off in pairs with a push button The fluorescent tubes are connected pairwise from outwards to inwards Fig 89 Switch and tube pair 1 Switch and tube pair 2 Switch and tube pair 3 Switch and tube pair 4 Switch and tube pair 5 Switch and tube pair 6 ...

Page 135: ...arameter sign Light lights up 2 Press SET key 3 Press ON OFF key to switch parameter on Display shows ON 4 Set the light intensity with the rotary knob 7 21 Operating the CO2 gassing system NOTICE To prevent excessive consumption of gas the door drain hole and path through optional of the incubation shaker must be closed NOTICE The CO2 gassing system only works correctly when the temperature contr...

Page 136: ...o change setpoint 4 Press ON OFF key to switch on parameter Display shows current value e g 5 0 Using the safety curtain The safety curtain is attached to the ceiling of the incubation chamber with four snap fasteners NOTICE The label Outside must face outwards Fig 90 Detaching the safety curtain Undo each snap fastener with a gentle tug Attaching the safety curtain Gently press the two halves of ...

Page 137: ...assing pipe is an opening for the gas supply The pressure and flow rate must be selected by the operator for the particular application NOTICE The temperature control system must be switched on to ensure uniform distribution of gas in the incubation chamber 7 24 Operating the mobile Pt100 sensor The temperature can only be accurately measured with the mobile Pt100 sensor using a reference flask NO...

Page 138: ...the Pt100 sensor in the liquid inside the reference flask 4 Secure the cable of the Pt100 sensor with a plug of cotton wool in the neck of the reference flask 7 25 Setting up the pass through Fit hoses or cables as follows Procedure Fig 92 1 Remove the knurled screws holding the frame 2 Remove the frame and blue modules from the housing Fig 93 3 Press the modules out of the frame Fig 94 4 Split th...

Page 139: ...moved segments in the supplied bag They are used to reseal the pass through Fig 96 The halves of the modules must fit together to leave a 0 1 to 1 0 mm gap between them This ensures that the fitted bushing seals off the incubation chamber Fig 97 6 Insert the hose cable through the opening in the frame 7 Grease the inner surface of the frame and module halves with installation gel ...

Page 140: ... through the opening in the housing Fig 99 11 Secure the frame to the housing with the knurled screws CAUTION Risk of material damage due to abrasion and trapping of hoses and cables Hoses and cables may be damaged if they are trapped during shaking or if they are chafed by parts inside the chamber Therefore Position cables and hoses so that they cannot come into contact with anything ...

Page 141: ... be worn when carrying out certain work is indicated separately in this section Maintenance work carried out inappropriately WARNING Risk of injury due to maintenance work carried out inappropriately Maintenance work carried out inappropriately may lead to severe physical injury or loss of property Therefore Make sure that enough space is provided for the work before starting Keep the work site ti...

Page 142: ...zard WARNING Biohazard Noncompliance with biological safety regulations increases the health risk of the operator and the risk of lower equipment functionality Therefore Strictly follow all biological safety regulations Decontaminate and clean every part that comes in contact with any infectious biochemical substances WARNING Risk of harm to health due to contaminated component parts There is a ri...

Page 143: ...e following chapters Reduce the maintenance intervals in case increased abrasion is detected during regular checks Contact the manufacturer for questions concerning maintenance For contact details see page 2 For proper operation the incubation shaker temporarily needs following maintenance work Interval Maintenance work To be carried out by Before each use Check that the chamber lights are working...

Page 144: ...hnician Option Cooling System Check the cooling liquid level in the tank Top up if necessary Qualified technician Calibrate measuring devices Qualified technician Every 3 years Option Cooling System Replace cooling liquid of top bottom cooling Qualified technician As required Disinfect unit Operator Option Sticky Stuff Clean Sticky Stuff adhesive matting Operator Option Sticky Stuff Replace Sticky...

Page 145: ... until it clicks into place 8 5 Cleaning the cooling system The cooling fins of the condenser must kept free of dust to allow optimum heat dissipation This requires regular inspections and cleaning CAUTION Risk of damaging the heat exchanger due to the use of sharp objects The copper pipes of the heat exchanger may be damaged by cleaning with sharp objects thus leading to the escape of coolant The...

Page 146: ...ner 4 Reattach the grating with the two screws Top mounted cooling system Procedure Fig 102 1 Visually inspect the heat exchanger of the top mounted cooling system for dust 2 If there is a lot of dust contact the manufacturer s service center Coolant Maintenance of the coolant system must be carried out by the manufacturer s service center Annual check of the coolant level Replacement of the coola...

Page 147: ...escent tubes Replace defect fluorescent tubes as follows Procedure 1 Open the door 2 Switch off the unit 3 Rotate the tube by 90 Fig 103 4 Pull the defect tube downwards out of both sockets Fig 104 5 Push the new tube with the contact pins in a vertical position upwards into the sockets 6 Rotate the tube by 90 ...

Page 148: ...tain mercury Dispose of fluorescent tubes according to the local statutory regulations 8 7 Cleaning the algae illumination Clean the Plexiglas panel and the tubes as follows Procedure 1 Open the door 2 Switch off the unit and disconnect it from the power supply Fig 106 3 Hold onto the front edge of the Plexiglas panel with both hands and pull it downwards uniformly off the tubes 4 Clean the Plexig...

Page 149: ... 2 Push the new hose into the connector the O ring does not need to be pressed 8 9 Replacing the Sticky Stuff adhesive matting NOTICE Regular disinfection may reduce the adhesiveness of the Sticky Stuff matting Replace the adhesive matting as follows Procedure 0 1 Release the adhesive matting on one side of the tray and then pull upwards at an angle 2 Degrease the tray with acetone 3 Thoroughly mo...

Page 150: ...leaning rag Clean inside and outside of the glass door pane with a soft cleaning rag and mild detergent The following areas of the incubator shaker must be cleaned regularly Housing Chamber Floor pan Trays Cooling systems CAUTION Risk of material damage if the incubation chamber is not cleaned sufficiently Moulds or other microorganisms may grow uncontrollably and thus jeopardise successful cultiv...

Page 151: ...eaner If necessary disinfect with a standard disinfectant 8 11 2 Cleaning the chamber Wipe the housing regularly with a soft cloth and a mild household cleaner e g washing up liquid neutral cleaner If necessary disinfect with a standard disinfectant 8 11 3 Cleaning the floor pan When cleaning the floor pan after a glass breakage or if culture liquids have escaped the table can be released from the...

Page 152: ...pressurised water hose for cleaning If necessary 6 Rinse the floor pan with hot water and a mild household cleaner Fig 109 Rinsing fluids must be drained away through the drain hole at the side of the unit NOTICE If the drain is not connected to the in house wastewater system push a hose onto the drain nozzle and let the water run into an appropriately sized container 7 Rinse the floor pan with wa...

Page 153: ...ing up liquid Allow to dry overnight Disinfect with quaternary ammonium compounds CAUTION Risk of material damage due to the use of solvent based cleaning agents The structure and adhesive properties of the Sticky Stuff adhesive matting can be destroyed by solvent based cleaners Risk of detachment of cultivation flasks from the Sticky Stuff adhesive matting during operation of the shaker Therefore...

Page 154: ...ng up liquid neutral cleaner 3 If necessary disinfect with a standard disinfectant 8 14 Finishing maintenance work WARNING Risk of injury If protective devices on the equipment are not brought back to their original state after maintenance work this carries a substantial risk of injury at the next time of operation Therefore Always restore all protective devices on the equipment back to their orig...

Page 155: ...ces The following instructions are to be followed 1 Immediately switch off the equipment and unplug the mains plug in case of malfunctions which represent an imminent danger to persons or property NOTICE Instructions given by in house safety protocols are to be followed if additional isolation switches for the power supply have been installed locally 2 Investigate the cause of the malfunction 3 In...

Page 156: ...ud noises Smoke Odor Strong vibrations After switching off and disconnecting from the power supply proceed as follows Procedure 1 Avert danger 2 Remove flasks manually from the tray NOTICE If the incubator shaker is switched off the tray cannot be removed because the door lock cannot be disengaged 3 Remove the tray manually see chapter Operation Operating the tray NOTICE If the cultivation flasks ...

Page 157: ...anger of fatal electric shock Contact with electrically powered components is perilous Energised electrical components may perform uncontrolled movements which can lead to severe injuries Therefore Turn off the electrical supply and check whether it is electrically isolated before starting any work WARNING Risk of injury due to inappropriate trouble shooting Inappropriate trouble shooting may lead...

Page 158: ...ly opened Door is subjected to a load Close the door and open again Operator Unit is switched off Switch the unit on Operator Tray lock does not engage A foreign body is blocking the locking system Remove foreign body Operator Foreign body is under the table Detach table from its axle tilt it upwards and remove the foreign body Operator Tray is bent or damaged Replace the tray Operator Tray is not...

Page 159: ...act Infors representative Qualified technician Defect motor fuse Contact Infors representative Qualified technician Defect motor Contact Infors representative Qualified technician Motor not connected to electronics Contact Infors representative Qualified technician Shaker drive accelerates too quickly or operates too quickly Defect motor control unit Contact Infors representative Qualified technic...

Page 160: ...r flow rate Operator CO2 concentration is too high Deviation 1 CO2 inlet pressure is too high Reduce the inlet pressure Operator CO2 value is fluctuating current value is not constant Inlet pressure or flow rate is too high Reduce the inlet pressure or the flow rate Operator Temperature interference Interferences Possible causes Trouble Shooting By Temperature higher than 65 C Error message Defect...

Page 161: ...ative Qualified technician Temperature in flask with mobile Pt100 is too low 0 C Defect measuring electronics Contact Infors representative Qualified technician No value on temperature display Pt100 sensor or sensor cable is defect Contact Infors representative Qualified technician Temperature too high No error message Cooling is not switched on Switch on cooling Operator No power supply to coolin...

Page 162: ...or Capillary thermostat is set too low must be at least 10 C above the setpoint value Set capillary thermostat to higher temperature Operator Heating fan is not working Contact Infors representative Qualified technician Door is not completely closed Close the door properly Operator Top mounted cooling cooling fins are dusty Contact Infors representative Qualified technician Side mounted cooling co...

Page 163: ...servoir is empty Fill water reservoir Operator Air pocket between wet filter and reservoir Replace wet filter with a dry one Operator Valve is defect Contact Infors representative Qualified technician Transmission error of the humidity sensor or the temperature sensor Restart software switch unit OFF and then ON Operator Steam generator is defect Contact Infors representative Qualified technician ...

Page 164: ... is defect Contact Infors representative Qualified technician Illumination algae is not working Control is defect Contact Infors representative Qualified technician Daylight illumination is not working Illumination parameter is not activated Activating the illumination parameter Operator Tubes have not been turned on with the switch inside the chamber Switch on Operator Lamp ballast is defect Cont...

Page 165: ...pieces of tube and cleaning equipment so that they are airtight Ensure they are disposed of correctly If a fluorescent tube has broken proceed as follows Procedure 1 Switch off the unit 2 Disconnect the unit from the power supply 3 Remove fluorescent tube and shards out of the incubation chamber For optional illumination fluorescent tubes 4 Remove the fluorescent tube out of the lamp socket 5 Fit ...

Page 166: ...nt or the component part which is to be repaired must be entirely decontaminated before sending to the manufacturer The operator is obliged to firstly fill in and sign a decontamination agreement and decontamination form and either send it together with the written request for the repair work to the licensed dealer before returning the faulty equipment to the manufacturer or at the latest together...

Page 167: ...sassembly process ensure that there is sufficient space for the whole procedure Take care regarding sharp edged components Keep the work place tidy and clean Discarded components and tools may be a source of accidents Professionally disassemble units Take note of the high dead weight of some parts Use hoists if necessary Secure units to avoid them falling or tumbling down Call the manufacturer in ...

Page 168: ...that comes in contact with any infectious biochemical substances e g liquids which contain bacteria or viruses 10 2 Disassembly Prior to disassembly Switch off the equipment and lock off any isolation switch Physically disconnect the main energy supply from the equipment and wait for any components to fully discharge Remove and dispose of all additional consumable items auxiliary components and or...

Page 169: ...e of the remaining components according their material composition CAUTION Environmental damage or loss due to inappropriate disposal Electronic waste electronic components lubricants or other auxiliary materials supplies are subject to hazardous waste regulations and may only be disposed of by registered specialist disposal firms Local authorities or specialist disposal firms can provide informat...

Page 170: ... continuously with these parameters until other values are entered Procedure 1 Press TIMER key to start timer function Time 1 and Time 2 light up blue Operate Time 1 with the upper function keys Operate Time 2 with the lower function keys 2 Press upper SET key to edit timer settings Time 1 and Setpoint light up blue 3 Press PLUS key to set time to 10 minutes for Phase 1 4 Press ON OFF key to switc...

Page 171: ...9 Press ON OFF key to switch on parameter RPM 10 Press option key to select parameters of phase 2 Phase 2 light up blue 11 Press PLUS or MINUS key to set parameter RPM to 100 12 Press ON OFF key to switch on parameter RPM 13 Press option key to finish timer settings Current active phase blinks blue The incubation shaker starts with the settings of phase 1 After 10 minutes the speed slows down to 1...

Page 172: ...e been entered Procedure 1 Press TIMER key Time 1 and Time 2 light up blue Operate Time 1 with the upper function keys Operate Time 2 with the lower function keys 2 Press SET key for timer settings Setpoint and Time 1 light up blue 3 Press PLUS key to set Time 1 to 5 minutes 4 Press ON OFF key to switch on Time 1 Time 2 is switched off 5 Press option key to select parameters of Phase 1 Phase 1 lig...

Page 173: ...per PLUS or MINUS key to set parameter Temp to 37 5 C 9 Press ON OFF key to switch on parameter Temp 10 Press lower PLUS or MINUS key to set parameter RPM to 250 RPM 11 Press ON OFF key to switch on parameter RPM 12 Press option key to finish timer settings Current active phase blinks blue Incubation shaker starts after 5 minutes with current settings ...

Page 174: ...perates with two alternating phases Phase 1 Speed 250 rpm Temperature 40 C Time 10 minutes Phase 2 Speed 100 rpm Temperature 37 C Time 7 minutes The unit operates continuously with these parameters until other values are entered Procedure 1 Press TIMER key Time 1 and Time 2 light up blue Operate Time 1 with the upper function keys Operate Time 2 with the lower function keys 2 Press upper SET key f...

Page 175: ...Setpoint and Time 2 light up 6 Press lower PLUS key to set time to 7 minutes 7 Press ON OFF key to switch on Time 2 8 Press option key to select parameters Phase 1 lights up blue 9 Press upper PLUS or MINUS key to set parameter temp to 40 C 10 Press ON OFF key to switch on parameter Temp 11 Press lower PLUS or MINUS key to set parameter RPM to 250 12 Press ON OFF key to switch on parameter RPM ...

Page 176: ... parameter Temp to 37 C 15 Press ON OFF key to switch on parameter Temp 16 Press lower MINUS key to reduce parameter RPM to 100 17 Press ON OFF key to switch on parameter RPM 18 Press option key to finish timer settings Cycle symbol lights up blue Current active phase blinks blue Incubation shaker starts with settings of phase 1 and alternates with the set cycle ...

Page 177: ...Multitron Pro Incubation Shaker Appendix 2013 02 19 Page 177 of 185 12 Appendix 12 1 Declaration of Conformity ...

Page 178: ...Multitron Pro Incubation Shaker Appendix Page 178 of 185 2013 02 19 12 2 Circuit diagram Fig 110 ...

Page 179: ...n of the system with sterile filter is identical as described before The supplier s specification of 5 L of liquid volume is valid for processing of particle laden biological liquids If used for distilled water that is practically particle free the life time of the filter is much higher depending on the quality of the water It is not possible however to specify the lifetime exactly please refer to...

Page 180: ... or de ionized water is water that has had its mineral ions removed such as cations from sodium calcium iron copper and anions such as chloride and bromide Deionization is a physical process which uses specially manufactured ion exchange resins which bind to and filter out the mineral salts from water Because the majority of water impurities are dissolved salts deionization produces a high purity ...

Page 181: ...irection of the water flow due to application of pressure on one side of the membrane The rejection rate of contaminants salts from the water is generally in the region of 90 to 99 5 depending upon the type of membrane used and water to be treated This means that a typical mains water supply of around 500 to 700 µS cm conductivity when passed through a Reverse Osmosis plant will give water of a co...

Page 182: ...Multitron Pro Incubation Shaker Appendix Page 182 of 185 2013 02 19 12 5 Roxtec EzEntry 4 Mini ...

Page 183: ...Multitron Pro Incubation Shaker Appendix 2013 02 19 Page 183 of 185 ...

Page 184: ...assing station 20 Drawing with side and top cooling 20 Illumination algae 23 Illumination daylight photosynthesis 23 Incubation chamber 21 ShakerBag Option gassing 22 Disassembly Personnel 167 Safety 167 Disposal 169 E Emissions 47 Environmental protection 18 Error Acoustic 56 Visual 56 Examples Cyclic operation 174 Delay a speed change 170 Switch on delay 172 F Function Basic Functions 54 Message...

Page 185: ...ing illumination 133 Operating conditions 46 Operating panel Displays 64 Keys 65 Symbols 62 Operation Alarm 122 Cooling 132 Direct Steam Humidification 131 Ending a work cycle 120 General 105 Interrupting operation 130 Loading the StickyStuff adhesive matting 129 Loading tray Sticky Stuff 129 Operating CO2 gassing 135 Operating the mobile Pt100 sensor 137 Personnel 103 Safety 103 Selecting a unit ...

Page 186: ... 66 Direct Steam Humidification 68 Illumination 72 Operating panel 60 Option Mobile Pt100 sensor 76 Pass through 75 Reduction Station for CO2 gassing 72 Serial interface 57 Sticky Stuff adhesive matting 68 Transport lock 53 UV sterilisation 79 Signs 19 Spare parts 10 Specification Adhesive matting Sticky Stuff 38 CO2 curtain 44 CO2 gassing 43 CO2 pressure reducing unit 44 Direct steam humidificati...

Page 187: ...ning runtime 114 Single changeover 110 Transport Fork lift 82 Inspection 80 Safety 80 Trouble shooting 157 U Unauthorised persons 13 Utilities 47 W Weights 23 Base frame 24 Base unit 23 Cooling units 25 Direct steam humidification 25 Gasing ShakerBag Option 25 Gassing unit CO2 25 Illumination 25 M tray 24 Perforated tray 24 ShakerBag Tray 25 ...

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