ibidi Pump System
6.6.3
Flow Calibration in the Software
Now use the measured flow rate to update the PumpControl software in the “Recalibration factor”
menu.
1. Click on the “Recalibration factor” button to open the dialog (Figure
2. Insert the given flow rate and the one that was measured of yourself in the recalibration dialog
(Figure
). Then press “OK”.
The software will now compensate the difference in measured and expected flow rate by means of a
multiplicative calibration factor.
Example experiment:
Given flow rate
23.8 ml/min
Measured flow rate
21.05 ml/min
Resulting calibration factor
1.131
Figure 35: Recalibration dialog in the PumpControl software.
The recalibration factor affects the relationship between pressure and resulting flow rate (Figure
Figure 36: Influence of the calibration factor and viscosity on the parameters of the PumpControl
software.
Handling Tip
If you are re-using the Perfusion Sets (e.g., after autoclaving the tubings and adapters) note the
calibration factor for the next experiment. When starting a new experiment you can immediately
enter the calibration factor into the corresponding box.
For special setups, e.g., a custom slide, several slides connected serially or slides which are not im-
plemented in the software, the calibration is mandatory (Section
Instruction Manual
47
Version 2.6 (May 12, 2022)
