ibidi Pump System
3.6
Slide and Perfusion Set Selection Guide
To set up a successful experiment, select a suitable Perfusion Set and µ-Slide for the specific appli-
cation. In addition to shear stress, consider parameters such as working volume, dead volume, and
tubing length.
For the first experiment in a demo run, a red Perfusion Set (#10962) with an inner diameter of 1.6 mm
and a µ-Slide I
0.6
Luer are recommended.
Please find a detailed overview of the minimal and maximal flow rates and shear stresses that can be
applied with the ibidi Pump System in the following tables. The minimum and maximum values are
based on the minimal working pressure of 5 mbar, and the maximal working pressure of 95 mbar.
Perfusion Set Red
Viscosity: 0.0072 dyn
·
s/cm
2
Flow rate
Shear stress
Temperature: 37°C
Min.
Max.
Min.
Max.
Without any slide
4.7 ml/min
52.0 ml/min
–
–
µ-Slide I
0.2
Luer
2.0 ml/min
27.0 ml/min
7.5 dyn/cm
2
95.0 dyn/cm
2
µ-Slide I
0.2
Luer Glass
3.1 ml/min
36.0 ml/min
7.5 dyn/cm
2
82.0 dyn/cm
2
µ-Slide I
0.4
Luer
4.2 ml/min
47.0 ml/min
4.0 dyn/cm
2
43.0 dyn/cm
2
µ-Slide I
0.4
Luer Glass
4.6 ml/min
52.0 ml/min
3.5 dyn/cm
2
38.0 dyn/cm
2
µ-Slide I
0.6
Luer
4.5 ml/min
49.0 ml/min
2.0 dyn/cm
2
20.0 dyn/cm
2
µ-Slide I
0.6
Luer Glass
5.2 ml/min
51.0 ml/min
1.5 dyn/cm
2
18.0 dyn/cm
2
µ-Slide I
0.8
Luer
4.5 ml/min
50.0 ml/min
1.5 dyn/cm
2
12.0 dyn/cm
2
µ-Slide I
0.8
Luer Glass
4.1 ml/min
53.0 ml/min
1.0 dyn/cm
2
12.0 dyn/cm
2
µ-Slide VI
0.4
4.1 ml/min
47.0 ml/min
5.0 dyn/cm
2
58.0 dyn/cm
2
µ-Slide VI
0.5
Glass
4.6 ml/min
52.0 ml/min
3.5 dyn/cm
2
35.0 dyn/cm
2
µ-Slide VI
0.1
0.3 ml/min
3.3 ml/min
19.0 dyn/cm
2
245.0 dyn/cm
2
µ-Slide I Luer 3D
4.3 ml/min
52.0 ml/min
2.0 dyn/cm
2
22.0 dyn/cm
2
µ-Slide Spheroid Perfu-
sion
0.3 ml/min
2.5 ml/min
–
–
µ-Slide y-shaped*
3.8 ml/min
42.0 ml/min
6.0 dyn/cm
2
67.0 dyn/cm
2
*The indicated values for the µ-Slide y-shaped refer to the single channel area (see
Instruction Manual
25
Version 2.6 (May 12, 2022)
