Instructions
Glass Bottom Dish
35 mm
Seeding Cells
Depending on your cell type, application of a 4–9 ×
10
4
cells/ml suspension should result in a confluent layer
within 2–3 days.
• Trypsinize and count cells as usual. Dilute the cell
suspension to the desired concentration.
• Apply 400 µl cell suspension into the inner well of
the µ–Dish. Avoid shaking as this will result in inho-
mogeneous distribution of the cells.
• After cell attachment add additionally 1.6 ml of pure
medium to ensure optimal grow conditions.
• Cover the µ–Dish with the supplied lid. Incubate at
37°C and 5 % CO
2
as usual.
We recommend not to fill more than the indicated to-
tal volume into the Glass Bottom Dish
35 mm
in order to
avoid the liquid contacting the lid.
Undemanding cells can be left in their seeding medium for
several days and grow to confluence there. However, best
results are achieved when the medium is changed every
2–3 days. Carefully aspirate the old medium and replace
it by up to 2 ml fresh medium.
Glass Bottom Dish Selection Guide
Glass Bottom
Dish
35 mm
µ–Dish
35mm, high
Glass Bottom
Lid
normal
with Lid Lock po-
sition
Evaporation
medium
low
Coverslip
No. 1.5
No. 1.5H
Thickness
170 µm
(+20 µm/-10 µm)
170 µm (±5 µm)
Packaging
10 pieces
1 piece
Note:
If cultivated in a dry atmosphere, evaporation can
harm the cells during longer experiments. Exchange
the medium every 1-2 days to minimize evaporation-
mediated osmolality shifts.
Optional Glass Coverslip Cleaning Protocol
The Glass Bottom Dish
35 mm
is made with an uncoated
glass coverslip. For improved cell attachment, glass sur-
face modifications and other applications, the glass cov-
erslip of the Glass Bottom Dish
35 mm
can be cleaned by
following the protocol below.
• Remove lids and immerse products in ddH
2
O in an
appropriately sized beaker.
• Sonicate for 10 minutes.
• Decant the ddH
2
O completely.
• Add 1 M HCl.
• Sonicate for 10 minutes.
• Decant the HCl completely and wash twice with
ddH
2
O. Decant the ddH
2
O completely.
• Add 2-propanol (absolute).
• Sonicate for 10 minutes.
• Aspirate the 2-propanol completely. Make sure that
all products are completely dry. Wash twice with
ddH
2
O and aspirate the ddH
2
O completely.
• Add ethanol (absolute).
• Sonicate for 10 minutes.
• Aspirate the ethanol completely.
Make sure that
all products are completely dry. Wash twice with
ddH
2
O.
• Sonicate in ddH
2
O for 10 min.
• Decant ddH
2
O and blow dry carefully with canned
air or clean nitrogen gas.
Modifications of this protocol including acids, bases, alco-
hols and detergents are possible. Please check the chem-
ical compatibility list on
for compatibil-
ity. Make sure to handle the glass-bottomed products with
care. The glass coverslips may break during mechani-
cal handling. For best results, use a custom-made Teflon
holder.
Glass Bottom Dish
35 mm
Page 2
Version 1.1 (2018-07-11)
