ibidi Gas Incubation System – Silver Line
4.4
Heated Tubing Package
The Heated Tubing Package connects the gas output of the Gas Mixer to the Humidifying Column
and to the Incubation Chamber on the microscope stage (Figure
). To actively control the humidity,
two gas outputs are needed. One leads to the Humidifying Column and the other bypasses it. Both
streams are combined in the tubing leading to the Incubation Chamber. By varying the flow rates of
the two gas streams, relative to each other, the humidity is actively controlled (Figure
The gas tubing carrying humidified gas is heated up to avoid condensation. For this purpose, a
heating wire is inserted in the tubing. The wire is split off of the electrical cable under the insulating
jacket enclosing the tubing. The exact connection of the tubing is shown in Figure
A
B
C
D
E
F
G
H
I
1
2
Humidifying
Column
Incubation
Chamber
Gas Mixer
J
Figure 7: Heated Tubing Package: Electrical cable for the heating of the Humidifying Column (A),
insulating jacket (B), Mixed gas output white and yellow (C and D), heated wire split off the electrical
cable (E), gas input to Humidifying Column (F), gas output from Humidifying Column (G), merging
point, where the heated wire is led into the tubing (H), gas tubing to the Incubation Chamber (I), gas
tubing to push-in fittings of the Incubation Chamber Slide/Dish – Silver Line (J).
CAUTION
– Do not cut the tubing leading to the incubation chamber! This would dam-
age the heated wire in the inside and lead to malfunction.
Instruction Manual
21
Version 1.0 (June 1, 2022)
