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HRM-300 Upright Microscope

10. Specifications

Body unit

Item

HRM-300 (TRF, TRF-P)

HRM-300M (RF, RF-P)

1.Optical System

Infinity-corrected
optical system

2.Illumination

Reflected / Transmitted

Reflected

3.Base unit

Focus

Stroke range: 29.5 mm
Coarse and Fine focusing knob.
Coarse: 17.7 mm/revolution, Fine: 0.1 mm/revolution (1㎛ scale)
Upper limit stopper, Torque adjustment ring

Electronics

Ratings: 100V-240Vac. 1.2/2.2A, 50/60Hz
Fuse: 250Vac. T4L
Integrated power supply for DC 12V100W light adjustment.
LED voltage indicator, IL switch and ECO switch
Power consumption: 150W

4.Transmitted illumination

Kohler illumination system
Brightfield
Built-in filters(ND6, ND25, LB)

Light Source

HM-LH100: 12V100W halogen

Lamp

12V 100W long-life halogen bulb
(Philips 7724)

5.Condenser

(Not suitable for Phase Contrast)

Type

Abbe

Long WD

N.A

1.20

0.65

6.Stage

4x4 inch

HR-ST4R: metal plate, glass plate
Size 210(W) x 170(D) mm, Stroke 104(X) x 102(Y) mm

3x2 inch

HR-ST3R: glass plate, specimen holders
Size 180(W) x 160(D) mm, Stroke 79(X) x 55(Y) mm

6x4 inch

HR-ST6X4R: metal plate, glass plate
Size 299(W) x 193(D) mm, Stroke 154(X) x 104(Y) mm

6x6 inch

HR-ST6R: metal plate, glass plate
Size 240(W) x 260(D) mm, Stroke 153(X) x 153(Y) mm

7.Observation
Tube

Trinocular

HM-WETR: Erect type(F.N 25), Incline angle 20˚,

Φ30

sleeve

Light path selection: 2-step (Eye : Camera

–

100 : 0 , 0 : 100)

Binocular

HM-WEBI: Erect type(F.N 25), Incline angle 20˚, Φ30 sleeve

8.Eyepiece

HM-EPA10x: 10x(F.N 25), Diopter adjustable, Φ30 sleeve

Reticle

Crosshair, X-micrometer, XY-micrometer, Grid

9.Revolving
nosepiece

BF/DF

HM-D5BDRE: 5-position Nosepiece for Dark-field objective lens with
DIC slot

BF

HM-D5RE: 5-position Nosepiece for Bright-field objective lens with
DIC slot

10.Dimension

Approx. 246(W) x 572(D) x 514(H)

11. Weight

Approx. 19kg

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Summary of Contents for HRM-300

Page 1: ...1 HRM 300 Upright Microscope 표지그림 변경 부탁합니다 ...

Page 2: ...e of publication HUVITZ assumes no responsibility however for possible errors or omissions or for any consequences resulting from the use of the information contained herein HUVITZ reserves the right to make changes in its products or product specifications at any time and without prior notice and is not required to update this documentation to reflect such changes 9000ENGN001 A 2012 HUVITZ Co Ltd...

Page 3: ...clature 13 7 Observation Procedure Transmitted BF TRF TRF P 16 7 1 Preparation 16 7 2 Procedure 16 8 Operation 18 8 1 Base 18 8 1 1 Voltage Indication and Switch 18 8 1 2 Turn ON OFF Power saving function 18 8 1 3 Turn ON OFF Intelligent light function 19 8 1 4 Using the built in filters TRF TRF P 19 8 2 Focusing Block 20 8 2 1 Replacing the Fine adjustment knob 20 8 2 2 Adjusting the Coarse adjus...

Page 4: ... 8 6 2 Height display 28 8 6 3 Safety function 29 8 7 Linking Panasis RF TRF 29 8 7 1 Computer control 29 9 Troubleshooting Guide 30 10 Specifications 32 11 Assembly 34 11 1 Assembly Diagram 34 11 2 Detailed Assembly Procedure 35 11 2 1 Installing the Bulb TRF TRF P 35 11 2 2 Attaching the Lamp housing TRF TRF P 36 11 2 3 Attaching the Condenser 37 11 2 4 Attaching the Stage 37 11 2 5 Attaching th...

Page 5: ...rator It is for this reason that the following safety notices have been placed appropriately within the text of this manual to highlight safety related information or information requiring special emphasis All users operators and maintainers must be familiar with and pay particular attention to all Warnings and Cautions incorporated herein Warning indicates the presence of a hazard that could resu...

Page 6: ... ground is fastened to the chassis Protective earth connected to conductive parts of Class I equipment for safety purposes This symbol identifies Alternating Current Disposal of your old appliance When this crossed out wheeled bin symbol is attached to a product it means the product is covered by the European Directive 2002 96 EC All electrical and electronic products should be disposed of separat...

Page 7: ... equipment Where the humidity is extremely high or there is a ventilation problem Where the equipment is subject to excessive shocks or vibrations Where equipment is exposed to chemical material or explosive gas Be careful not to be inserted dust especially metal Don t disassemble the product or open We aren t responsible for it for nothing Don t plug the AC power cord into the outlet before the c...

Page 8: ...lcohol benzene or similar chemicals 2 Do not put or use this device in humid rooms Humidity should be maintained between 30 and 85 for normal operation Do not expose the device to water splashes dripping water or sprayed water Do not place containers containing fluids liquids or gases on top of any electrical equipment or devices 3 The equipment must be operated only by or under direct supervision...

Page 9: ...n used in an observation of a specimen that is accompanied with a potential of infection clean the parts coming in contact with the specimen to prevent infection 15 ln case the specimen is damaged by erroneous operation promptly take the infection prevention measures 16 Power Supply Cord set international Detachable Rated 10 A 250 V Plug type CEE 7 VII Connector type IEC 60320 C13 and Cord type H0...

Page 10: ...asonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in whi...

Page 11: ...precision instrument Handle it with care and avoid subjecting it to sudden or severe impact When moving the microscope first remove the specimens eyepieces and filters so that they will not drop Also remove other modules e g lamp housing stage for decreasing system weight Then securely grasp the microscope by the rear of arms and the base Do not grasp focus adjustment knob binocular tube lamp hous...

Page 12: ...nents because they use plastic resin materials extensively To clean them use a lint free soft cloth slightly moistened with a diluted neutral detergent Do not disassemble any part of the microscope as this could result in malfunction or reduced performance When disposing of the microscope check the regulations and rules of your local government and be sure to observe them Since the absolute alcoho...

Page 13: ...adjustment knob TRF TRF P 26 page Filter switch TRF TRF P 19 page Focus tension adjustment ring 21 page Field iris diaphragm ring TRF TRF P 26 page XY stage knob ECO button 18 page Voltage indicator 18 page Intelligent light Button 19 page Light path selector knob ...

Page 14: ...roscope Interpupillary distance adjustment scale 24 page Pre focusing Lever 22page Brightness adjustment knob 18 page Diopter adjustment ring 24 page Control box connector RF P TRF P 39 page Power switch Panasis port 39 page ...

Page 15: ...Main body connector 39 page Fine focus adjustment knob 28 page Emergency stop 29 page Control pad connector 39 page Power switch Focus adjustment button 28 page Panasis Port 39 page Display window 28 page Brightness adjustment knob 28 page Origin display button 28 page Reset button 28 page ...

Page 16: ...e specimen Coarse fine adjustment knob 7 Adjust brightness Brightness control knob page 18 8 Adjust diopter if light path selection is eye Diopter adjustment ring page 24 9 Adjust interpupillary distance if light path selection is eye Binocular tube page 24 10 Adjust the height and position of condenser Condenser height adjustment knob page 26 Condenser centering knob page 26 11 Engage the desired...

Page 17: ...17 ...

Page 18: ...se to increase the voltage and make illumination brighter and vice versa The indicator indicate the voltage 8 1 2 Turn ON OFF Power saving function Push ECO button to toggle power saving function on and off The ECO indicator LED turns on when the power saving function turns on If the power saving function turns on lamp is automatically turns off after 5 minute when there is nobody sitting in front...

Page 19: ...e revolving nosepiece is changed To set new preset brightness press IL button long while IL indicator LED turns on 8 1 4 Using the built in Filters TRF TRF P Each of filter switch can engaged into the light path by push up the switch Push down the switch disengages the filter from light path Filter Type Purpose ND6 Neutral Density Filter for light adjustment transmittance 6 ND25 Neutral Density Fi...

Page 20: ...e adjustment knob is designed detachable to prevent interference with hand during manipulation of the fine adjustment knob or XY axis knob Usually attach the knob on the opposite side to the XY axis knob Using the Allen screwdriver loosen the clamping screw and remove the fine adjustment knob Fig 5 ...

Page 21: ...ustment tension using the tension adjustment ring Turing the ring clockwise increase tension and vice versa If the tension is too low the stage drops by itself and focus is quickly lost after adjustment with the fine adjustment knob In this case turn the ring clockwise to increase tension Fig 6 ...

Page 22: ...ent is set at the locked position After changing a specimen refocusing is easily accomplished by rotating the coarse adjustment knob to reach the pre focused position then making fine adjustment with the fine adjustment knob Turn the lever counterclockwise to unlock When the pre focusing lever is locked a coarse movement stroke is reduced due to the microscope s mechanism causing the stage not to ...

Page 23: ...23 8 3 Stage 8 3 1 Rotating the Stage Slightly loosen the stage clamping screw The stage can be rotated both direction by the stage clamping screw Fig 8 ...

Page 24: ... the interpupillary distance 8 4 2 Adjusting the Diopter The diopter adjustment ring is used when the eyepiece has no function of diopter adjustment Looking through the right eyepiece there is no diopter adjustment ring rotate the coarse and fine adjustment knobs to bring the specimen into focus Looking through the left eyepiece there is a diopter adjustment ring turn only the diopter adjustment r...

Page 25: ...lasses extend the folded eye shades in direction of the arrow to prevent extraneous light from entering between the eyepieces and eyes 8 4 4 Selecting the Light Path of the Trinocular Tube Slide the light path selector knob to select the desired light path Light Path Selector Knob Indication Light Path Ratio Pushed in position Binocular 100 Pulled out position TV Photo 100 Fig 11 Fig 12 ...

Page 26: ... 5 Turn the two condenser centering knob to move the iris diaphragm image to the center of the field of view 6 Gradually open the field iris diaphragm The condenser is properly centered if the iris image is centered and inscribed in the field of view 7 During actual use open the field diaphragm slightly until its image circumscribes the field of view Fig 14 The field iris diaphragm restricts the d...

Page 27: ...ecimens is ordinarily low setting the condenser aperture iris diaphragm to between 70 and 80 of the NA of the objectives in use is usually recommended Remove one of the eyepieces look into the eyepiece sleeve and adjust the aperture iris diaphragm ring until the image is about 70 of objective s exit pupil You can use the condenser numerical aperture scale to adjust the aperture iris diaphragm Fig ...

Page 28: ...nge precision of control Rotate the fine focus adjustment knob to change voltage of lamp 8 6 2 Height display Height of 3D profiler is displayed in height display window Two type of height can be displayed a Absolute height height from 3D profiler s mechanical origin b Relative height height from origin where user set Push origin display button to toggle absolute height relative height Push reset ...

Page 29: ... emergency stop button to stop 3D profiler immediately Use this button when the objectives is about to contact with the specimen 8 7 Linking Panasis RF TRF 8 7 1 Computer Cotrol Some parameter can be controlled by using Panasis program Run Panasis program in computer Select Microscope Huvitz Upright in menubar Parameter of IL and ECO function can be changed in microscope control window For the pre...

Page 30: ...dark Lamp voltage is too low Adjust brightness with brightness control knob 18 Light path selection knob is set to 100 camera position Set to 100 eyepiece position 25 Aperture and field diaphragm is stopped down too far Open to a suitable size 27 Condenser is not centered properly Center the condenser 26 Nosepiece is not rotated to a clickstop position Rotate the nosepiece to a click stop position...

Page 31: ...ust exists on the specimen C Observation Tube 1 Field of view does not match with both eyes Interpupillary distance is not correct Adjust the interpupillary distance 24 Eyepiece diopter is not adjusted Adjust the diopter 24 Different eyepiece is used on left and right Use the same type magnification field number on both sides D Stage and Focus Adjustment 1 Coarse adjustment knob is hard to turn Te...

Page 32: ...or Phase Contrast Type Abbe Long WD N A 1 20 0 65 6 Stage 4x4 inch HR ST4R metal plate glass plate Size 210 W x 170 D mm Stroke 104 X x 102 Y mm 3x2 inch HR ST3R glass plate specimen holders Size 180 W x 160 D mm Stroke 79 X x 55 Y mm 6x4 inch HR ST6X4R metal plate glass plate Size 299 W x 193 D mm Stroke 154 X x 104 Y mm 6x6 inch HR ST6R metal plate glass plate Size 240 W x 260 D mm Stroke 153 X ...

Page 33: ...r objectives PlanFluor objectives is not suitable for phase contrast and has no iris diaphragm or correction collar Objective Lens Magnifi cation N A W D mm Cover glass Thickness mm Field of view mm Eyepiece HM EPA10x PlanFluor EPI 5x 0 15 20 5 10x 0 30 11 2 5 20x 0 45 3 0 1 25 50x 0 80 1 0 0 5 100x 0 90 1 0 0 25 PlanFluor EPI BD 5x 0 15 20 5 10x 0 30 11 2 5 20x 0 45 3 0 1 25 50x 0 80 1 0 0 5 100x...

Page 34: ...r touching glass surfaces Most assembly operations are possible by using the Allen screwdriver provided with the microscope Observation tube HM WETR HM WEBI Body HR3 RF HR3 RF P HR3 TRF HR3 TRF P Condenser HR ACD HR LWCD Revolving nosepiece HM D5BDRE HM D5RE Stage HR SR4R HR ST3R HR ST6x4R HR ST6R Eyepiece HM WEPA10x Camera mount HM TV1xC HM TV0 5xC HM TV0 35xC Reflected light illuminator HR3 RLI ...

Page 35: ...using base from the above Tighten the clamping screw while pressing downward on the cover Fig 21 Fig 22 The bulb the lamp socket and areas near these will be extremely hot during and right after use Set the main switch off disconnect the power cord from the wall outlet then wait until lamp housing has cooled down before replacing the bulb with a new of the designated type To prevent reduced bulb l...

Page 36: ...ned the clamping screw with provided Allen screwdriver 4 Plug the cable coming from the lamp housing into the lamp connector on the rear of the microscope and tighten the ring of the connector to secure the connection Fig 23 The bulb the lamp socket and areas near these will be extremely hot during and right after use Set the main switch off disconnect the power cord from the wall outlet then wait...

Page 37: ...position 3 Fully loosen the condenser clamping knob 4 Position the condenser so that the aperture scale is located in front 5 Tighten the condenser clamping knob and gently raise the condenser holder to its height position 11 2 4 Attaching the Stage 1 Turn the coarse adjustment knob to lower the stage to its lower limit 2 Place the stage on the substage and tighten the stage clamping knob Fig 24 F...

Page 38: ...top of illuminator and tighten the observation tube clamp screw 11 2 6 Attaching the Eyepiece 1 Insert eyepiece to the eyepiece sleeve and press the eyepiece as far as it goes Attach eyepieces of the same magnification and of the same field number to the left and the right eyes Otherwise it is hard to observe specimens because of the difference of left and right images Fig 26 Fig 27 ...

Page 39: ...12pin cable and fasten it 3 Connect control pad connecter with provided 6pin cable and fasten it 4 Connect control box connector with another end of 12pin cable and fasten it 5 Connect Panasis port and computer with RS232C cable when using Panasis program 11 2 8 Attaching the Computer RF TRF 1 Connect Panasis port and computer with RS232C cable when using Panasis program Fig 28 Fig 29 ...

Page 40: ...e following information Name of the product HRM 300 Upright microscope Serial number of the product refer to the 9 digit number on its product label or name plate Descriptions of Problem In detail Date of Purchase Dealer s Name Dealer Address Dealer Phone No Version No Serial No Huvitz recommends customers to fill up the above form after purchase and retain this manual as a permanent record of pur...

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