4
alcohol and 500ml petroleum ether into the A container;
②
Take 1 bottle of extract medicine block (see above) into reaction cup B, add 10ml
isopropanol and 10ml petroleum ether, stir the extract medicine block, isopropanol and
petroleum ether in the reaction cup, stir and dissolve into the pre In the prepared A
container;
③
Pour 10ml of petroleum ether and 10ml of isopropanol into the B reaction cup. After
mixing petroleum ether, isopropanol and the residue, pour it into the A container again and
stir evenly;
④
Finally, the mixed liquid in container A is poured into empty glass bottles (empty bottles
containing isopropyl alcohol and petroleum ether can be used), the cap is screwed tightly
and shaken sufficiently to completely dissolve, and the extraction liquid is completed.
（
3
）
Extraction
：
The essential materials include a container(a clean and dry drinking water
bottle is allowed) that more than 1000ml capacity, a reaction cup,500ml Isopropyl Alcohol
and 500ml Petroleum Ether. Open the plastic bag with the extract provided with the
instrument, put the extract in the reaction cup, and add a small amount of isopropyl
alcohol and petroleum ether to dissolve, transfer the solution to the large container
prepared in advance, then wash the reaction cup with petroleum ether and isopropyl
alcohol several times. The cleaning liquid is poured into the large container together. The
remaining petroleum ether and isopropyl alcohol are all poured into the large container
and shaken. At last, inject the solution in the blank bottles which contained Isopropyl
Alcohol and Petroleum Ether.
（
4
）
Standard acid solution
：
The solution is the 0.15mol/l Potassium hydrogen phthalate
solution
，
which can access directly in the small glass bottle.
（
5
）
BTB aqueous solution
：
Open the bag with BTB provided with the instrument and
transfers it in the 100ml container as soon as possible. Then inject 100ml Anhydrous
Ethanol and shake it completely. Draw 0.5 ml of this solution with the graduated pipette
and add 50ml distilled water, and then there is the BTB aqueous solution.
（
6
）
Washing solution
：
Transfer the white solid in the glass bottle labeled with KOH to the
wash gas cylinders as soon as possible. Then inject 50ml water and shake it
completely .At last set aside to use.
