Temperature [°C]
Pressure [kPa]
95.0
101.3
105.0
20
1.0028
1.0029
1.0029
21
1.0030
1.0031
1.0031
22
1.0032
1.0033
1.0033
23
1.0034
1.0035
1.0036
24
1.0037
1.0038
1.0038
25
1.0039
1.0040
1.0040
Model
Range
of the pipette
volumes
[μl]
Preset
volume
[μl]
Permissible
volumes
[μl]
Volume
change
D
V
for full turn
of the
calibration key
[μl]
(24 increments)
DV2, D2
0.1 - 2
0.2
0.176 - 0.224
0.06
DV10, D10
0.5 - 10
0.5
0.48 - 0.52
0.33
DV20, D20
2 - 20
2
1.94 - 2.06
0.63
DV50, D50
5 - 50
5
4.875 - 5.125
2.50
DV100, D100
10 - 100
10
9.84 - 10.16
2.50
DV200, D200
20 - 200
20
19.76 - 20.24
6.30
DV250, D250
50 - 250
50
49.5 - 50.5
6.30
DV1000, D1000
100 - 1000
100
98.4 - 101.6
25.00
DV5000, D5000
500 - 5000
500
494 - 506
125.00
DV10000, D10000
1000 - 10000
1000
975 - 1025
250.00
ENGLISH
• Turn the key clockwise to reduce the aspirated volume,
or counter-clockwise to increase the volume, (Fig. 4C).
One full turn of the calibration key changes the pipette
aspiration volume by the amount given in the table.
• Take out the key and fix the pipetting pushbutton (Fig. 4D).
The pipetting pushbutton should be fixed by placing the
knob on the arbor first (Fig. 1A2) and then the pushbutton
(Fig. 1A1).
Determine the average aspirated volume. The average
volume should be within the permissible range given in
the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties consi de -
rably different from those of water, follow the rules given
in section 5.
More information on calibration procedure can be found
on
www.htl.com.pl
9 - TROUBLESHOOTING
If you notice an improper pipette operation identify the
cause and eliminate the fault. To do this, follow the
instruction in the sequence provided. Replacement of
parts should be required only occasionally, and should
not occur under normal pipette use.
Droplets of liquid remain in the pipette tip.
• The tip is emptied too fast.
Decrease the speed of pressing the pipette push-
button.
• The tip wettability has increased due to extensive use.
Replace the tip with a new one.
Droplets of air appear in the liquid aspirated into the tip.
• The pipette tip immersion is too shallow.
Immerse the tip deeper according to the instructions.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
Press the pipette firmly.
• The tip is damaged or worn out due to extensive use.
Replace the tip with a new one.
The pipette incorrectly aspirates the liquid or liquid
drops out from the tip.
• The pipette tip is incorrectly pressed onto the pipette
shaft.
12
11
• Perform 5 aspirations, weigh each one and calculate
the average value of the aspirations
• Calculate average aspirated volume in μl multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μl/mg], which depends on
temperature and pressure according to the following
table:
If the average aspirated volume exceeds the per missible
value, the following should be done:
• Remove the pipetting pushbutton, (Fig. 4A),
Warning: The pipetting pushbutton consists of 2 parts:
the knob (Fig. 1A2) and the pushbutton (Fig. 1A1). After
removal of the pushbutton, both parts are separated.
• Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw, (Fig. 4B),
