horiba ABX MICROS ES60, User Manual

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Description & Technology
User Manual - RAB237AEN 3–5
3. Technology
3.1. Sampling
3.1.1. ABX Micros ES60 CT
1- Blood collection tube is placed into the tube holder.
2- The tube holder door is then closed by the operator.
3- The cap-piercing mechanism moves the tube holder up and pierces the sample tube.
4- Aspiration of 10µl of blood.
5- Needle carriage assembly moves to the left, over the WBC/HGB chamber.
6- Internal and external cap-piercing needle rinses. External sampling needle rinses.
7- Blood sample is delivered into the WBC chamber for the first dilution.
8- Aspiration of 28.3µl of diluted blood from the WBC chamber.
9- Needle carriage assembly moves to the right, over the RBC/PLT chamber.
10- Internal and external cap-piercing needle rinses. External sampling needle rinses.
11- Diluted blood sample is delivered into the RBC chamber for the RBC/PLT measurement.
3.1.2. ABX Micros ES60 OT
1- Blood collection tube cap must be removed.
2- The tube is placed beneath the sampling needle.
3- The tube is moved upwards and the sampling needle enters into the blood.
4- The manual sample bar is pressed and aspiration of 10µl of blood occurs.
5- Needle carriage assembly moves to the left, over the WBC/HGB chamber.
6- The external sampling needle rinses.
7- Blood sample is delivered into the WBC chamber for the first dilution.
8- Aspiration of 28.3µl of diluted blood from the WBC chamber.
9- Needle carriage assembly moves to the right, over the RBC/PLT chamber.
10- The external sampling needle rinses.
11- Diluted blood sample is delivered into the RBC chamber for the RBC/PLT measurement.
3.2. Dilutions
3.2.1. WBC/HGB
Tab.3-1 WBC/HGB dilution
Tab.3-2 WBC measurement
WBC/HGB dilution
Initial blood volume 10.0µl
Volume of ABX Minidil LMG 2.1ml
Volume of ABX Lyse 0.5ml
Final dilution ratio 1/260
WBC measurement
Method Impedance
Aperture diameter 80µm
Count vacuum 200mb
Count period 2 (or 3) x 6 seconds
Temperature reaction Ambient