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 Home Training Tools Ltd. 2012                                        Page 5 of 8                                         Visit www.homesciencetools.com 

 

100x Objective 

Begin looking at any specimen with the iris 

diaphragm all the way open (clockwise is open, 
counter-clockwise is closed). After focusing, 
reduce the light by moving the lower lever until the 
specimen is in sharp, clear contrast. As with the 
disc diaphragm, you will need less light on lower 
power and more light on higher power. The 
following table gives suggested opening sizes for 
each power level:  

 

Objective Diaphragm 

Opening 

4x 

From fully closed to 1/8 open

10x 

1/8 to 1/4 open  

40x 

1/4 to 1/2 open  

100x 

1/2 to 3/4 open  

 
Mechanical Stage 

The MI-4100LMS, 4100LDH, 4100LXL, and 

4100LBH models have a mechanical stage built-in, 
a component that allows precise movement of the 
slide on the stage while maintaining relatively good 
focus. It is ideal for scanning specimens. The 
mechanical stage has three operating components: 
the slide clamp arm, the “x” or right/left adjustment 
knob and the “y” or front/back adjustment knob.  

1.

 

Move the small lever on top of the stage to 
open the curved slide clamp arm.  

2.

 

Carefully place the slide squarely against the 
fixed side and back edge of the mechanical 
stage. Make sure the slide lies flat on the 
microscope stage.  

3.

 

Gently release the small lever allowing the 
slide clamp arm to securely hold the specimen 
slide in place. Releasing the lever too quickly 
may damage slides. 

4.

 

Move the specimen under the objective lens by 
turning the adjustment knobs. The front/back 
adjustment knob is at the top. It allows precise 
movement to the front and back of the stage. 
The right/left adjustment knob is at the bottom, 
towards the base of the microscope. It allows 
precise movement to the right and left of the 
stage.  

5.

 

Turn these adjustment knobs without putting 
any pressure on the stage. This will allow you 
to scan a slide while maintaining fairly good 
focus. The integrated mechanical stage has a 
70 mm range of motion along the “y” axis and 
a 30 mm range of motion along the “x” axis. 

Filters 

The MI-4100LXL and 4100LBH models come 

with a blue filter that can be placed in the filter 
holder located below the iris diaphragm. The filter 
absorbs some of the light from the illuminator and 
makes the light slightly blue. In some cases this 

may enhance the image of 
your specimen, though 
generally it is not necessary. 
Use the following procedure 
to insert the filter:  

1.

 

Make sure the Abbe 
condenser is all the way up. 

2.

 

Locate the tiny filter holder knob underneath 
the iris diaphragm. Use this to swing the filter 
holder out from under the diaphragm.  

3.

 

Place the filter in the holder and swing back 
into place.  

4.

 

Adjust focus and diaphragm normally.  

 

100x Oil Immersion Objective  

The MI-4100LXL and 4100LBH model has an 

additional 100x objective that provides 1000x 
magnification. At this magnification it is important 
to reduce light diffraction to enhance the image 
quality. This is done by using immersion oil 
according to the following steps:    

1.

 

Lower the stage using the 
coarse focus knob.  

2.

 

Raise the Abbe Condenser 
to the highest position by 
turning its lever counter-
clockwise. Open the iris 
diaphragm by turning its 
lever clockwise.  

3.

 

Place one drop of high quality immersion oil 
(our item MI-IMMOIL) on top of the coverslip. 
(In some cases, it may be beneficial to place a 
drop of oil between the condenser and the 
slide also, but this is usually not necessary for 
a good image.)   

4.

 

Move the 100x objective lens into position, and 
then slowly move the stage up until the lens 
makes contact with the oil.  

5.

 

Continue focusing with the coarse knob until 
the color or blurred outline of the specimen 
appears. Finish focusing with the fine focus 
knob.  

6.

 

Adjust the condenser and diaphragm until you 
see the specimen in clear, sharp contrast.  

7.

 

When you are done or ready to switch to a 
lower objective, clean the oil off the lenses and 
slides using lens paper and lens cleaning 
solution (instructions are on page 2).  

8.

 

Never leave oil on objectives or slides for 
extended periods of time, as it becomes 
extremely difficult to remove. 

Summary of Contents for MI-4100LST

Page 1: ...Home Iris Diaphragm Microscope MI 4100LDH Home Dual head Microscope MI 4100LXL Home LED 1000x Microscope MI 4100LBH Home Binocular Microscope 1 800 860 6272 www homesciencetools com Copyright 2012 by...

Page 2: ...Adjusting Tension 7 Troubleshooting 7 Specifications 8 Warranty 8 General Microscope Care Unpacking The Home microscopes are shipped in a two part Styrofoam case Keep this case for storage transport...

Page 3: ...nd down when you turn the focus knobs allowing you to control the distance between the slide and the lens The MI 4100LXL 4100LMS 4100LDH and 4100LBH models have an integrated mechanical stage that all...

Page 4: ...is for the first time If you do not have a prepared slide place a strand of colored yarn or thread on a blank slide and place a coverslip over it Move the slide until the specimen is under the objecti...

Page 5: ...m range of motion along the y axis and a 30 mm range of motion along the x axis Filters The MI 4100LXL and 4100LBH models come with a blue filter that can be placed in the filter holder located below...

Page 6: ...wobble When the head is stable the slide is easier to view 6 To readjust the binocular head loosen the screw turn the head it rotates 360 and then tighten the set screw making sure the head is stable...

Page 7: ...e to drift down under its own weight and moving the image out of focus The tension adjustment collar is located between the microscope arm and the coarse focus knob on the right side when the stage is...

Page 8: ...th slip clutch and tension adjustment Focus Rack All metal rack and pinion focusing with adjustable stage stop Stage Acid and chemical resistant 110 x 120 mm metal stage with stage clips and predrille...

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