Hologic ThinPrep 5000, Operator'S Manual, Page: 122 / 346

ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.15
5
N
ON
-G
YNECOLOGIC
S
AMPLE
P
REPARATION
MUCOID SPECIMENS
Mucoid Specimens may include respiratory and gastrointestinal specimens.
1. Collection:
Collect sample directly into 30 mL of CytoLyt
™
Solution. OR
Add 30 mL of CytoLyt Solution to the fresh specimen as soon as possible.
Note:
Large specimens (greater than 20 mL) should be concentrated
before addition of CytoLyt Solution to the sample.
Optional: If DTT is being used with respiratory mucoid samples, add stock before
agitation. See the following page for preparation instructions.
2. Mechanical agitation (page 5.11)
Note:
Vortex for a minimum of 5 minutes in “hands-free” vortexor.
3. Concentrate by centrifugation — 600g for 10 minutes (page 5.5) or 1200 g
for 5 minutes.
4. Pour off supernatant and vortext to resuspend cell pellet (page 5.7)
5. Evaluate cell pellet appearance (page 5.9)
Confirm the cell pellet is in liquid form. If the cell pellet is not in liquid
form, add 30 mL of CytoLyt Solution and repeat steps 2–4.
6. Add an appropriate amount of Specimen (dependent on the size of the
cell pellet) to PreservCyt
™
Solution vial (page 5.10)