Hologic ThinPrep 5000, Operator'S Manual, Page: 117 / 346

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YNECOLOGIC
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AMPLE
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REPARATION
5.10
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5
ADD SPECIMEN TO PRESERVCYT SOLUTION VIAL
Determine the cell pellet size and refer to the table below:
Factors to Consider
The type of pipette that you use may affect the concentration of the sample that is added to the
PreservCyt Solution vial, and therefore may affect the volume of sample. Hologic recommends using
standard, 1 mL, graduated, plastic pipettes.
If a “Sample Is Dilute” message occurs repeatedly and specimen remains in the specimen tube,
increase the number of drops of concentrated sample added to the vial.
Your technique for pouring off the supernatant may also affect the concentration of the sample. If the
supernatant is not completely poured off, then additional drops of the sample may be required. The
total volume added to the vial must not exceed 1 mL.
Size of Cell Pellet Procedure
Pellet is clearly
visible and the
pellet volume is less
than 1 mL. Place the centrifuge tube in a vortexor to resuspend
the cells in the residual liquid or mix the pellet by
syringing it manually with a pipette.
Transfer 2 drops of the pellet to a fresh PreservCyt
™
Solution vial.
Pellet is not visible
or is scant. Add the contents of a fresh PreservCyt Solution vial
(20 mL) into the tube.
Vortex briefly to mix the solution and pour the entire
sample back into the PreservCyt Solution vial.
Pellet volume is
greater than 1 mL.
Add 1 mL of CytoLyt
™
Solution into the tube. Vortex
briefly to resuspend the pellet. Transfer 1 drop of the
specimen to a fresh PreservCyt Solution vial.