1. TE70X Semi-dry Blotters:
Description
The TE70X, TE77X, TE70XP, and the TE77XP
semi-dry blotters rapidly transfer proteins from
polyacrylamide gels onto a membrane by means
of a low current and low voltage electrotransfer
with minimal Joule heating. Most transfers are
complete in one hour or less.
The smaller TE70X unit transfer surface is
14 × 16 cm, suitable for transferring standard
gels, including those from the SE600 Chroma
and the SE400. The larger TE77X transfer
surface is 21 × 26 cm, suitable for transferring
large format gels.
The TE70X and TE77X have safety circuits built
into the instrument that limit the voltage to 30 V
and the current to 0.5 A. The circuits protect the
user from unnecessary electrical hazards.
The TE70XP and the TE77XP have a built-in
power supply for transferring gels. This eliminates
the need for an external power supply. These
instruments can deliver up to 30 V and 0.5 A.
The instruments also monitor the transfer stack
resistance, and can stop a transfer if large changes
indicate that the buffer system is depleting.
Multiple gels can be transferred at the same time
by placing several small gels of the same thickness
side by side, or by stacking two gels vertically in a
carefully constructed multi-layered stack.
The electrodes are made of the best possible
materials to ensure the units last a long time.
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p1
Fig 1. Semi-dry transfer unit
main components.
power
cords
top electrode
power
transformer
lower electrode
TE77XP
voltage
leads
top electrode
lower electrode
TE70X
circuit breaker
reset